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Comparative Clinical Pathology

, Volume 28, Issue 1, pp 253–258 | Cite as

Post-prandial lipemia and glycemia in dogs fed with industrialized pet food

  • N. L. T. Silva
  • N. C. M. Bonatto
  • P. L. Oliveira
  • G. C. Vieira
  • B. P. Floriano
  • L. D. Barros
  • M. R. M. Bosculo
  • B. F. M. AlmeidaEmail author
Original Article
  • 31 Downloads

Abstract

Although lipemia is known to interfere with spectrophotometric analyses in dogs and other species, the postprandial profile of dogs fed with industrialized pet food is yet to be determined. Analytical errors, especially during postprandial peak lipemia, are likely to occur due to an increase in blood serum turbidity caused by lipoproteins. Therefore, the aim of this study was to determine the time to postprandial peak triglyceride and glucose levels in healthy dogs fed with commercial feed, their return to baseline levels, and whether feeding could alter lipid and glycemic profiles in dogs. Twenty healthy dogs fasted for 12 h were fed with commercial feed. Blood samples obtained hourly and biochemical analyses were performed through spectrophotometry. VLDL and LDL were calculated using Friedewald’s equation. Variables were tested for normality and compared through repeated measures ANOVA or Friedman’s test. Peak hypertriglyceridemia and glucose happened 2–5 and 1–3 h following feeding, respectively. Baseline levels were again found at 6 h for triglycerides and 4 for glucose. Total cholesterol and LDL cholesterol levels were not changed by commercial feed. In conclusion, the commercial feed used in this study alters glycemic and lipid profiles of some analytes of healthy dogs. Fasting can be reduced for biochemical determination of glucose, while no fasting period is necessary for total and HDL cholesterol determination in dogs.

Keywords

Canine Glucose Triglyceride Cholesterol Fasting 

Notes

Acknowledgments

The authors are grateful to the São Paulo Research Foundation for the scholarship granted to the first author (FAPESP no. 2017/02641-4).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflicts of interest.

Ethical approval

All applicable international, national, and/or institutional guidelines for the care and use of dogs were strictly followed. This article does not contain any studies with human participants performed by any of the authors.

Supplementary material

580_2018_2824_MOESM1_ESM.docx (16 kb)
ESM 1 (DOCX 15 kb)

References

  1. Alleman AR (1990) The effects of hemolysis and lipemia on serum biochemical constituents. Vet Med 85:1272–1284Google Scholar
  2. Bauer JE (1996) Comparative lipid and lipoprotein metabolism. Vet Clin Pathol 25:49–56CrossRefGoogle Scholar
  3. Bauer JE (2004) Lipoprotein-mediated transport of dietary and synthesized lipids and lipid abnormalities of dogs and cats. J Am Vet Med Assoc 224:668–675CrossRefGoogle Scholar
  4. Bertolucci C, Fazio F, Piccione G (2008) Daily rhythms of serum lipids in dogs: influences of lighting and fasting cycles. Comp Med 58:485–489Google Scholar
  5. Downs LG, Crispin SM, LeGrande-Defretin V et al (1997) The influence of lifestyle and diet on the lipoprotein profile of border collies. Res Vet Sci 63:35–42CrossRefGoogle Scholar
  6. Elliott KF, Rand JS, Fleeman LM et al (2011) Use of a meal challenge test to estimate peak postprandial triglyceride concentrations in dogs. Am J Vet Res 72:161–168.  https://doi.org/10.2460/ajvr.72.2.161 CrossRefGoogle Scholar
  7. Ferreira PA, Capella SO, Theodoro SS et al (2015) Serum lipid profile of spayed and non-spayed female dogs associated with the body condition score. Ciência Anim 16:262–267CrossRefGoogle Scholar
  8. Ford RB (1996) Clinical management of lipemic patients. Compend Contin Educ Pract Vet 18:1053–1065Google Scholar
  9. Friedewald WT, Levy RI, Fredrickson DS (1972) Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem 18:499–502Google Scholar
  10. Garvey WT, Kwon S, Zheng D et al (2003) Effects of insulin resistance and type 2 diabetes on lipoprotein subclass particle size and concentration determined by nuclear magnetic resonance. Diabetes 52:453–462CrossRefGoogle Scholar
  11. Glick MR, Ryder KW, Glick SJ, Woods JR (1989) Unreliable visual estimation of the incidence and amount of turbidity, hemolysis, and icterus in serum from hospitalized patients. Clin Chem 35:837–839Google Scholar
  12. González F, Carvalho V, Möller VA, Duarte FR (2001) Perfil bioquímico sangüíneo de cães e gatos na cidade de Porto Alegre, Rio Grande do Sul, Brasil. Arq da Fac Veterinária UFRGS 29:1–6Google Scholar
  13. Jacobs RM, Lumsden JH, Grift E (1992) Effects of bilirubinemia, hemolysis, and lipemia on clinical chemistry analytes in bovine, canine, equine, and feline sera. Can Vet J 33:605–608Google Scholar
  14. Kaneko JJ, Harvey JW, Bruss ML (2008) Clinical biochemistry of domestic animals, 6th edn. Academic Press, LondonGoogle Scholar
  15. Kazmierczak SC (2013) Hemolysis, lipemia, and high bilirubin: Effect on laboratory tests. In: Dasgupta A, Sepulveda JL (eds) . Elsevier, Amsterdam, pp 53–62Google Scholar
  16. Klein BG (2013) Cunningham’s Textbook of Veterinary Physiology, 6th edn. Elsevier Health Sciences, Saint LouisGoogle Scholar
  17. Laflamme D (1997) Development and validation of a body condition score system for dogs. Canine Pract 22:10–15Google Scholar
  18. Martinez-Subiela S, Ceron J (2005) Evaluation of acute phase protein indexes in dogs with leishmaniasis at diagnosis, during and after short-term treatment. Vet Med (Praha) 50:39–46CrossRefGoogle Scholar
  19. Moreno P, Ginel PJ (1999) Effects of haemolysis, lipaemia and bilirubinaemia on prothrombin time, activated partial thromboplastin time and thrombin time in plasma samples from healthy dogs. Res Vet Sci 67:273–276.  https://doi.org/10.1053/rvsc.1999.0321 CrossRefGoogle Scholar
  20. Mori N, Lee P, Kondo K et al (2011) Potential use of cholesterol lipoprotein profile to confirm obesity status in dogs. Vet Res Commun 35:223–235.  https://doi.org/10.1007/s11259-011-9466-x CrossRefGoogle Scholar
  21. Nigam PK (2011) Serum lipid profile: Fasting or non-fasting? Indian J Clin Biochem 26:96–97.  https://doi.org/10.1007/s12291-010-0095-x CrossRefGoogle Scholar
  22. Osorio JH (2009) The variability in the canine lipid profile values and its possible relationship with the measurement method used. Vet ir Zootech 3:70–77Google Scholar
  23. Pasquini A, Luchetti E, Cardini G (2008) Plasma lipoprotein concentrations in the dog: the effects of gender, age, breed and diet. J Anim Physiol Anim Nutr (Berl) 92:718–722.  https://doi.org/10.1111/j.1439-0396.2007.00771.x CrossRefGoogle Scholar
  24. Radin MJ (2012) Laboratory evaluation of lipids. In: Thrall MA, Weiser G, Allison RW, Campbell TW (eds) Veterinary Hematology and Clinical Chemistry, 2nd edn. Wiley, Ames, pp 480–496Google Scholar
  25. Silva Júnior JW, Borges FMO, Murgas LDS et al (2004) Digestibiliade de dietas com diferentes fontes de carboidratos e sua influência na glicemia e insulinemia em cães. Ciência e Agrotecnologia Lavras 29:436–443CrossRefGoogle Scholar
  26. Simundic AM, Nikolac N, Vukasovic I, Vrkic N (2010) The prevalence of preanalytical errors in a Croatian ISO 15189 accredited laboratory. Clin Chem Lab Med 48:1009–1014.  https://doi.org/10.1515/CCLM.2010.221 Google Scholar
  27. Thrall MA, Weiser G, Allison RW, Campbell TW (2012) Veterinary Hematology and Clinical Chemistry, 2nd edn. Wiley, AmesGoogle Scholar
  28. Whitney MS (1992) Evaluation of hyperlipidemias in dogs and cats. Semin Vet Med Surg 7:292–300Google Scholar
  29. Xenoulis PG, Steiner JM (2010) Lipid metabolism and hyperlipidemia in dogs. Vet J 183:12–21.  https://doi.org/10.1016/j.tvjl.2008.10.011 CrossRefGoogle Scholar
  30. Xenoulis PG, Steiner JM (2015) Canine hyperlipidaemia. J Small Anim Pract 56:595–605.  https://doi.org/10.1111/jsap.12396 CrossRefGoogle Scholar

Copyright information

© Springer-Verlag London Ltd., part of Springer Nature 2018

Authors and Affiliations

  1. 1.Curso de Medicina VeterináriaFaculdades Integradas de Ourinhos (FIO)OurinhosBrazil
  2. 2.Programa de Aprimoramento em Medicina VeterináriaFaculdades Integradas de Ourinhos (FIO)OurinhosBrazil
  3. 3.Docente, Curso de Medicina VeterináriaFaculdades Integradas de Ourinhos (FIO)OurinhosBrazil
  4. 4.FarmacêuticaHospital Veterinário Roque Quagliato, Faculdades Integradas de Ourinhos (FIO)OurinhosBrazil

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