Comparative Clinical Pathology

, Volume 28, Issue 1, pp 271–274 | Cite as

Morphological and molecular identification of Acanthocheilonema reconditum in a canine

  • A. M. EngelmannEmail author
  • A. S. Schafer
  • C. L. Lhamas
  • G. L. Dornelles
  • J. F. Cargnelutti
  • R. A. N. Ramos
  • S. G. Monteiro
  • C. M. Andrade
Case Report


Acanthocheilonema reconditum (A. reconditum) is a nematode with low pathogenicity that causes canine filariasis, without clinical signs. This filarid produces microfilariae that circulate in the blood of their natural hosts. Microfilariae of A. reconditum can be easily confused with microfilariae of Dirofilaria immitis (D. immitis) which causes a severe disease in dogs. The aim of this article is to describe a case of A. reconditum infection in a dog, diagnosed by fine needle aspiration cytology (FNAC) of two cutaneous nodules, blood smear examination, and further confirmed by molecular assay (PCR). This report alerts veterinarians to the presence of A. reconditum in the region of Santa Maria, Rio Grande do Sul, Brazil, and highlights the importance of performing the differentiation of microfilaria species. In addition, it demonstrates the importance of fine needle aspiration cytology as a diagnostic tool.


Dipetalonema reconditum Filarial nematodes Microfilariae Dog 


Funding information

This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior – Brasil (CAPES) – Finance Code 001.

Compliance with ethical standards

Conflict of interest

The authors declare that there are no conflicts of interest.

Ethical approval

Not applicable. To report this case, we had the consent of the guardian of the animal and the veterinarians who participated in the case.


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Copyright information

© Springer-Verlag London Ltd., part of Springer Nature 2018

Authors and Affiliations

  • A. M. Engelmann
    • 1
    Email author
  • A. S. Schafer
    • 1
  • C. L. Lhamas
    • 1
  • G. L. Dornelles
    • 1
  • J. F. Cargnelutti
    • 2
  • R. A. N. Ramos
    • 3
  • S. G. Monteiro
    • 4
  • C. M. Andrade
    • 1
  1. 1.Laboratório de Patologia Clínica Veterinária, Departamento de Pequenos Animais, Hospital Veterinário UniversitárioUniversidade Federal de Santa Maria (UFSM)Santa MariaBrazil
  2. 2.Laboratório de Virologia, Departamento de Medicina Veterinária Preventiva (DMVP), Centro de Ciências Rurais (CCR)Universidade Federal de Santa Maria (UFSM)Santa MariaBrazil
  3. 3.Unidade Acadêmica de GaranhunsUniversidade Federal Rural de PernambucoGaranhunsBrazil
  4. 4.Laboratório de Parasitologia Veterinária, Departamento de Microbiologia e ParasitologiaUniversidade Federal de Santa Maria (UFSM)Santa MariaBrazil

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