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Comparative Clinical Pathology

, Volume 28, Issue 2, pp 311–320 | Cite as

Influence of mesenchymal stem cells and royal jelly on kidney damage triggered by ischemia-reperfusion injury: comparison with ischemic preconditioning in an animal model

  • Gholamreza Didar
  • Fatemeh Delpazir
  • Maryam Kaviani
  • Negar AzarpiraEmail author
  • Lili Sepehrara
  • Padideh Ebadi
  • Farhad Koohpeyma
Original Article
  • 32 Downloads

Abstract

Renal dysfunction due to ischemia/reperfusion (I/R) injury is a common problem following reno-vascular surgery and kidney transplantation. In the present study, we investigated the protective effects of bone marrow mesenchymal stem cells (BM-MSCs), ischemic preconditioning (IPC), and royal jelly (RJ) administration on kidney I/R injury. Male Balb/c mice (30–35 g) were divided randomly into six groups including sham, I/R, I/R after IPC, I/R pre-treated with 100 mg/kg/day RJ for 20 days (I/R + RJ), treatment with 1 × 106 GFP+ BM-MSCs after I/R induction (I/R + MSCs), and I/R pre-treated with 100 mg/kg/day RJ for 20 days and treated with 1 × 106 GFP+ BM-MSCs after I/R induction (I/R + MSCs/RJ). Ten days before beginning the study, the right kidneys of all the mice were removed. I/R injury was induced by clamping the left renal artery for 45 min followed by reperfusion for 48 h. Blood serum was analyzed for blood urea nitrogen and creatinine levels. The histological patterns and the expression of FADD, BAX, BAD, BCL-2, and miR-24 were also evaluated. The BUN level was reduced in I/R + RJ and I/R + MSCs groups significantly. However, the Cr level was significantly decreased just in the I/R + RJ group. The glomerular injury and expression of apoptosis genes were significantly less in the I/R + RJ and I/R + MSCs/RJ groups. The expression of miR-24 showed reduction in all the treated groups unless IPC. Although the previous studies have shown that IPC protects kidney against I/R injury, we found out MSCs and RJ may have a higher potential to protect the kidney tissue. According to the findings, RJ may be effective to protect the kidney against I/R injury.

Keywords

Ischemia-reperfusion Mesenchymal stem cells Royal Jelly Kidney 

Notes

Acknowledgements

Our special thanks to Dr. Nasrin Shokrpour for the linguistic editing of this manuscript.

Compliance with ethical standards

Our study was compliance with ethical standards.

Conflict of interest

The authors declare that they have no conflict of interest.

Research involving human participants and/or animals

All procedures performed in studies involving animals were in accordance with the ethical standards of the institution and all applicable institutional guidelines for the care and use of animals were followed.

Informed consent

Humans are not involved in this study.

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Copyright information

© Springer-Verlag London Ltd., part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Biology, Kazerun BranchIslamic Azad UniversityKazerunIran
  2. 2.Transplant Research CenterShiraz University of Medical SciencesShirazIran
  3. 3.Department of Biochemistry, Faculty of Medicine, Kazerun BranchIslamic Azad UniversityKazerunIran
  4. 4.Shiraz Endocrinology and Metabolism Research CenterShiraz University of Medical SciencesShirazIran

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