Histomorphogenesis on hindbrain in Huso huso (Beluga sturgeon) larvae

  • Sherma TavighiEmail author
  • Zohreh Saadatfar
  • Bahador Shojaei
  • Morteza Behnam Rassouli
Original Article


This study has been done on evolution and morphology of hindbrain structure, during larvae life from 1 to 54 dph on Huso huso. It was concentrated on significant cerebellum and medulla oblongata. The cerebellum occupied the rostral part of dorsal wall of fourth ventricle. The fourth ventricle was visible on 1 day old and by aging was majorly. The fourth ventricle lied on the caudally and continued into central canal (cc) in spinal cord. Cortex of cerebellum had three layers, which were visible from 6 days old. Granular layer of corpus cerebelli, eminentia granularis, and less valvula cerebelli contained Golgi type II, and purkinje cells and observed from 6 days old clearly. Three sulci recognized in cerebellum on 15 days old. Medulla oblongata located at the caudal part of cerebellum and observed from 1 day old and contained eminentia granulares and crista cerebellares. Nucleuses of facial and vagal nerves were in ventral thick part of medulla oblongata. This structure, similar to cerebellum had columns of columnar cells in its gray matter. The white and gray matter, in hindbrain was visible on 1 and 3 days old, and from 6 days old the rate of white matter increased. Stereological results showed that there were significantly distinctive regional differences in the volume of different parts of hindbrain represented a correlation and was P < 0.05. Generally, in H. huso larvae from 1 to 54 days old, the volume of hindbrain had a significant increased such that it had the largest volume in fish brain compare to other parts.


Hindbrain H. Huso Evolution Volume 



Thanks to Dr. Tavighi for practical assistance and labor on the thesis, Dr. Shojaei for embryology information, and Dr. Behnam Rassouli for stereological technique.

Author contributions

All authors contributed equally to this work and are responsible for the data presented herein.


The study was supported by the Faculty of Veterinary Science Ferdowsi University of Mashhad, Iran, (Grant number455).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of Interest.

Ethical approval

The study protocol was approved by the Animal Care and Use Committee, Faculty of Veterinary Science, Ferdowsi University, Iran (Animal Use Protocol No.140).

Human and animal rights

This article does not contain any studies with human participants performed by any of the authors.


  1. Bauchot R, Bauchot ML, Platel R, Ridet JM (1977) Brains of Hawaiian tropical fishes; brain size and evolution. Copeia 1977:42–46CrossRefGoogle Scholar
  2. Brandstatter R, Kotrschal K (1989) Life history of roach, Rutilus rutilus (cyprinidae, Teleostei). Brain Behav Evol 34:35–42CrossRefGoogle Scholar
  3. Broglio C, Rodriquez R, Salas C (2003) Spatial cognition and its neural basis in teleost fishes. Fish Fisher 4:274–255CrossRefGoogle Scholar
  4. Burr HS (1928) The central nervous system of Orthagoriscus mola. J Comp Neurol 45:33–128CrossRefGoogle Scholar
  5. Butler AB, Hodos W (2005) Comparative vertebrate neuroanatomy, evolution and adaptation, 2th edition, United States of AmericaGoogle Scholar
  6. Davis RE, Northcutt RG (eds) (1983) Fish neurobiology, vol II. The university of Michigan Press, Ann ArborGoogle Scholar
  7. Eaton RC, Lee RK, Foreman MB (2001) The Mauthner cell and other identified neurons of the brainstem escape network of fish. Prog Neurobiol 63:467–485CrossRefGoogle Scholar
  8. Evans HM (1935) The brain of Gadus, with special reference to the medulla oblongata and its variations according to the feeding habits if different Gadidae – I. Proc R Soc Lond B Biol Sci 117B:367–399Google Scholar
  9. Evans H M (1940) Brain and body fish. A Study of Brain Pattern in Relation to Hunting and Feeding in Fish London: The Technical Press Ltd pp 164Google Scholar
  10. Finger TE (1988) Organization of chemosensory systems within the brains of bony fish. In: Atema J, Fay RR, Popper AN, Tavolga WN (eds) Sensory biology of aquatic animals. Speringer-Verlag, New York, NY, pp 339–364CrossRefGoogle Scholar
  11. Gibbs MA, Northcutt RG (2004) Development of the lateral line system in the shovelnose sturgeon. Brain Behav Evol 64:70–84CrossRefGoogle Scholar
  12. Gundersen HJG, Jensen EB (1987) The efficiency of systematic sampling in stereology and its prediction. J Microsc 147:263–229CrossRefGoogle Scholar
  13. Helfman G, Collette B, Facey D (1997) The diversity of fishes, vol 544. Blackwell publishing, LondonGoogle Scholar
  14. Hernando J A, Domezain A, Zabala C, Domezain J, Cabrera R, Soriguer M C (2005) Desarrollo de las aletas pectorales durante la morfogenesis de Acipenser naccarii, Bonaparte 1836. In: IX Congreso Nacional de Acuiculture. Abstract Book. Junta de Andalucia, Cadiz, pp 81–85Google Scholar
  15. Huesa G, Anadon R, Yanez J (2003) Afferent and efferent connections of the cerebellum of the chondrostean Acipenser baerii. A carbocyanine dye (DiL) tracing study. J Comp Neurol 460:327–344CrossRefGoogle Scholar
  16. Ito H, Ishikawa Y, Yoshimoto M, Yoshimoto N (2007) Diversity of brain morphology in teleost: brain and ecological niche. Brain Behav Evol 69:76–86CrossRefGoogle Scholar
  17. Johnston JB (1898) Hindbrain and cranial nerves of Acipenser. Anat Anz 14:580–602Google Scholar
  18. Kimley AP, Beavers SC, Curtis TH, Jorgensen SJ (2002) Movements and swimming behavior of three species of sharks in La Jolla Canyon, California. Environ Biol Fishes 63:117–135CrossRefGoogle Scholar
  19. Kotrschal K, Van Staaden MJ, Huber R (1998) Fish brains: evolution and environmental relationships. Rev Fish Biol Fish 8:373–408CrossRefGoogle Scholar
  20. Lauder GV, Liem K (1983) The evolution and inter relationships of the actinopterygian fishes. Bull Mus Comp Zool 150:95–197Google Scholar
  21. McCormik CA (1982) The organization of the octavolateralis area in actinopterygian fishes: a new interpretation. J Morphol 171:159–181CrossRefGoogle Scholar
  22. Meek J, Nieuwenhuys R (1998) Holosteans and teleosts. In: Nieuwenhuys R, Ten Donkelaar HJ, Nicholson C (eds) The central nervous system of vertebrates. Springer, Berlin, pp 760–937Google Scholar
  23. Nieuwnhuys R (1967) Comparative anatomy of cerebellum. Prog Brain Res 15:1–93Google Scholar
  24. Nieuwnhuys R (1998) Chondrostean fishes. In: Nieuwnhuys R, Ten Donkelaar HJ, Nicholson C (eds) The central nervous system of vertebrates. Speringer Verlag, Berlin, pp 701–757CrossRefGoogle Scholar
  25. Norris HW (1925) Observations upon the peripheral distribution of the cranial nerves of certain ganoids fishes (Amia, Lepidosteus, Polyodon, Scaphyrrinchus, and Acipenser). J Comp Neurol 39:345–432CrossRefGoogle Scholar
  26. Northcutt RG (1978) Brain organization in the cartilaginous fishes. In: Hodgson ES, Mathewson RF (eds) Sensory biology of sharks, skates and rays. Office of Naval Research, Arlington, pp 117–193Google Scholar
  27. Northcutt RG (1996) The agnathan ark: the origin of craniate brains. Brain Behav Evol 48:237–247CrossRefGoogle Scholar
  28. Northcutt RG, Davis RE (1983) Fish neurobiology. University of Michigan Press, Ann ArborGoogle Scholar
  29. Popper AN, Fay RR (1993) Sound detection and processing by fish: critical review and major research questions. Brain Behav Evol 41:14–38CrossRefGoogle Scholar
  30. Pouwels E (1978) On the development of the cerebellum of the trout, Salmo gairdneri. I. Patterns of cell migration. Anat Embryol 152:291–308CrossRefGoogle Scholar
  31. Singh CP (1972) A comparative observation of the brain of some Indian freshwater teleost, with special reference to their feeding habits. Anat Anz 131:377–422Google Scholar
  32. Teeter JH, Szamier RB, Bennet MVL (1980) Ampullary electroreceptors in the sturgeon Scaphirhynchus platorynchus (Rafinesque). J Comp Physiol 138:213–233CrossRefGoogle Scholar
  33. Theunissen F (1914) The arrangements of the motor roots and nuclei in the brain of Acipenser ruthenus and Lepisosteus osseus. Proc Kon Ned Akad Wet (Amsterdam) 16:1032–1041Google Scholar
  34. Vazquez M, Rodriguez F, Domezain A, Salas C (2002) Development of the brain of the sturgeon Acipenser naccarii. Piscifactoria de Sierra Nevada, Riofrio, Granada, Spain. Appl Ichthyol 18:275–279CrossRefGoogle Scholar
  35. Wagner HJ (2003) Volumetric analysis of brain areas indicates a shift in sensory orientation during development in the deep-sea grenadier Coryphaenoides armatus. Mar Biol 142:791–797CrossRefGoogle Scholar

Copyright information

© Springer-Verlag London Ltd., part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Anatomical Science, Faculty of Veterinary MedicineFerdowsi university of MashhadMashhadIran
  2. 2.Department of Basic Sciences, Faculty of Veterinary MedicineShahid Bahonar University of KermanKermanIran
  3. 3.Department of Physiological Science, Faculty of BiologyFerdowsi university of MashhadMashhadIran

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