Comparative Clinical Pathology

, Volume 28, Issue 1, pp 83–87 | Cite as

Epidemiology of cattle amphistomiasis in Gilan province, north of Iran

  • Ali Nikpay
  • Elham HoushmandEmail author
  • Ali Eslami
  • Saied Bokaie
Original Article


Amphistomiasis is a widespread disease of ruminants in many parts of the world including Iran. The aim of this study was to determine the prevalence and intensity of amphistomiasis in cattle from Gilan province, north of Iran in association with sex, age, breed, and season. This investigation was carried out over a 12-month period in Gilan. Through seasonal sampling and visual examination for amphistomiasis, the rumens and reticulum of 406 cattle, slaughtered in Rasht abattoir, Gilan province, were collected and relevant data on sex, age, and breed were recorded. The total number of harvested amphistomes, which was present in each organ was collected, counted, and preserved in a 10% formaldehyde solution. After preparation and identification, our findings revealed that Carmyerius spatiosus, Paramphistomum gotoi, Gastrothylax crumenifer, and Calicophoron calicophorum were present in this study. The overall prevalence of infection was 19.70% with a mean intensity of 764.70 (ranged from 10 to 6200). The relation between prevalence and season (P < 0.05) was significant but not sex, breed, and age (P > 0.05). On the other hand, the relative mean intensity had a positive relation with the breed (P = 0.050) and age (P < 0.01), but not with season (P = 0.108) and sex (P = 0.344). The results of this study can be used to plan for the control and prevention of this disease.


Epidemiology Amphistomiasis Cattle Gilan Iran 


Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.


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© Springer-Verlag London Ltd., part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Pathobiology, Faculty of Veterinary MedicineAmol University of Special Modern TechnologyMazandaranIran
  2. 2.Department of Parasitology, Faculty of Veterinary Medicine, Rasht BranchIslamic Azad UniversityGilanIran
  3. 3.Department of Parasitology, Faculty of Veterinary MedicineTehran UniversityTehranIran
  4. 4.Department of Food Hygiene and Control, Faculty of Veterinary MedicineTehran UniversityTehranIran

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