Trichostatin A (TSA) levels in response to treatment of rainbow trout with gamma-irradiated trophonts against Ichthyophthirius multifiliis
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Ichthyophthirius multifiliis is a large ciliated protozoan parasite that is widely distributed in freshwater fish. Histone deacetylase inhibitors (HDACi), e.g., trichostatin A (TSA), are recognized as new agents for the treatment of a wide range of parasitic diseases. In this study, TSA levels induced by gamma-irradiated trophonts and alginate/calcium phosphate nanoparticles were measured after 30 days following treatment against I. multifiliis in spleen, kidney, and liver tissues of rainbow trout. This sounds like TSA is an anti-parasitic enzyme that can increase in spleen, kidney, and liver tissues following treatment of rainbow trout against I. multifiliis (P < 0.05). Moreover, the results obtained from this study showed that the TSA activity level is tissue-dependent. The highest significance levels for TSA activity were found in spleen tissue of treated and healthy groups compared with the levels of the both liver and kidney tissues (P < 0.05).
KeywordsTrichostatin A (TSA) Rainbow trout Ichthyophthirius multifiliis Histone deacetylase inhibitors (HDACi) Nanoparticles
This paper presents results from the FAO/IAEA Coordinated Research Project (IAEA-CRP no. 16179/R0). The authors are grateful for the financial support provided by the International Atomic Energy Agency (IAEA), Vienna, Austria, and the Nuclear Science and Technology Research Institute, Karaj, Iran, and also the Department of Fisheries, University of Agricultural Sciences and Natural Resources, Sari, Mazandaran, Iran.
Compliance with ethical standards
All procedures were submitted to and approved by the Ethics Committee on Animal Experimentation of the University of Tehran.
Conflict of interest
The authors declare that they have no conflicts of interest.
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