Comparative Clinical Pathology

, Volume 28, Issue 2, pp 369–376 | Cite as

Short-term effect of low-, moderate-, and high-intensity exercise training on cerebral dopamine neurotrophic factor (CDNF) and oxidative stress biomarkers in brain male Wistar rats

  • Hossein ShirvaniEmail author
  • Jalil Aslani
  • Ziya Fallah Mohammadi
  • Ehsan Arabzadeh
Original Article


Neurotrophic factors and exercise training are effective in the growth and survival of neuronal cells. These factors play a protective role against oxidative stress damage and have the same function as antioxidants. The purpose of this study was to investigate the effect of a session of endurance training with three different intensities on CDNF, SOD, and MDA levels of cerebral cortex in male rats. Thirty-two male Wistar rats (aged 20 weeks) were divided randomly into two control and training groups. The training group consisted of low-, moderate-, and high-intensity trainings. The training groups, after getting familiarization with the rodent treadmill, were dealt with an acute training session with three different intensities. The CDNF level of cerebral cortex was measured by ELISA assay, and the SOD and MDA levels of cerebral cortex by spectrophotometery. A significant difference was seen in the CDNF level between low- and high-intensity groups, as well as between high-intensity groups and control group (P = 0.001). The levels of SOD were increased significantly among all groups (except for control and low-intensity groups). The acute training with different intensities significantly prevents the increase in MDA level of cerebral cortex (P = 0.005). The result of this study shows that the physical exercise even in the short term can affect the protective factors and antioxidant system in neuronal cells. However, the benefits of high-intensity training were higher than others. Therefore, suggested that the role of acute exercise with different intensities should be carefully considered for the preconditioning against neuronal degenerative diseases.


Endurance training CDNF SOD MAD 



Cerebral dopamine neurotrophic factor


Superoxide dismutase





We acknowledge the Department of Exercise Physiology, University of Mazandaran for their contributions.

Compliance with ethical standards

This study was conducted according to the Declaration of Helsinki guidelines and approved by the Ethical Committee of Mazandaran University (Ethical cod #IR.BMSU.REC.1395.987).

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer-Verlag London Ltd., part of Springer Nature 2019

Authors and Affiliations

  1. 1.Exercise Physiology Research Center, Life Style InstituteBaqiyatallah University of Medical SciencesTehranIran
  2. 2.Faculty of Sport SciencesUniversity of MazandaranBabolsarIran

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