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Comparative Clinical Pathology

, Volume 28, Issue 2, pp 359–368 | Cite as

Pathology and immunohistochemical evaluation of Vibrio alginolyticus infection in Macrobrachium rosenbergii

  • Abdullateef AjadiEmail author
  • M. Y. Sabri
  • J. A. Atata
  • O. B. Daodu
  • B. O. Emikpe
Original Article
  • 23 Downloads

Abstract

Vibriosis is one of the common bacterial diseases plaguing the prawn and shrimp industry. However, the sequential histopathological changes associated with Vibrio alginolyticus-induced vibriosis is scanty in literature. This study investigated the histological alterations in groups of Macrobrachium rosenbergii experimentally injected intramuscularly between the second and third abdominal segment with 50 μL of 107 CFU of V. alginolyticus. Twenty-four hours post inoculation, the organism was isolated and molecularly characterized while infected giant freshwater prawns were observed for histological alterations and immunoreactivity in different tissues due to the experimental challenge.

The lesions observed in the organs include incipient haemocytic infiltration into the interstitial space of the tubules of the hepatopancreas and loss of epithelial layer, muscular necrosis and disruption of muscular layer with haemocytic infiltration, hyperplasia of epithelial cells and degeneration of the epithelium, deformed and necrotizing lamellae, and enlargement of the lamellar sinus of the gill, and the heart is characterized by localized nodular haemocytic reaction and melanization. Organs such as the heart, gill, hepatopancreas and muscle following injection of the putative bacteria showed immunohistochemical activity. Hence, histology and immunohistochemistry still remain important tools for vibriosis disease diagnoses in giant freshwater prawns.

Keywords

Pathology Immunohistochemistry Macrobrachium rosenbergii Vibrio alginolyticus 

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Copyright information

© Springer-Verlag London Ltd., part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Veterinary Pathology, Faculty of Veterinary MedicineUniversity of IlorinIlorinNigeria
  2. 2.Department of Veterinary Pathology and Microbiology, Faculty of Veterinary MedicineUniversiti Putra MalaysiaSerdangMalaysia
  3. 3.Department of Veterinary Microbiology, Faculty of Veterinary MedicineUniversity of IlorinIlorinNigeria
  4. 4.Department of Veterinary Pathology, Faculty of Veterinary MedicineUniversity of IbadanIbadanNigeria

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