Comparative Clinical Pathology

, Volume 28, Issue 1, pp 233–239 | Cite as

Variations of immunoglobulin A in serum, tracheal, and intestinal tissues in chickens following inoculation of two strains of Newcastle disease vaccines

  • Keramat AsasiEmail author
  • Saeed Mokhayeri
  • Hassan Nili
  • Hadi Rohollahzadeh Lamesu
  • Amir Hossein Asl Najjari
Original Article


To elucidate secretory immunoglobulin A (IgA) response as a part of mucosal immunity, it is decided to compare the effectiveness of intraocular administration of Hitchner B1 and thermostable I-2 vaccines in broiler chickens challenged with viscerotropic velogenic Newcastle disease virus (vvNDV) (Herts33). For this purpose, 301-day-old chicks were randomly divided in to the four groups. Groups 1 and 2 were inoculated with I-2 and HB1 vaccines twice via intraocular route with 1-week intervals, and groups 3 and 4 were considered positive and negative controls and birds were challenged 2 weeks after the second inoculation. Protection rate in I-2 and HB1 vaccine groups was 100% and 97% respectively with no significant differences (P > 0.05). All vaccinated groups showed elevation in ND HI antibody titer after vaccination. There were no significant differences (P > 0.05) in ND HI antibody between two vaccinated groups. First levels of detectable total IgA in a serum sample were observed 3 dpv1. No significant differences (P > 0.05) were observed between two vaccinated groups in IgA antibody titer in sera. The vaccinated groups were evaluated by IHC for detection IgA in the trachea and intestine. There were some differences between the vaccinated groups but these differences were not significant. Experimental studies have shown that both vaccines induce antibody production in mucosal surface and circulatory system and thermostable I-2 vaccine could be successfully used in the broiler chicken as effective as HB1 vaccine, so I-2 vaccine could be a suitable heat resistance candidate to reduce mortality in the poultry industry faced to vvND.


Immunoglobulin A Newcastle disease Velogenic NDV Thermostable I-2 vaccine Hitchner B1 vaccine Broiler chicken 



Days post vaccination


Days post challenge


Days post first vaccination


Days post second vaccination


Immunoglobulin A




Funding Information

This research was financially supported by a grant of Shiraz University Research Council.

Compliance with ethical standards

All experiments were conducted after institutional approval of the animal use committee of Shiraz University that follows the recommendations stated in the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health.

Conflict of interests

The authors declare that they have no conflict of interest.


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Copyright information

© Springer-Verlag London Ltd., part of Springer Nature 2018

Authors and Affiliations

  1. 1.Poultry Diseases Research Center, Avian Medicine Section, Department of Clinical Sciences, School of Veterinary MedicineShiraz UniversityShirazIran
  2. 2.Group of Biological Sciences, Faculty of SciencesUniversity of IsfahanIsfahanIran

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