Comparative Clinical Pathology

, Volume 28, Issue 2, pp 427–434 | Cite as

Assessment of the anti-anemic effect of aqueous extract of Allium eriophyllum Boiss leaf in phenylhydrazine-treated Wistar male rats

  • Mohammad Mahdi ZangenehEmail author
  • Mohsen Zhaleh
  • Saman Salmani
  • Akram Zangeneh
  • Maryam Almasi
  • Mohammad Reza Khedri
  • Khodabakhsh Rashidi
Original Article


There are many medicinal plants in Iranian traditional medicine which are used to treat anemia. One of these plants is Allium eriophyllum Boiss. The aim of this study was to investigate the anti-anemic potential of aqueous extract of A. eriophyllum leaf. After collection of the plant, its extract was obtained using Soxhlet extractor. In this study, 60 rats were used. Induction of hemolytic anemia was done by three injections of phenylhydrazine in 50 animals. After 1 day, the rats were divided into six subgroups, including negative healthy control, untreated negative control, and four groups receiving the A. eriophyllum at 20, 40, 80, and 160 mg/kg concentrations. At the end of day 15 of treatment, the animals of all groups were sacrificed, and blood samples were drawn immediately from the animals’ hearts to analyze the biochemical and hematological parameters. The data were analyzed by SPSS-21 software. Different doses of A. eriophyllum significantly (p ≤ 0.05) reduced the raised levels of alkaline phosphatase (ALP), aspartate aminotransferase (AST), alanine aminotransferase (ALT), gamma-glutamyl transferase (GGT), urea, creatinine, ferrous, ferritin, and erythropoietin and increased the levels of body weight, white blood cell (WBC), lymphocyte, neutrophils, monocytes, platelet, red blood cell (RBC), hemoglobin (Hb), packed cell volume (PCV), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), and mean corpuscular hemoglobin concentration (MCHC) as compared to the untreated group. Seemingly, aqueous extract of A. eriophyllum can be used for the treatment of hemolytic anemia.


Allium eriophyllum Boiss Aqueous extract Hemolytic anemia Phenylhydrazine 


Funding information

The study was financially supported by the Kermanshah University of Medical Sciences.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethic approval

All institutional and national guidelines for the care and use of laboratory animals were followed.


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Copyright information

© Springer-Verlag London Ltd., part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Clinical Science, Faculty of Veterinary MedicineRazi UniversityKermanshahIran
  2. 2.Biotechnology and Medicinal Plants Research CenterIlam University of Medical SciencesIlamIran
  3. 3.Department of Medical Laboratory Sciences, School of ParamedicineKermanshah University of Medical SciencesKermanshahIran
  4. 4.Department of Histology, Faculty of Veterinary MedicineShahid Chamran University of AhvazAhvazIran

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