Phylogenetic evaluation of Macrorhabdus ornithogaster isolated from a case of canary (Serinus canaria)
- 18 Downloads
Two dead birds from a flock of domestic canaries (Serinus canaria) were referred with a history of anorexia, weight loss, abdominal distension, and occasional mortality. A slightly enlarged liver and proventriculus were seen on the examination of the abdominal cavity. A large number of yeast resembling Macrorhabdus ornithogaster were observed on gram staining of the proventriculus mucosa. Pseudomonas aeruginosa was isolated on the microbial culture of the liver. The complete genomic sequence of the internal transcribed spacer 1 (ITS1) was determined in Macrorhabdus ornithogaster using panfungal primers. The alignment of the sequence obtained from this study with 6 GenBank deposited ITS1sequences of Macrorhabdus ornithogaster showed some repeated nucleotide substitutions. The complete ITS1 sequence of our study (MF993119) displayed a whole genetic similarity to ITS1 sequences of Macrorhabdus ornithogaster isolated from canaries and finches and clustered into a different group from ITS1 sequences of Macrorhabdus ornithogaster isolated from pssitacines. The ITS1 region is proposed as taxonomically informative for Macrorhabdus ornithogaster isolates from different hosts.
KeywordsMacrorhabdus ornithogaster Pseudomonas aeruginosa Serinus canaria Phylogeny
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflicts of interest.
All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.
- Madani SA, Haddad-Marandi MR, Arabkhazaeli F (2015) Concurrent atypical diffused tuberculosis and macrorhabdosis in a canary (Serinus canaria). Vet Res Forum 6(1):89–93Google Scholar
- Martins NR, Horta AC, Siqueira AM, Lopes SQ, Resende JS, Jorge MA, Assis RA, Martins NE, Fernandes AA, Barrios PR, Costa TJ (2006) Macrorhabdus ornithogaster in ostrich, rhea, canary, zebra finch, free range chicken, turkey, guinea-fowl, columbina pigeon, toucan, chuckar partridge and experimental infection in chicken, japanese quail and mice. Arq Bras Med Vet 58(3):291–298CrossRefGoogle Scholar
- Phalen DN (2016) Macrorhabdus Ornithogaster. In: Samour J (ed) Avian Medicine. Elsevier, Amsterdam, pp 473–477Google Scholar
- Piasecki T, Prochowska S, Celmer Ż, Sochacka A, Bednarski M (2012) Occurrence of Macrorhabdus ornithogaster in exotic and wild birds in Poland. Med Weter 68(4):245–249Google Scholar
- Quinn PJ, Carter ME, Markey B, Carter GR (1994) Veterinary clinical microbiology. Wolfe Publication, London, UK, pp 237–242Google Scholar
- White TJ, Bruns T, Lee SJ, Taylor JL (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR Protocols 18(1):315–322Google Scholar