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Comparative Clinical Pathology

, Volume 28, Issue 4, pp 949–954 | Cite as

Inorganic nanoparticles restrict viability of metastatic breast cancer cells in vitro

  • Oluyomi Stephen AdeyemiEmail author
  • David Adeiza Otohinoyi
Original Article
  • 24 Downloads

Abstract

Available cancer therapies are limited due to undesirable side effects, non-specific cellular toxicity as well as treatment failure. Therefore, there is urgent need for newer treatment strategies. In this study, we comparatively determined the in vitro anti-cancer potential of inorganic nanoparticles (NPs) in MDA-MB-231 cancer cells. Flow cytometry, confocal microscopy, and reactive oxygen species (ROS) assays were employed to probe likely mechanism of anti-cancer action of NPs. Study demonstrated dose-dependent toxicity of NPs to MDA-MB-231 cells. The NPs promoted production of ROS and might have caused early apoptotic clearance of MDA-MB-231 cells. Considered together, the findings support anti-cancer potential of inorganic NPs. Furthermore, preliminary evidence suggests that the anti-cancer potential of these NPs may be linked with capacity to cause ROS production as well as cellular apoptosis. Further studies to clearly define the mechanistic cellular actions of these nanoparticles are warranted.

Keywords

Apoptosis Cellular death Nanomedicine Toxicity 

Notes

Acknowledgements

Authors acknowledge the Department of Biochemistry and Microbiology, Rhodes University, South Africa.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

References

  1. Adeyemi O, Whiteley C (2013) Interaction of nanoparticles with arginine kinase from Trypanosoma brucei: kinetic and mechanistic evaluation. Int J Biol Macromol 62:450–456CrossRefGoogle Scholar
  2. Adeyemi O, Whiteley C (2014) Interaction of metal nanoparticles with recombinant arginine kinase from Trypanosoma brucei: thermodynamic and spectrofluorimetric evaluation. Biochim Biophys Acta 1840:701–706CrossRefGoogle Scholar
  3. American cancer society (2018) How Common Is Breast Cancer? https://www.cancer.org/cancer/breast-cancer/about/how-common-is-breast-cancer.html. Accessed 4 Mar 2018
  4. Asharani P, lianwu Y, Gong Z, Valiyaveettil S (2010) Comparison of the toxicity of silver, gold and platinum nanoparticles in developing zebrafish embryos. Nanotoxicol 5:43–54CrossRefGoogle Scholar
  5. Azizi M, Ghourchian H, Yazdian F, Bagherifam S, Bekhradnia S, Nyström B (2017) Anti-cancerous effect of albumin coated silver nanoparticles on MDA-MB-231 human breast cancer cell line. Sci Rep 7:5178CrossRefGoogle Scholar
  6. Azizi M, Ghourchian H, Yazdian F, Alizadehzeinabad H (2018) Albumin coated cadmium nanoparticles as chemotherapeutic agent against MDA-MB-231 human breast cancer cell line. Artif Cells Nanomed Biotechnol 46:1–11CrossRefGoogle Scholar
  7. Bhattacharya R, Mukherjee P (2008) Biological properties of “naked” metal nanoparticles. Adv Drug Deliv Rev 60:1289–1306CrossRefGoogle Scholar
  8. Centers for Disease Control and Prevention (2018) Breast cancer in young women https://www.cdc.gov/cancer/breast/young_women/bringyourbrave/pdf/breastcanceryoungwomenfactsheet.pdf. Accessed 3 Mar 2018
  9. Duangmano S, Sae-lim P, Suksamrarn A, Domann F, Patmasiriwat P (2012) Cucurbitacin B inhibits human breast cancer cell proliferation through disruption of microtubule polymerization and nucleophosmin/B23 translocation. BMC Complement Altern Med 12:185CrossRefGoogle Scholar
  10. Gurunathan S, Han J, Eppakayala V, Jeyaraj M, Kim J (2013) Cytotoxicity of biologically synthesized silver nanoparticles in MDA-MB-231 human breast cancer cells. Biomed Res Int 2013:1–10CrossRefGoogle Scholar
  11. Krishnaraj C, Muthukumaran P, Ramachandran R, Balakumaran M, Kalaichelvan P (2014) Acalypha Indica Linn: biogenic synthesis of silver and gold nanoparticles and their cytotoxic effects against MDA-MB-231, human breast cancer cells. Biotechnol Rep 4:42–49CrossRefGoogle Scholar
  12. Kumar V, Yadav S (2009) Plant-mediated synthesis of silver and gold nanoparticles and their applications. J Chem Technol Biotechnol 84:151–157CrossRefGoogle Scholar
  13. Meyer J (2017) Silver nanoparticles are in general more toxic to C. elegans than tested gold, copper, iron, titanium dioxide, zinc oxide, cerium oxide, and carbon-based nanoparticles http://wbg.wormbook.org/2017/01/17/silver-nanoparticles-are-in-general-more-toxic-to-c-elegans-than-tested-gold-copper-iron-titanium-dioxide-zinc-oxide-cerium-oxide-and-carbon-based-nanoparticles/. Accessed 4 Mar 2018
  14. Moses SL, Edwards VM, Brantley E (2016) Cytotoxicity in MCF-7 and MDA-MB-231 breast cancer cells, without harming MCF-10A healthy cells. J Nanomed Nanotechnol 7:369Google Scholar
  15. Shrivastava R, Kushwaha P, Bhutia Y, Flora S (2014) (2014) Oxidative stress following exposure to silver and gold nanoparticles in mice. Toxicol Ind Health 32:1391–1404CrossRefGoogle Scholar
  16. Thoidingjam S, Tiku A (2017) New developments in breast cancer therapy: role of iron oxide nanoparticles. Adv Nat Sci Nanosci Nanotechnol 8:023002CrossRefGoogle Scholar
  17. Toyokuni S, Okamoto K, Yodoi J, Hiai H (1995) Persistent oxidative stress in cancer. FEBS Lett 358:1–3CrossRefGoogle Scholar
  18. Wang W, Zhang L, Chen T, Guo W, Bao X, Wang D, Ren B, Wang H, Li Y, Wang Y, Chen S, Tang B, Yang Q, Chen C (2017) Anticancer effects of resveratrol-loaded solid lipid nanoparticles on human breast cancer cells. Molecules 22:1814CrossRefGoogle Scholar
  19. Warleta F, Quesada C, Campos M, Allouche Y, Beltrán G, Gaforio J (2011) Hydroxytyrosol protects against oxidative DNA damage in human breast cells. Nutrients 3:839–857CrossRefGoogle Scholar
  20. Yuan Y, Peng Q, Gurunathan S (2017) Combination of palladium nanoparticles and tubastatin-A potentiates apoptosis in human breast cancer cells: a novel therapeutic approach for cancer. Int J Nanomedicine 12:6503–6520CrossRefGoogle Scholar

Copyright information

© Springer-Verlag London Ltd., part of Springer Nature 2018

Authors and Affiliations

  1. 1.Medicinal Biochemistry, Nanomedicine & Toxicology Laboratory, Department of BiochemistryLandmark UniversityOmu-AranNigeria
  2. 2.All Saints University, School of MedicineRoseauCommonwealth of Dominica

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