Haematological parameters of a monitor lizard
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The aims of this study were to collect blood samples using the lateral caudal vein and to determine the haematological parameters of monitor lizard. Apparently, healthy monitor lizard was used for the purpose of this study. Blood sample was collected into sterile sample bottle containing ethylenediaminetetraacetic acid (EDTA) as anticoagulant over a period of 15 days at 3-day intervals. The lizard was screened for haemoparasites. Microhaematocrit and thin blood smear techniques were used for the haematological analyses. Haemoglobin concentration (Hb) was determined using the cyanmethaemoglobin method. Mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH) and mean corpuscular haemoglobin concentration (MCHC) were calculated. There was no statistically significant difference (P < 0.05) in the packed cell volume (PCV), total red blood cells (RBC) and total white blood cells (WBC) counts which recorded values of 25.17 ± 2.44%, 3.9 ± 0.09 × 1012/l, and 9.57 ± 1.68 × 109/l respectively. No statistically significant difference (P < 0.05) was recorded for MCV, MCH, and MCHC with mean values of 64.48 ± 5.68 fl, 21.48 ± 1.90 pg and 33.30 ± 0.03 g/dl respectively. To the best of our knowledge, this is the first report on the haematological parameters of monitor lizard in Nigeria.
KeywordsBlood sample Monitor lizard Thin blood smear Packed cells volume Red blood cells
Compliance with ethical standards
Conflict of interest
The authors declare that there is no conflict of interest.
This article contains studies with an animal performed by any of the authors and ethical approval for this study was provided by the Ahmadu Bello University Committee on Animal Use and Care (ABUCAUC).
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