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Comparative Clinical Pathology

, Volume 28, Issue 2, pp 297–303 | Cite as

Evaluation of protective effect of aqueous extract of Phyllanthus emblica fruit on duodenum mucosa in experimentally induced diarrhea in albino Wistar rats

  • D. K. SharmaEmail author
  • V. K. Gupta
  • D. B. Mondal
Original Article
  • 36 Downloads

Abstract

Phyllanthus emblica fruit is an excellent source of antioxidants which is widely used for different gastric ailments in Ayurveda and traditional herbal medicine. Thus, the aim of this study was to investigate the antioxidative and protective role of aqueous extract of Phyllanthus emblica fruit (AEPEF) on intestinal mucosa against castor oil-induced diarrhea. Rats were randomly divided into five groups (N = 6). After acclimatization of 7 days, group I (control) and group II (loperamide) were given distilled water @ 20 ml/kg body weight (BW) orally whereas rats of group III, group IV, and group V received AEPEF @ 200 mg, 400 mg, and 600 mg/kg BW respectively, daily for 14 days. On the 15th day, diarrhea was induced with 1 ml castor oil to each rat orally. Onset time of diarrhea and percentage inhibition of defecation revealed significantly (p < 0.05) higher onset time and higher percentage inhibition of defecation respectively in group II, group V, and group IV as compared to group I. In groups IV and V, lipid peroxidation was significantly low whereas superoxide dismutase, catalase, and glutathione peroxidase found to be significantly increased as compared to groups I and II. Duodenal histopathology of group IV and group V revealed no change in mucosal integrity whereas degenerative changes like stunting, infiltration of polymorphs, and villous atrophy were observed in group I and group II. The study concludes that prior treatment with AEPEF delays the induction of diarrhea and protects intestinal mucosa from the deleterious effect of castor oil-induced oxidative damage.

Keywords

Antioxidant Diarrhea Intestinal mucosal injury Phyllanthus emblica 

Notes

Acknowledgments

The authors acknowledge the Director, ICAR-Indian Veterinary Research Institute, Izatnagar, for providing necessary facilities and funding for this research work.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical clearance

Prior ethical clearance for experimentation was approved by the Institutional Animal Ethical Committee (IAEC), ICAR-IVRI, Izatnagar, Bareilly. All the experimentation was conducted as per the guidelines laid down by the Committee for the Purpose of Control and Supervision of Experiments on Animals (CPCSEA).

References

  1. Ahmed AS, Elgorashi EE, Moodley N, McGaw LJ, Naidoo V, Eloff JN (2012) The antimicrobial, antioxidative, anti-inflammatory activity and cytotoxicity of different fractions of four South African Bauhinia species used traditionally to treat diarrhea. J Ethnopharmacol 143(3):826–839CrossRefGoogle Scholar
  2. Awe EO, Kolawole SO, Wakeel KO, Abiodun OO (2011) Antidiarrheal activity of Pyrenacantha staudtii Engl. (Iccacinaceae) aqueous leaf extract in rodents. J Ethnopharmacol 137(1):148–153CrossRefGoogle Scholar
  3. Awouters F, Niemegeers CJE, Lenaerts FM, Janssen PAJ (1978) Delay of castor oil-diarrhea in rats: a new way to evaluate inhibitors of prostaglandin biosynthesis. J Pharm Pharmacol 30(1):41–45CrossRefGoogle Scholar
  4. Baldi F, Bianco MA, Nardone G, Pilotto A, Zamparo E (2009) Focusonacute diarrhoeal disease. World J Gastroenterol 15:3341–3348CrossRefGoogle Scholar
  5. Baliga MS, Dsouza JJ (2011) Amla (Emblica officinalis Gaertn), a wonder berry in the treatment and prevention of cancer. Eur J Cancer Prev 20(3):225–239CrossRefGoogle Scholar
  6. Bandyopadhyay SK, Pakrashi SC, Pakrashi A (2000) The role of antioxidant activity of Phyllanthus emblica fruits on prevention from indomethacin induced gastric ulcer. J Ethnopharmacol 70(2):171–176CrossRefGoogle Scholar
  7. Cohen G, Dembiec D, Marcus J (1970) Measurement of catalase activity in tissue extracts. Anal Biochem 34(1):30–38CrossRefGoogle Scholar
  8. Coles EH (1980) Veterinary clinical pathology, 3rd edn. W.R. Sounders company, LondonGoogle Scholar
  9. Culling CFA (1963) Handbook of histological techniques (including museum techniques), 2nd edn. Butterworth, LondonGoogle Scholar
  10. Gilani AH (2005) Trends in ethnopharmacology. J Ethnopharmacol 100(1):43–49CrossRefGoogle Scholar
  11. Haldane J (1901) The colorimetric determination of hemoglobin. J Physiol 26(6):497–504CrossRefGoogle Scholar
  12. Harborne JB (1998) Phytochemical methods: a guide to modern techniques of plant analysis, third edn. Chapman and Hall, LondonGoogle Scholar
  13. Heinrich M, Heneka B, Ankli A, Rimpler H, Sticher O, Kostiza T (2005) Spasmolytic and antidiarrheal properties of the Yucatec Mayan medicinal plant Casimiroatetrameria. J Pharm Pharmacol 57(9):1081–1086CrossRefGoogle Scholar
  14. Huang D, Ou B, Prior RL (2005) The chemistry behind antioxidant capacity assays. J Agric Food Chem 53(6):1841–1856CrossRefGoogle Scholar
  15. Jose JK, Kuttan R (2000) Hepatoprotective activity of Emblica officinalis and Chyavanaprash. J Ethnopharmacol 72(1):135–140CrossRefGoogle Scholar
  16. Liu X, Zhao M, Wang J, Yang B, Jiang Y (2008) Antioxidant activity of methanolic extract of emblica fruit (Phyllanthus emblica L.) from six regions in China. J Food Compos Anal 21(3):219–228CrossRefGoogle Scholar
  17. Lowry OH, Rosenbrough NJ, Farr AL, Randeael RJ (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193(1):265–275Google Scholar
  18. Luo W, Zhao M, Yang B, Ren J, Shen G, Rao G (2011) Antioxidant and antiproliferative capacities of phenolics purified from Phyllanthus emblica L. fruit. Food Chem 126(1):277–282CrossRefGoogle Scholar
  19. Manonmani S, William S, Subramanian S, Govindasamy S (1991) Biochemical studies on the antidiarrheal effects of Cauvery-100, an ayurvedic formulation, in rats. Biochem Int 24(4):701–708Google Scholar
  20. Masayasu M, Hiroshi Y (1979) A simplified assay method of superoxide dismutase activity for clinical use. Clin Chim Acta 92(3):337–342CrossRefGoogle Scholar
  21. Mascolo N, Izzo AA, Barbato F, Capasso F (1993) Inhibitors of nitric oxide synthetase prevent castor-oil-induced diarrhea in the rat. Br J Pharmacol 108(4):861–864CrossRefGoogle Scholar
  22. Mehmood MH, Siddiqi HS, Gilani AH (2011) The antidiarrheal and spasmolytic activities of Phyllanthus emblica are mediated through dual blockade of muscarinic receptors and Ca2+ channels. J Ethnopharmacol 133:856–865CrossRefGoogle Scholar
  23. Ohkawa H, Ohishi N, Yagi K (1979) Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 95:351–358CrossRefGoogle Scholar
  24. Okeke MI, Iroegbu CU, Okoli AS, Esimone CO (2001) Evaluation of extracts of the root of Landolphiaowerrience for antibacterial activity. J Ethnopharmacol 78(2):119–127CrossRefGoogle Scholar
  25. Panchabhai TS, Ambarkhane SV, Joshi AS, Samant BD, Rege NN (2008) Protective effect of Tinosporacordifolia, Phyllanthus emblica and their combination against antitubercular drugs induced hepatic damage: an experimental study. Phytother Res 22(5):646–650CrossRefGoogle Scholar
  26. Perianayagam JB, Narayanan S, Gnanasekar G, Pandurangan A, Raja S, Rajagopal K, Rajesh R, Vijayarajkumar P, Vijayakumar SG (2005) Evaluation of antidiarrheal potential of Emblica officinalis. Pharm Biol 43(4):373–377CrossRefGoogle Scholar
  27. Petri WA, Miller M, Binder HJ, Levine MM, Dillingham R, Guerrant RL (2008) Enteric infections, diarrhea, and their impact on function and development. J Clin Invest 118(4):1277–1290CrossRefGoogle Scholar
  28. Raghu V, Platel K, Srinivasan K (2007) Comparison of ascorbic acid content of Emblica officinalis fruits determined by different analytical methods. J Food Compos Anal 20(6):529–533CrossRefGoogle Scholar
  29. Reddy VD, Padmavathi P, Varadacharyulu NC (2009) Emblica officinalis protects against alcohol-induced liver mitochondrial dysfunction in rats. J Med Food 12(2):327–333CrossRefGoogle Scholar
  30. Sedlak J, Lindsay RH (1968) Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman’s reagent. Anal Biochem 25:192–205CrossRefGoogle Scholar
  31. Sharma DK, Gupta VK, Kumar S, Joshi V, Mandal RSK, Prakash AB, Singh M (2015) Evaluation of antidiarrheal activity of ethanolic extract of Holarrhena antidysenterica seeds in rats. Vet World 8:1392–1995CrossRefGoogle Scholar
  32. Shoba FG, Thomas M (2001) Study of antidiarrheal activity of four medicinal plants in castor-oil induced diarrhoea. J Ethnopharmacol 76:73–76CrossRefGoogle Scholar
  33. Thilakchand KR, Mathai RT, Simon P, Ravi RT, Baliga-Rao MP, Baliga MS (2013) Hepatoprotective properties of the Indian gooseberry (Emblica officinalis Gaertn): a review. Food Funct 4(10):1431–1441CrossRefGoogle Scholar

Copyright information

© Springer-Verlag London Ltd., part of Springer Nature 2018

Authors and Affiliations

  1. 1.ICAR-Indian Veterinary Research InstituteBareillyIndia

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