Comparative Clinical Pathology

, Volume 28, Issue 2, pp 419–425 | Cite as

Effects of dietary supplementation of bentonite and yeast cell wall on serum blood urea nitrogen, triglyceride, alkaline phosphatase, and calcium in high-producing dairy cattle during the transition period

  • Seyed Amin Razavi
  • Mehrdad Pourjafar
  • Ali HajimohammadiEmail author
  • Reza Valizadeh
  • Abbas Ali Naserian
  • Richard Laven
  • Kristina Ruth Mueller
Original Article


This study was conducted to evaluate the effects of dietary supplementation of endotoxins binders (Saccharomyces cerevisiae cell wall (SCW) and bentonite (BEN)) on blood urea nitrogen (BUN), triglyceride, alkaline phosphates (ALP), and calcium (Ca) in high-producing dairy cows during the transition period. Twenty-four Holstein dairy cows (n = 6, average milk production = 35.8 ± 1.6 kg/day, body weight (BW) = 567.5 ± 40.3 kg, body condition score (BCS) = 3.5 ± 0.26 out of 5) were assigned in a completely randomized design, to the following treatments: (1) basal diet (BD), (2) BD + SCW, (3) BD + BEN, and (4) BD + SCW + BEN. Blood samples were taken 4, 3, and 1 weeks before and 1and 3 weeks after parturition. Serum concentrations of BUN, triglyceride, ALP, and Ca were measured. It was observed that, BUN concentration was lower in SCW + BEN group, probably because of the better utilization of NH3-N and better stimulation for ruminal microbial protein synthesis. Triglyceride plasma concentration was lower in the control group, which might be due to decreased lipolysis and a lower pH. The treatment groups showed no effect for Ca at any time point. Serum ALP concentration was within references range. In conclusion, lower BUN concentration could be a result of better stimulation of ruminal microbial protein synthesis in the treatment groups and also due to lower triglyceride in control group, which might be result of lower pH in the rumen, we suggest that endotoxins binders (especially combined feeding of endotoxin binders) can modulate rumen function by optimizing the rumen pH.


Endotoxins binders Rumen pH Transition period Dairy cattle 


Funding information

This study was financed by PhD student project grant by School of Veterinary Medicine, Shiraz University, Shiraz, Iran. The authors would like to thank the Moghufat Malek industry for provision of cows especially Mr. Miri, Mr. Naghavi, and Mr.Ershadi.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Statement of animal right

All animals were treated in accordance with the regulations on the guidelines of the Iranian Council of Animal Care (1995), and the experiment was approved by the Institutional Animal Care Committee for Animals Used in Research.


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Copyright information

© Springer-Verlag London Ltd., part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Clinical Sciences, School of Veterinary MedicineShiraz UniversityShirazIran
  2. 2.Department of Animal Science, Faculty of AgricultureFerdowsi University of MashhadMashhadIran
  3. 3.School of Veterinary ScienceMassey UniversityPalmerston NorthNew Zealand

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