Comparative Clinical Pathology

, Volume 28, Issue 2, pp 531–540 | Cite as

Pathology, isolation and characterisation of virulent and diverse Mannheimia haemolytica and Pasteurella multocida associated with fatal pneumonia in sheep, Rajasthan, India

  • Fateh SinghEmail author
  • Ganesh Gangaram Sonawane
  • Rajendra Kumar Meena
Original Article


Pneumonia, a major sheep disease, causes high mortality among affected animals predominantly the lambs. It is caused by a number of microbes including Mannheimia haemolytica/Pasteurella multocida. It was aimed to explore the pathology in the lungs due to pneumonia, and to isolate and characterise the virulent M. haemolytica/P. multocida strains. The lung tissues and heart blood specimens were collected by necropsy of sheep (n = 96). These samples were used for isolation and characterisation of M. haemolytica/P. multocida. Moreover, the fresh and square-shaped lung pieces were also taken to perform the histopathological analysis. Grossly, the petechial and ecchymotic haemorrhages, congestion and consolidation in lung lobes were observed at necropsy. Pneumonia was diagnosed as acute fibrinous-bronchopneumonia (36), suppurative chronic bronchopneumonia (10), acute interstitial pneumonia (32) and acute pulmonary congestion (18) based on histological lesions. Bacterial isolation recovered M. haemolytica alone or along with P. multocida and/or Bibersteinia trehalosi identified by phenotypic characters and sequencing of 16S rRNA. Haemolytic colonies were documented to carry the PHSSA, plpE and Rpt2 genes. P. multocida identification at species level was carried out by KMT1 gene detection. These isolates were noted to carry the capsule types A (hyaD-hyaC gene) and D (dcbF gene) and outer membrane protein genes ompH and omp87. Sequencing revealed nucleotide variability in Rpt2, ompH and omp87 genes.We highlighted the occurrence of fatal ovine pneumonic pasteurellosis/mannheimiosis caused and/or complicated by virulent M. haemolytica/P. multocida strains.


Bacterial infections Mannheimia haemolytica Pasteurella multocida Pathology Pneumonia Virulence 



The authors are thankful to Dr. Dhirendra Singh, then Head of the Animal Health Division and the Director of ICAR-Central Sheep and Wool Research Institute, Avikanagar, Tonk, Rajasthan, India, for providing the necessary facilities to conduct the research work.

Compliance with ethical standards

Financial support

This research received no specific grant from any funding agency, commercial or not-for profit sectors.

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.


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Copyright information

© Springer-Verlag London Ltd., part of Springer Nature 2019

Authors and Affiliations

  1. 1.Animal Health DivisionICAR-Central Sheep and Wool Research InstituteTonkIndia
  2. 2.ICAR-National Institute of High Security Animal DiseasesBhopalIndia

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