Advertisement

Comparative Clinical Pathology

, Volume 28, Issue 2, pp 531–540 | Cite as

Pathology, isolation and characterisation of virulent and diverse Mannheimia haemolytica and Pasteurella multocida associated with fatal pneumonia in sheep, Rajasthan, India

  • Fateh SinghEmail author
  • Ganesh Gangaram Sonawane
  • Rajendra Kumar Meena
Original Article
  • 28 Downloads

Abstract

Pneumonia, a major sheep disease, causes high mortality among affected animals predominantly the lambs. It is caused by a number of microbes including Mannheimia haemolytica/Pasteurella multocida. It was aimed to explore the pathology in the lungs due to pneumonia, and to isolate and characterise the virulent M. haemolytica/P. multocida strains. The lung tissues and heart blood specimens were collected by necropsy of sheep (n = 96). These samples were used for isolation and characterisation of M. haemolytica/P. multocida. Moreover, the fresh and square-shaped lung pieces were also taken to perform the histopathological analysis. Grossly, the petechial and ecchymotic haemorrhages, congestion and consolidation in lung lobes were observed at necropsy. Pneumonia was diagnosed as acute fibrinous-bronchopneumonia (36), suppurative chronic bronchopneumonia (10), acute interstitial pneumonia (32) and acute pulmonary congestion (18) based on histological lesions. Bacterial isolation recovered M. haemolytica alone or along with P. multocida and/or Bibersteinia trehalosi identified by phenotypic characters and sequencing of 16S rRNA. Haemolytic colonies were documented to carry the PHSSA, plpE and Rpt2 genes. P. multocida identification at species level was carried out by KMT1 gene detection. These isolates were noted to carry the capsule types A (hyaD-hyaC gene) and D (dcbF gene) and outer membrane protein genes ompH and omp87. Sequencing revealed nucleotide variability in Rpt2, ompH and omp87 genes.We highlighted the occurrence of fatal ovine pneumonic pasteurellosis/mannheimiosis caused and/or complicated by virulent M. haemolytica/P. multocida strains.

Keywords

Bacterial infections Mannheimia haemolytica Pasteurella multocida Pathology Pneumonia Virulence 

Notes

Acknowledgements

The authors are thankful to Dr. Dhirendra Singh, then Head of the Animal Health Division and the Director of ICAR-Central Sheep and Wool Research Institute, Avikanagar, Tonk, Rajasthan, India, for providing the necessary facilities to conduct the research work.

Compliance with ethical standards

Financial support

This research received no specific grant from any funding agency, commercial or not-for profit sectors.

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

References

  1. Ayalew S, Blackwood ER, Confer AW (2006) Sequence diversity of the immunogenic outer membrane lipoprotein PlpE from Mannheimia haemolytica serotypes 1, 2, and 6. Vet Microbiol 31:260–268CrossRefGoogle Scholar
  2. Bell S (2008) Respiratory disease in sheep. In Pract 30:200–207CrossRefGoogle Scholar
  3. Black P, Nunn M (2009) Impact of climate change and environmental change on emerging and re-emerging animal diseases and animal production. Proceedings of technical items presented in 77th general session of the OIE world assembly of delegates, 24–29 May, Paris, France, pp 15Google Scholar
  4. Brogden KA, Lehmkuhl HD, Cutlip RC (1998) Pasteurella haemolytica complicated respiratory infections in sheep and goats. Vet Res 29:233–254Google Scholar
  5. Chakraborty S, Kumar A, Tiwari R, Rahal A, Malik Y, Dhama K, Pal A, Prasad M (2014) Advances in diagnosis of respiratory diseases of small ruminants. Vet Med Int 2014:508304CrossRefGoogle Scholar
  6. Chaudhuri P, Goswami PP (2001) Cloning of 87 kDa outer membrane protein gene of Pasteurella multocida P52. Res Vet Sci 70:255–256CrossRefGoogle Scholar
  7. CLSI (2008) Performance standards for antimicrobial disk and dilution susceptibility tests for bacteria isolated from animals- Document M31-A3, Wayne, Pennsylvania, USAGoogle Scholar
  8. CLSI (2010) Methods for antimicrobial dilution and disk susceptibility testing of infrequently isolated or fastidious bacteria-Document M45-A2, Wayne, Pennsylvania, USAGoogle Scholar
  9. Dabo SM, Confer AW, Hartson SD (2005) Adherence of Pasteurella multocida to fibronectin. Vet Microbiol 110:265–275CrossRefGoogle Scholar
  10. Dabo SM, Taylor JD, Confer AW (2007) Pasteurella multocida and bovine respiratory disease. Anim Health Res Rev 8:129–150CrossRefGoogle Scholar
  11. Dar LM, Darzi MM, Mir MS, Kamil SA, Rashid A, Abdullah S, Hussain SA, Rather FA, Parihar S (2014) Histopathological and histoenzymatic studies on bronchopneumonia in sheep. J Appl Anim Res 42:289–296CrossRefGoogle Scholar
  12. Dassanayake RP, Shanthalingam S, Herndon CN, Subramaniam R, Lawrence PK, Bavananthasivam J, Cassirer EF, Haldorson GJ, Foreyt WJ, Rurangirwa FR, Knowles DP, Besser TE, Srikumaran S (2010) Mycoplasma ovipneumoniae can predispose bighorn sheep to fatal Mannheimia haemolytica pneumonia. Vet Microbiol 145:354–359CrossRefGoogle Scholar
  13. Dassanayake RP, Shanthalingam S, Herndon CN, Subramaniam R, Herndon CN, Bavananthasivam J, Haldorson GJ, Foreyt WJ, Evermann JF, Herrmann-Hoesing LM, Knowles DP, Srikumaran S (2013) Role of Bibersteinia trehalosi, respiratory syncytial virus, and parainfluenza-3 virus in bighorn sheep pneumonia. Vet Microbiol 162:166–172CrossRefGoogle Scholar
  14. Davies RL, Maccorquodale R, Reilly S (2004) Characterisation of bovine strains of Pasteurella multocida and comparison with isolates of avian, ovine and porcine origin. Vet Microbiol 99:145–158CrossRefGoogle Scholar
  15. Deressa A, Asfaw Y, Lubke B, Kyule MW, Tefera G, Zessin KH (2010) Molecular detection of Pasteurella multocida and Mannheimia haemolytica in sheep respiratory infections in Ethiopia. Int J Appl Res Vet Med 8:101–108Google Scholar
  16. Dixit SK, Singh S, Tripathi BN, Singh F, Sonawane GG, Kumar J (2010) Therapeutic management of pneumonia in sheep. Vet Pract 11:111–113Google Scholar
  17. Gautam R, Kumar AA, Singh VP, Singh VP, Dutta TK, Shivachandra SB (2004) Specific identification of Pasteurella multocida serogroup A isolates by PCR assay. Res Vet Sci 76:179–185CrossRefGoogle Scholar
  18. Gonzalez C, Murtaugh MP, Maheswaran SK (1991) Genomic distribution of a serotype 1-specific antigen coding DNA fragment of Pasteurella haemolytica. J Vet Med B Infect Dis Vet Public Health 38:599–609CrossRefGoogle Scholar
  19. Gonzalez CT, Maheswaran SK, Murtaugh MP (1995) Pasteurella haemolytica serotype 2 contains the gene for a noncapsular serotype 1-specific antigen. Infect Immun 63:1340–1348Google Scholar
  20. Goodwin KA, Jackson R, Brown C, Davies PR, Morris RS, Perkins NR (2004) Pneumonic lesions in lambs in New Zealand: patterns of prevalence and effects on production. N Z Vet J 52:175–179CrossRefGoogle Scholar
  21. Harmon BG, Glisson JR, Latimer KS, Steffens WL, Nunnally JC (1991) Resistance of Pasteurella multocida A: 3, 4 to phagocytosis by turkey macrophages and heterophils. Am J Vet Res 52:1507–1511Google Scholar
  22. Harper M, Boyce JD, Adler B (2006) Pasteurella multocida pathogenesis: 125 years after Pasteur. FEMS Microbiol Lett 265:1–10CrossRefGoogle Scholar
  23. Hassan GM, El-Feky ZA, Eissa EA, Teleb AA (2016) Rapid diagnosis of virulent Pasteurella multocida isolated from farm animals with clinical manifestation of pneumonia respiratory infection using 16S rDNA and KMT1 gene. Asian Pac J Trop Dis 6:21–26CrossRefGoogle Scholar
  24. Hawari AD, Hassawi DS, Sweiss M (2008) Isolation and identification of Mannheimia haemolytica and Pasteurella multocida in sheep and goats using biochemical tests and random amplified polymorphic DNA (RAPD) analysis. J Biol Sci (7):1251–1254Google Scholar
  25. Kumar J, Dixit SK, Kumar R (2015) Rapid detection of Mannheimia haemolytica in lung tissues of sheep and from bacterial culture. Vet World 8:1073–1077CrossRefGoogle Scholar
  26. Lane DJ (1991) 16S/23S rRNA sequencing. In: Stackenbrandt E, Goodfellow M (eds) Nucleic acid techniques in bacterial systematics. Wiley, Chichester, pp 115–175Google Scholar
  27. Lin J, Huang S, Zhang Q (2002) Outer membrane proteins: key players for bacterial adaptation in host niches. Microbes Infect 4:325–331CrossRefGoogle Scholar
  28. Luna LG (1968) Manual of histologic staining methods of the Armed Forces. Institute of Pathology, New YorkGoogle Scholar
  29. Marru HD, Anijajo TT, Hassen AA (2013) A study on ovine pneumonic pasteurellosis: isolation and identification of pasteurellae and their antibiogram susceptibility pattern in Haramaya district, Eastern Hararghe, Ethiopia. BMC Vet Res 9:239CrossRefGoogle Scholar
  30. Miller MW (2001) Pasteurellosis. In: Williams ES, Barker IK (eds) Infectious diseases of wild mammals. Iowa State University Press, Ames, p 558Google Scholar
  31. Mohamed RA, Abdelsalam EB (2008) A review on pneumonic Pasteurellosis (respiratory Mannheimiosis) with emphasis on pathogenesis, virulence mechanism and predisposing factors. Bulg J Vet Med 11:139–160Google Scholar
  32. Odugbo MO, Odamab LE, Umohc JU, Lamorde AG (2006) Pasteurella multocida pneumonic infection in sheep: prevalence, clinical and pathological studies. Small Rumin Res 66:273–277CrossRefGoogle Scholar
  33. Ryan KA, Lo RY (1999) Characterization of a CACAG pentanucleotide repeat in Pasteurella haemolytica and its possible role in modulation of a novel type III restriction-modification system. Nucleic Acids Res 27:1505–1511CrossRefGoogle Scholar
  34. Sarangi LN, Thomas P, Gupta SK, Priyadarshini A, Kumar S, Nagaleekar VK, Kumar A, Singh VP (2015) Virulence gene profiling and antibiotic resistance pattern of Indian isolates of Pasteurella multocida of small ruminant origin. Comp Immunol Microbiol Infect Dis 38:33–39CrossRefGoogle Scholar
  35. Singh R, Tewari K, Packiriswamy N, Marla S, Rao VDP (2011) Molecular characterization and computational analysis of the major outer membrane protein (OmpH) gene of Pasteurella multocida P52. Vet Arh 81:211–222Google Scholar
  36. Singh F, Sonawane GG, Meena RK (2018) Molecular detection of virulent Mannheimia haemolytica and Pasteurella multocida in lung tissues of pneumonic sheep from semiarid tropics, Rajasthan, India. Turk J Vet Anim Sci 42:556–561CrossRefGoogle Scholar
  37. Townsend KM, Frost AJ, Lee CW, Papadimitriou JM, Dawkins HJS (1998) Development of PCR assays for species and type specific identification of Pasteurella multocida isolates. J Clin Microbiol 36:1096–1100Google Scholar
  38. Townsend KM, Boyce JD, Chung JY, Frost AJ, Adler B (2001) Genetic organization of Pasteurella multocida cap loci and development of a multiplex capsular PCR typing system. J Clin Microbiol 39:924–929CrossRefGoogle Scholar
  39. Valadan M, Jabbari AR, Niroumand M, Tahamtan Y, Bani Hashemi SR (2014) Isolation and identification of Pasteurella multocida from sheep & goat in Iran. Arch Razi Inst 69:47–55Google Scholar
  40. Watson PJ, Davies RL (2002) Outbreak of Pasteurella multocida septicaemia in neonatal lambs. Vet Rec 151:420–422CrossRefGoogle Scholar

Copyright information

© Springer-Verlag London Ltd., part of Springer Nature 2019

Authors and Affiliations

  1. 1.Animal Health DivisionICAR-Central Sheep and Wool Research InstituteTonkIndia
  2. 2.ICAR-National Institute of High Security Animal DiseasesBhopalIndia

Personalised recommendations