Comparative Clinical Pathology

, Volume 28, Issue 4, pp 985–995 | Cite as

Saccharomyces cerevisiae inhibits growth and metastasis and stimulates apoptosis in HT-29 colorectal cancer cell line

  • Roshanak Sambrani
  • Jalal AbdolalizadehEmail author
  • Leila Kohan
  • Behboud Jafari
Original Article


Colorectal cancer (CRC) is one of the leading causes of cancer-related death worldwide. Accumulating evidence has suggested that probiotics affect cellular pathways and specific genes involved in slow growth. Probiotics reduce the impact or stop the growth of cancer cells and tumors in animal models and human cell lines. The aim of this study was to investigate the effect of Saccharomyces cerevisiae (S. cerevisiae) on cell growth, metastasis, and apoptosis of the HT-29 colon cancer cell line. The HT-29 cells and S. cerevisiae were co-cultured in order to study the effects of S. cerevisiae on cell apoptosis, growth, and metastasis using 4,6-diamidino-2-phenylindole (DAPI) staining, 3-(4,5-dimethylthiazoyl-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, and scratch wound-healing assay, respectively. Real-time PCR was applied to evaluate the expression levels of Akt/PTEN, MAPK, and NF-ĸB genes. The supernatant obtained from S. cerevisiae in HT-29 cell line increased the expression of PTEN and Caspas3 genes in the first 24 h while the Bclxl and RelA genes showed decreased expression. By using MTT method after 48 h of treatment HT-29 cells with supernatant of S. cerevisiae, about 75% of the cells showed stopped growing. Therefore, it could be concluded that S. cerevisiae inhibits the growth of the HT-29 cells by inducing apoptosis and reducing metastasis.


Apoptosis HT-29 Probiotics Saccharomyces cerevisiae 



This work was supported by Tabriz University of Medical Sciences and Islamic Azad University, Marvdasht.

Compliance with ethical standards

Conflicts of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Informed consent

This article does not need any informed consent.


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Copyright information

© Springer-Verlag London Ltd., part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Genetic, Fars Science and Research BranchIslamic Azad UniversityMarvdashtIran
  2. 2.Department of Genetic Branch MarvdashtIslamic Azad UniversityMarvdashtIran
  3. 3.Drug Applied Research CenterTabriz University of Medical SciencesTabrizIran
  4. 4.Department of Biology, Arsanjan BranchIslamic Azad UniversityArsanjanIran
  5. 5.Department of Microbiology Ahar BranchIslamic Azad UniversityAharIran

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