Advertisement

Evaluation of cutaneous wound healing activity of Ocimum basilicum aqueous extract ointment in rats

  • Mohammad Mahdi Zangeneh
  • Akram Zangeneh
  • Niloofar SeydiEmail author
  • Rohallah Moradi
Original Article

Abstract

The recent researches have revealed the potential of ethno-medicinal plants on the treatment of the cutaneous wound. The purpose of the study was to investigate the cutaneous wound healing property of aqueous extract of Ocimum basilicum. In our study, 120 Sprague-Dawley male rats were used. After creating the cutaneous wounds, the animals were randomly divided into four groups: untreated control, treatment with Eucerin ointment, treatment with 3% tetracycline ointment, and treatment with 3% O. basilicum aqueous extract ointment. On days 10, 20, and 30 after creating the wound, for biochemical and histopathological analysis of the cutaneous wound healing trend, a section was prepared from all dermal thicknesses. The data were analyzed by SPSS-22 software. O. basilicum aqueous extract ointment could significantly (p ≤ 0.05) reduce the levels of the wound area, total cell, and neutrophil, lymphocyte, and enhance the levels of wound contracture, hydroxyproline, hexosamine, hexuronic acid, and fibrocyte as compared to the basal ointment and control groups. In conclusion, the acquired findings showed the cutaneous wound healing property of O. basilicum aqueous extract ointment.

Keywords

Ocimum basilicum Aqueous extract Ointment Cutaneous wound 

Notes

Compliance with ethical standards

Conflict of interest

The authors declare that there is no conflict of interest.

Ethic approval

All institutional and national guidelines for the care and use of laboratory animals were followed.

References

  1. Azhdari-Zarmehri H, Nazemi S, Ghasemi E, Musavi Z, Tahmasebi Z, Farsad F, Farzam A (2014) Assessment of effect of hydro-alcoholic extract of scrophularia striata on burn healing in rat. JBUMS 16(5):42–48Google Scholar
  2. Caetano GF, Fronza M, Leite MN, Gomes A, Frade MAC (2016) Comparison of collagen content in skin wounds evaluated by biochemical assay and by computer-aided histomorphometric analysis. Pharm Biol 54(11):2555–2559CrossRefGoogle Scholar
  3. Dastmalchi K, Damien DH, Oinonena P, Darwisd Y, Laaksoa I, Hiltunen R (2008) Chemical composition and in vitro antioscidative activity of a lemon balm (Melissa Officinaliss L.) extract. LWT Food Sci Technol 41:391–400CrossRefGoogle Scholar
  4. Dong Y-L, Fleming RYD, Yan TZ, Herndon DN, Waymack JP (1993) Effect of ibuprofen on the inflammatory response to surgical wounds. J Trauma 35(3):340–343CrossRefGoogle Scholar
  5. Dwivedi D, Dwivedi M, Malviya S, Singh V (2017) Evaluation of wound healing, anti-microbial and antioxidant potential of Pongamia pinnata in wistar rats. J Tradit Complement Med 7(1):79–85CrossRefGoogle Scholar
  6. Foschi D, Trabucchi E, Musazzi M, Castoldi L, Di Mattia D, Radaelli E, Marazzi M, Franzini P, Berlusconi A (1988) The effects of oxygen free radicals on wound healing. Int J Tissue React 10(6):373–379Google Scholar
  7. Geethalakshmi R, Sakravarthi C, Kritika T, Arul Kirubakaran M, Sarada DVL (2013) Evaluation of antioxidant and wound healing potentials of Sphaeranthus amaranthoides Burm. f. Biomed Res Int 2013:607109CrossRefGoogle Scholar
  8. Ghashghaii A, Hashemnia M, Nikousefat Z, Zangeneh MM, Zangeneh A (2017) Wound healing potential of methanolic extract of Scrophularia striata in rats. Pharm Sci 23(4):256–263CrossRefGoogle Scholar
  9. Goorani S, Zangeneh MM, Koohi MK, Seydi N, Zangeneh A, Souri N, Hosseini MS (2018) Assessment of antioxidant and cutaneous wound healing effects of Falcaria vulgaris aqueous extract in Wistar male rats. Comp Clin Pathol 28:435–445.  https://doi.org/10.1007/s00580-018-2866-3 CrossRefGoogle Scholar
  10. Goorani S, Shariatifar N, Seydi N, Zangeneh A, Moradi R, Tari B, Nazari F, Zangeneh MM (2019) The aqueous extract of Allium saralicum R.M. Fritsch effectively treat induced anemia. Experimental study on Wistar rats. Orient Pharm Exp Med.  https://doi.org/10.1007/s13596-019-00361-5
  11. Guo S, DiPietro LA (2010) Factors affecting wound healing. J Dent Res 89(3):219–229CrossRefGoogle Scholar
  12. Hagh-Nazari L, Goodarzi N, Zangeneh MM, Zangeneh A, Tahvilian R, Moradi R (2017) Stereological study of kidney in streptozotocin-induced diabetic mice treated with ethanolic extract of Stevia rebaudiana (bitter fraction). Comp Clin Pathol 26(2):455–463CrossRefGoogle Scholar
  13. Hamelian M, Zangeneh MM, Amisama A, Varmira K, Veisi H (2018) Green synthesis of silver nanoparticles using Thymus kotschyanus extract and evaluation of their antioxidant, antibacterial and cytotoxic effects. Appl Organomet Chem 32(9):e4458CrossRefGoogle Scholar
  14. Hemmati S, Rashtiani A, Zangeneh MM, Mohammadi P, Zangeneh A, Veisi H (2019) Green synthesis and characterization of silver nanoparticles using Fritillaria flower extract and their antibacterial activity against some human pathogens. Polyhedron 158:8–14CrossRefGoogle Scholar
  15. Hosseinkhani A, Falahatzadeh M, Raoofi E, Zarshenas MM (2016) An evidence-based review on wound healing herbal remedies from reports of traditional Persian medicine. J Evid Based Complementary Altern Med 22(2):334–343CrossRefGoogle Scholar
  16. Jalalvand AR, Zhaleh M, Goorani S, Zangeneh MM, Seydi N, Zangeneh A, Moradi R (2019) Chemical characterization and antioxidant, cytotoxic, antibacterial, and antifungal properties of ethanolic extract of Allium Saralicum R.M. Fritsch leaves rich in linolenic acid, methyl ester. J Photochem Photobiol B 192:103–112.  https://doi.org/10.1016/j.jphotobiol.201 CrossRefGoogle Scholar
  17. Kaurinovic B, Popovic M, Vlaisavljevic S, Trivic S (2011) Antioxidant capacity of Ocimum basilicum L. and Origanum vulgare L. extracts. Molecules 16(9):7401–7414CrossRefGoogle Scholar
  18. Khair-ul-Bariyah S, Ahmed ID, Ikram M (2012) Ocimum basilicum: a review on phytochemical and pharmacological studies. Pak J Chem 2(2):78–85CrossRefGoogle Scholar
  19. Khorasgni A, Karimi AH, Nazem MR (2010) A comparison of healing effects of propolis and silver sulfadiazine on full thickness skin wounds in rats. Pak Vet J 30(2):72–740Google Scholar
  20. Koh TJ, DiPietro LA (2011) Inflammation and wound healing: the role of the macrophage. Expert Rev Mol Med 13:e23CrossRefGoogle Scholar
  21. Kumar B, Vijaykumar M, Govindarajan R, Pushpangadan P (2008) Ethnopharmacological approaches to wound healing. Exploring medicinal plants of India. J Ethnopharmacol 12(8):103–110Google Scholar
  22. Lazarus GS, Cooper DM, Knighton DR, Margolis DJ, Pecoraro RE, Rodeheaver G, Robson MC (1994) Definitions and guidelines for assessment of wounds and evaluation of healing. Arch Dermatol 130(4):489–493CrossRefGoogle Scholar
  23. Malekzadeh F (1986) Antimicrobial activity of Lawsonia inermis L. Appl Microbiol 16(4):663–664Google Scholar
  24. Moradi R, Hajialiani M, Salmani S, Almasi M, Zangeneh A, Zangeneh MM (2018) Effect of aqueous extract of Allium saralicum R.M. Fritsch on fatty liver induced by high-fat diet in Wistar rats. Comp Clin Pathol.  https://doi.org/10.1007/s00580-018-2834-y
  25. Mussel O, Saliva S, Costa A (2003) Mast cell in tissue response to density materials: an adhesive resin, a calcium hydroxide and a glass ionomer cement. J Cell Mol Med 7(2):171–173CrossRefGoogle Scholar
  26. Nayak BS, Isitor G, Davis EM, Pillai GK (2007) The evidence based wound healing activity of Lawsonia inermis Linn. Phytother Res 21(9):827–831CrossRefGoogle Scholar
  27. Oryan A, Tabatabaiei Naieni A, Moshiri A, Mohammadalipoor A, Tabandeh MR (2012) Modulation of cutaneous wound healing by silymarin in rats. J Wound Care 21(9):457–464CrossRefGoogle Scholar
  28. Phillips GD, Whitehe RA, Kinghton DR (1991) Initiation and pattern of angiogenesis in wound healing in the rats. Am J Anat 192(3):257–262CrossRefGoogle Scholar
  29. Rezvanipour M, Pourzadehhosseini F, Malekpour R, Zarabi A (2007) The effect of mummy on some indices of wound healing in mice. J of Kerman Univ of Med Sci 14(4):77–267Google Scholar
  30. Robards K, Prenzler PD, Tucker G, Swatsitang P, Glover W (1999) Phenolic compounds and their role in oxidative processes in fruits. Food Chem 66(4):401–436CrossRefGoogle Scholar
  31. Sayyedrostami T, Pournaghi P, Ebrahimi Vosta-Kalaeea S, Zangeneh MM (2018) Evaluation of the wound healing activity of Chenopodium botrys leaves essential oil in rats (a short-term study). J Essent Oil Bear Pl 21(1):164–174CrossRefGoogle Scholar
  32. Shakibaie D, Pasharavesh L, Khoshboo S, Kaboodi B (2007) The effect of the “Falcaria vulgaris” on deep skin wound remodeling time and skin tension power in rats. J Kermanshah Univ Med Sci 10(3):187–194Google Scholar
  33. Sherkatolabbasieh H, Hagh-Nazari L, Shafiezadeh S, Goodarzi N, Zangeneh MM, Zangeneh A (2017) Ameliorative effects of the ethanolic extract of Allium saralicum R.M. Fritsch on CCl4-induced nephrotoxicity in mice: a stereological examination. Arch Biol Sci 69(3):535–543CrossRefGoogle Scholar
  34. Souba WW, Wilmore D (1999) Diet and nutrition in case of the patient with surgery. 9th Ed. Williams and Wilkins Press, Baltimore, pp 1589–1618Google Scholar
  35. Warsi A, Sholichah R (2017) Phytochemical screening and antioxidant activity of ethanolic extract and ethyl acetate fraction from basil leaf (Ocimum basilicum L.) by DPPH radical scavenging method. Mater Sci Eng.  https://doi.org/10.1088/1757-899X/259/1/012008
  36. Zangeneh MM, Goodarzi N, Zangeneh A, Tahvilian R, Najafi F (2018a) Amelioration of renal structural changes in STZ-induced diabetic mice with ethanolic extract of Allium saralicum R.M. Fritsch. Comp Clin Pathol 27(4):861–867CrossRefGoogle Scholar
  37. Zangeneh MM, Zangeneh A, Tahvilian R, Moradi R (2018b) Antidiabetic, hematoprotective and nephroprotective effects of the aqueous extract of Falcaria vulgaris in diabetic male mice. Arch Bio Sci 70(4):655–664CrossRefGoogle Scholar
  38. Zangeneh MM, Zangeneh A, Tahvilian R, Moradi R, Zhaleh H, Amiri-Paryan A, Bahrami E (2018c) Hepatoprotective and hematoprotective effects of Falcaria vulgaris aqueous extract against CCl4-induced hepatic injury in mice. Comp Clin Pathol 27:1359–1365.  https://doi.org/10.1007/s00580-018-2747-9 CrossRefGoogle Scholar
  39. Zhaleh M, Sohrabi N, Zangeneh MM, Zangeneh A, Moradi R, Zhaleh H (2018) Chemical composition and antibacterial effects of essential oil of Rhus coriaria fruits in the west of Iran (Kermanshah). J Essent Oil Bear Plant 21(2):493–501CrossRefGoogle Scholar

Copyright information

© Springer-Verlag London Ltd., part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Clinical Science, Faculty of Veterinary MedicineRazi UniversityKermanshahIran
  2. 2.Biotechnology and Medicinal Plants Research CenterIlam University of Medical SciencesIlamIran

Personalised recommendations