Comparative Clinical Pathology

, Volume 28, Issue 4, pp 963–969 | Cite as

Hepatoprotective potential of aqueous extract of Allium eriophyllum Boiss in high-fat diet-induced fatty liver diseases

  • Samaneh Goorani
  • Mohsen Zhaleh
  • Majid Hajialiani
  • Rohallah Moradi
  • Mohammad Kazem Koohi
  • Khodabakhsh Rashidi
  • Mohammad Mahdi ZangenehEmail author
  • Akram Zangeneh
Original Article


In recent years, Iranian traditional medicine has been used to control, prevent, and treat various diseases such as fatty liver. One of these plants is Allium eriophyllum Boiss. In this research, we assessed the potential of aqueous extract of A. eriophyllum in the treatment of fatty liver disease in Wistar male rats. At beginning of the study, a total of 10 rats were selected as the negative control, and 50 rats were treated with a high-fat diet for 4 months. Then, the animals were randomly divided into six subgroups, including negative healthy control, untreated negative control, and four groups receiving the aqueous extract of A. eriophyllum at 25, 50, 100, and 200 mg/kg concentrations. After 2 months, the rats were sacrificed, and blood and liver samples were collected. The data were analyzed by SPSS 21 software. All groups of A. eriophyllum (especially A200) could significantly (p ≤ 0.05) decrease the raised weights of body and liver and the concentrations of ALP, AST, ALT, GGT, cholesterol, LDL, triglyceride, total and conjugated bilirubin, glucose, and GR and increase the concentrations of HDL, total protein, albumin, SOD, CAT, and GPx as compared to the untreated group. Also, A. eriophyllum (especially A200) decreased the degree of hepatic steatosis as compared to the untreated group. In finally, it appears that the aqueous extract of A. eriophyllum can treat the fatty liver disease in rats.


Aqueous extract Allium eriophyllum Boiss Fatty liver disease 


Compliance with ethical standards

Conflict of interest

The authors claim that there is no conflict of interest.

Ethic approval

All institutional and national guidelines for the care and use of laboratory animals were followed.


  1. Augusti KT, Narayanan A, Pillai LS, Ebrahim RS, Sivadasan R, Sindhu KR, Subha I, Abdeen S, Nair SS (2001) Beneficial effects of garlic (Allium sativum Linn) on rats fed with diets containing cholesterol and either of the oil seeds, coconuts or groundnuts. Indian J Exp Biol 39(7):660–667Google Scholar
  2. Bosisio E, Benelli C, Pirola O (1992) Effect of the flavanolignans of Silybum marianum L. on lipid peroxidation in rat liver microsomes and freshly isolated hepatocytes. Pharmacol Res 25(2):147–154CrossRefGoogle Scholar
  3. Bundy R, Walker AF, Middleton RW, Wallis C, Simpson HC (2008) Artichoke leaf extract (Cynara scolymus) reduces plasma cholesterol in otherwise healthy hypercholesterolemic adults: a randomized, double blind placebo controlled trial. Phytomedicine 15(9):668–675CrossRefGoogle Scholar
  4. Day CP (2011) Non-alcoholic fatty liver disease: a massive problem. Clin Med 11(2):176–178CrossRefGoogle Scholar
  5. Farzaei MH, Zangeneh MM, Goodarzi N, Zangeneh A (2018) Stereological assessment of nephroprotective effects of Trachyspermum ammi essential oil against carbon tetrachloride-induced nephrotoxicity in mice. Int J Morphol 36(2):750–757CrossRefGoogle Scholar
  6. Ferramosca A, Di Giacomo M, Zara V (2017) Antioxidant dietary approach in treatment of fatty liver: new insights and updates. World J Gastroenterol 23(23):4146–4157CrossRefGoogle Scholar
  7. Flora K, Hahn M, Rosen H, Benner K (1998) Milk thistle (Silybum marianum) for the therapy of liver disease. Am J Gastroenterol 93(2):139–143CrossRefGoogle Scholar
  8. Foroughi A, Zangeneh MM, Zangeneh A, Kazemi N (2016) A survey on antibacterial activities of Allium eriophyllum alcoholic extract: an ethnomedicinal plant. Iran J Public Health 45(2):32–32Google Scholar
  9. Ganz M, Csak T, Szabo G (2014) High fat diet feeding results in gender specific steatohepatitis and inflammasome activation. World J Gastroenterol 20:8525–8534CrossRefGoogle Scholar
  10. Ghashghaii A, Hashemnia M, Nikousefat Z, Zangeneh MM, Zangeneh A (2017) Wound healing potential of methanolic extract of Scrophularia striata in rats. Pharm Sci 23(4):256–263CrossRefGoogle Scholar
  11. Goodarzi N, Zangeneh MM, Zangeneh A, Najafi F, Tahvilian R (2017) Protective effects of ethanolic extract of Allium saralicum R.M. Fritsch on CCl4-induced hepatotoxicity in mice. J Rafsanjan Univ Med Sci 16(3):227–238Google Scholar
  12. Goodarzi N, Zangeneh MM, Zangeneh A (2018) The effect of ethanolic extract of Allium saralicum R.M. Fritsch on diabetic hepatopathy in male mice. Sci Res J Shahed Uni 25:21–30Google Scholar
  13. Haga Y, Kanda T, Sasaki R, Nakamura M, Nakamoto S, Yokosuka O (2015) Nonalcoholic fatty liver disease and hepatic cirrhosis: comparison with viral hepatitis-associated steatosis. World J Gastroenterol 21:12989–12995CrossRefGoogle Scholar
  14. Hagh-Nazari L, Goodarzi N, Zangeneh MM, Zangeneh A, Tahvilian R, Moradi R (2017) Stereological study of kidney in streptozotocin-induced diabetic mice treated with ethanolic extract of Stevia rebaudiana (bitter fraction). Comp Clin Pathol 26(2):455–463CrossRefGoogle Scholar
  15. Hamelian M, Zangeneh MM, Amisama A, Varmira K, Veisi H (2018) Green synthesis of silver nanoparticles using Thymus kotschyanus extract and evaluation of their antioxidant, antibacterial and cytotoxic effects. Appl Organometal Chem 32(9):e4458CrossRefGoogle Scholar
  16. Hemmati M, Zohoori E, Mehrpour O, Karamian M, Asghari S, Zarban A, Nasouti R (2015) Anti-atherogenic potential of jujube, saffron and barberry: anti-diabetic and antioxidant actions. EXCLI J 14:908–915Google Scholar
  17. Hoseinpouran M, Khaki A, Nazem H (2015) Assessment of antioxidant properties of Allium cepa on serum antioxidants and spermatogenesis after consuming tartrazine in rat. Crescent J Med Biol Sci 2(4):125–129Google Scholar
  18. Jacobs BP, Dennehy C, Ramirez G, Sapp J, Lawrence VA (2002) Milk thistle for the treatment of liver disease: a systematic review and meta-analysis. Am J Med 113(6):506–515CrossRefGoogle Scholar
  19. Janahmadi Z, Nekooeian AA, Mozafari M (2015) Hydroalcoholic extract of Allium eriophyllum leaves attenuates cardiac impairment in rats with simultaneous type 2 diabetes and renal hypertension. Res Pharm Sci 10(2):125–133Google Scholar
  20. Lee TY, Chang HH, Lo WC, Lin HC (2010) Alleviation of hepatic oxidative stress by Chinese herbal medicine Yin-Chen-Hao-tang in obese mice with steatosis. Int J Mol Med 25(6):837–844CrossRefGoogle Scholar
  21. Lee N, Lee RM, Lee CH (2017) Effects of dietary Allium hookeri root powder on the body fat deposition and biochemical parameters in guinea pigs. J Anim Res Nutr 2(2):1–6CrossRefGoogle Scholar
  22. Mohammadifar M, Behnam M, Talaei SA, Khamechian T, Mehran M, Taghizadeh M (2018) Evaluation effect of Silybum marianum, Cynara scolymus L. and Ziziphus jujube Mill. combination extract on nonalcoholic fatty liver in rats. Iranian J Endocrinol Metab 19(6):410–418Google Scholar
  23. Moradi R, Hajialiani M, Salmani S, Almasi M, Zangeneh A, Zangeneh MM (2018) Effect of aqueous extract of Allium saralicum R.M. Fritsch on fatty liver induced by high-fat diet in Wistar rats. Comp Clin Pathol.
  24. Mozafari M, Nekooeian AA, Janahmadi Z (2015) The antihypertensive effects of hydroalcoholic extract of allium eriophyllum in leaves in rats with simultaneous type 2 diabetes and renal hypertension. Int Cardiovasc Res J 9(1):34–40Google Scholar
  25. Nazni P, Vijayakumar TP, Alagianambi P, Amirthaveni M (2006) Hypoglycemic and hypolipidemic effect of Cynara scolymus among selected type 2 diabetic individuals. Pak J Nutr 5(1):147–151Google Scholar
  26. Ogunlade B, Saalu LC, Ogunmodede OS, Akunna GG, Adeeyo OA, Ajayi GO (2002) The salutary role of Allium cepa extract on the liver histology, liver oxidative status and liver marker enzymes of rabbits submitted to alcohol-induced toxicity. Am J Biochem Mol Biol 2(2):67–81Google Scholar
  27. Sabzghabaee AM, Khayam I, Kelishadi R, Ghannadi A, Soltani R, Badri S, Shirani S (2013) Effect of Zizyphus jujuba fruits on dyslipidemia in obese adolescents: a triple-masked randomized controlled clinical trial. Med Arch 67(3):156–159CrossRefGoogle Scholar
  28. Saravanan G, Ponmurugan P (2013) S-allylcysteine improves streptozotocin-induced alterations of blood glucose, liver cytochrome P450 2E1, plasma antioxidant system, and adipocytes hormones in diabetic rats. Int J Endocrinol Metab 11(4):e10927CrossRefGoogle Scholar
  29. Sayyedrostami T, Pournaghi P, Ebrahimi Vosta-Kalaeea S, Zangeneh MM (2018) Evaluation of the wound healing activity of Chenopodium botrys leaves essential oil in rats (a short-term study). J Essent Oil Bear Plants 21(1):164–174CrossRefGoogle Scholar
  30. Shaker E, Mahmoud H, Mnaa S (2010) Silymarin, the antioxidant component and Silybum marianum extracts prevent liver damage. Food Chem Toxicol 48(3):803–806CrossRefGoogle Scholar
  31. Sherkatolabbasieh H, Hagh-Nazari L, Shafiezadeh S, Goodarzi N, Zangeneh MM, Zangeneh A (2017) Ameliorative effects of the ethanolic extract of Allium saralicum R.M. Fritsch on CCl4-induced nephrotoxicity in mice: a stereological examination. Arch Biol Sci 69(3):535–543CrossRefGoogle Scholar
  32. Tamayo C, Diamond S (2007) Review of clinical trials evaluating safety and efficacy of milk thistle (Silybum marianum [L.] Gaertn.). Integr Cancer Ther 6(2):146–157CrossRefGoogle Scholar
  33. Won JY, Yoo YC, Kang EJ, Yang H, Kim GH, Seon BJ, Kim SL, Han SH, Lee SS, Lee KS (2013) Chemical components, DPPH radical scavenging activity and inhibitory effects on nitric oxide production in Allium hookeri cultivated under open field and greenhouse conditions. J Korean Soc Food Sci Nutr 42(9):1351–1356CrossRefGoogle Scholar
  34. Yao H, Qiao YJ, Zhao YL, Tao XF, Xu LN, Yin LH, Qi Y, Peng JY (2016) Herbal medicines and nonalcoholic fatty liver disease. World J Gastroenterol 22(30):6890–6905CrossRefGoogle Scholar
  35. Zangeneh MM, Goodarzi N, Zangeneh A, Tahvilian R, Najafi F (2018a) Amelioration of renal structural changes in STZ-induced diabetic mice with ethanolic extract of Allium saralicum R.M. Fritsch. Comp Clin Pathol 27(4):861–867CrossRefGoogle Scholar
  36. Zangeneh MM, Salmani S, Zangeneh A, Bahrami E, Almasi M (2018b) Antiulcer activity of aqueous extract of leaves of Mentha piperita in Wistar rats. Comp Clin Pathol.
  37. Zangeneh MM, Salmani S, Zangeneh A, Khedri R, Zarei MS (2018c) Histopathological and biochemical effects of aqueous extract of Tragopogon graminifolius on the liver tissues of Wistar rats fed with high-fat diet. Comp Clin Pathol.
  38. Zhaleh M, Sohrabi N, Zangeneh MM, Zangeneh A, Moradi R, Zhaleh H (2018) Chemical composition and antibacterial effects of essential oil of Rhus coriaria fruits in the west of Iran (Kermanshah). J Essent Oil Bear Plant 21(2):493–501CrossRefGoogle Scholar

Copyright information

© Springer-Verlag London Ltd., part of Springer Nature 2018

Authors and Affiliations

  • Samaneh Goorani
    • 1
  • Mohsen Zhaleh
    • 2
  • Majid Hajialiani
    • 3
    • 4
  • Rohallah Moradi
    • 4
  • Mohammad Kazem Koohi
    • 1
  • Khodabakhsh Rashidi
    • 5
  • Mohammad Mahdi Zangeneh
    • 4
    • 6
    Email author
  • Akram Zangeneh
    • 4
    • 6
  1. 1.Department of Toxicology, Faculty of Veterinary MedicineUniversity of TehranTehranIran
  2. 2.Department of Medical Laboratory Sciences, School of ParamedicineKermanshah University of Medical SciencesKermanshahIran
  3. 3.Depatment of Organic Chemistry, Faculty of ChemistryBu-Ali Sina UniversityHamedanIran
  4. 4.Biotechnology and Medicinal Plants Research CenterIlam University of Medical SciencesIlamIran
  5. 5.Department of Histology, Faculty of Veterinary MedicineShahid Chamran University of AhvazAhvazIran
  6. 6.Department of Clinical Science, Faculty of Veterinary MedicineRazi UniversityKermanshahIran

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