Comparative Clinical Pathology

, Volume 28, Issue 4, pp 927–936 | Cite as

Medicinal properties of Clerodendrum glaburum E may leaf extracts: phytochemical constituents, antioxidant, cytotoxicity, and carbohydrate-metabolizing enzyme inhibitory potentials

  • Akintayo Lanre OgundajoEmail author
  • Anofi Omotayo Tom Ashafa
Original Article


The phytochemical constituents, antioxidant, and cytotoxicity as well as the inhibitory potentials of Clerodendrum glaburum on key carbohydrate metabolizing enzymes were investigated. The plant sample was extracted separately with hexane, EtOAc, MeOH, and water. The phytochemical analysis and antioxidant assays of the extracts were achieved using standard procedures; the antidiabetic capability of the extracts against the actions of α-amylase and α-glucosidase was examined while their cytotoxicity was tested against Vero cells. Highest quantity of phenol (65.97 mg gallic acid g−1), flavonoid (47.02 mg quercetin g−1), and flavanol (173.74 mg catechin g−1) were observed in MeOH extract. Also, MeOH extract had the most potent ability (p < 0.05) to scavenge ABTS (0.05 mg/mL), DPPH (0.17 mg/mL), and superoxide anion (0.36 mg/mL) than other extracts and standards. Stronger inhibition (p < 0.05) against metal chelation (26.41 mg/mL) and FRAP (5.92 mg/mL) were observed in water extract compared to other extracts; it also competes favorably with the standard. EtOAc extract displayed best scavenging potentials (p < 0.05) against hydroxyl radical than other extracts. Methanol (0.71 mg/mL) and aqueous (0.19 mg/mL) extracts displayed more potent inhibition against the actions of α-amylase and α-glucosidase respectively compared to acarbose and other extracts. Hexane extract displayed better antidiabetic activities as revealed by its moderate α-amylase (2.54 mg/mL) and potent α-glucosidase (0.53 mg/mL) inhibitions compared to acarbose. The hexane, methanol, and aqueous extracts were non-toxic against Vero cells, with LC50 of 0.11, 0.23, and 0.52 mg/mL respectively. C. glaburum leaves contain active phytochemicals that can be beneficial in managing diabetes and other oxidative stress-induced disorders.


Clerodendrum glaburum Antioxidants Antidiabetics Phytochemicals Cytotoxicity 



We acknowledge the technical assistance of Dr. Lateef Ariyo Adeniran.


This study was funded by Directorate Research Development, University of Free state, South Africa (Entity No. 2114 B5004).

Compliance with ethical standards

Conflict of interest

Akintayo Lanre Ogundajo was awarded Postdoctoral fellowship from Directorate Research Development, University of Free state, South Africa for (Entity No. 2114 B5004) tenable at the Phytomedicine and Phytopharmacology Research Unit of the Department of Plant Sciences, University of the Free State (UFS), Qwaqwa Campus, Phuthaditjhaba, South Africa.

Ethical approval

This article does not contain any studies with animals performed by any of the authors.


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Copyright information

© Springer-Verlag London Ltd., part of Springer Nature 2018

Authors and Affiliations

  1. 1.Phytomedicine and Phytopharmacology Research Group, Department of Plant SciencesUniversity of the Free StatePhuthaditjhabaSouth Africa

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