Changes in the cardiac phosphotransfer network associated with oxidative stress in experimentally infected Brucella ovis mice
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The aim of this study was to evaluate whether Brucella ovis infection altered the activities of cardiac enzymes belonging to the phosphotransfer network and cause an oxidative imbalance in mice, used as an experimental model. We used hearts from 48 mice in two groups (n = 24 each): uninfected (control group) and infected by B. ovis, collected on days 7, 15, 30, and 60 post-infection (PI). Cardiac cytosolic creatine kinase (CK) activity was higher in infected animals than in the control group on days 30 and 60 PI, while cardiac mitochondrial CK activity was lower at the same moments of infection. Cardiac pyruvate kinase (PK) activity was lower in infected animals than in the control group on day 60 PI, while no significant difference was observed between groups regarding cardiac adenylate kinase (AK) activity. Cardiac thiobarbituric acid reactive substances (TBARS) were higher in infected animals than in the control group on days 30 and 60 PI, while non-protein thiol (NPSH) levels were lower on days 30 and 60 PI. Finally, cardiac SOD activity was higher in infected animals than in the control group on day 60 PI. The inhibition on mitochondrial CK activity caused an impairment of cardiac energy homeostasis, possibly through depletion of ATP production, although cytosolic cardiac CK activity acted in an attempt to restore the mitochondrial ATP levels through a feedback mechanism.
KeywordsEnergy metabolism Heart Oxidative stress Disease pathophysiology ATP
This study was supported by the fellowships from UNIEDU, Brazil. We also thank CNPq and CAPES for their financial support.
Compliance with ethical standards
This experiment was approved by the Animal Welfare Committee of the State University of Santa Catarina (UDESC) under protocol number 0015317/2017.
Conflict of interest
The authors declare that they have no conflict of interest.
- Baigún R, Conigliaro AS, Luna F (2000) Aislamiento de Brucella ovis y control de reaccionantes serológicos en epididimitis ovina. Vet Argent 17:103–107Google Scholar
- Misra HP, Fridovich I (1972) The role of superoxide anion in the autoxidation of epinephrine and a simple assay for superoxide dismutase. J Biol Chem 25:3170–3175Google Scholar
- Perin G, Jaguezeski AM, Miglioranza D, da Silva TMA, Stefani LM et al (2018) Hematological and biochemical disturbances caused by Brucella ovis infection using an experimental model. Comp Clin Pathol 518:1–8Google Scholar
- Serefhanoglu K, Taskin A, Turan H, Timurkaynak FE, Arslan H, Erel O (2009) Evaluation of oxidative status in patients with brucellosis. Braz J Infect Dis 113:249–251Google Scholar
- Vanderperre B, Bender T, Kunji ER, Martinou JC (2014) Import of mitochondrial pyruvate and its effects on homeostasis. Curr Opin Cell Biol 15:35–41Google Scholar