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Comparative Clinical Pathology

, Volume 28, Issue 1, pp 233–239 | Cite as

Variations of immunoglobulin A in serum, tracheal, and intestinal tissues in chickens following inoculation of two strains of Newcastle disease vaccines

  • Keramat AsasiEmail author
  • Saeed Mokhayeri
  • Hassan Nili
  • Hadi Rohollahzadeh Lamesu
  • Amir Hossein Asl Najjari
Original Article
  • 15 Downloads

Abstract

To elucidate secretory immunoglobulin A (IgA) response as a part of mucosal immunity, it is decided to compare the effectiveness of intraocular administration of Hitchner B1 and thermostable I-2 vaccines in broiler chickens challenged with viscerotropic velogenic Newcastle disease virus (vvNDV) (Herts33). For this purpose, 301-day-old chicks were randomly divided in to the four groups. Groups 1 and 2 were inoculated with I-2 and HB1 vaccines twice via intraocular route with 1-week intervals, and groups 3 and 4 were considered positive and negative controls and birds were challenged 2 weeks after the second inoculation. Protection rate in I-2 and HB1 vaccine groups was 100% and 97% respectively with no significant differences (P > 0.05). All vaccinated groups showed elevation in ND HI antibody titer after vaccination. There were no significant differences (P > 0.05) in ND HI antibody between two vaccinated groups. First levels of detectable total IgA in a serum sample were observed 3 dpv1. No significant differences (P > 0.05) were observed between two vaccinated groups in IgA antibody titer in sera. The vaccinated groups were evaluated by IHC for detection IgA in the trachea and intestine. There were some differences between the vaccinated groups but these differences were not significant. Experimental studies have shown that both vaccines induce antibody production in mucosal surface and circulatory system and thermostable I-2 vaccine could be successfully used in the broiler chicken as effective as HB1 vaccine, so I-2 vaccine could be a suitable heat resistance candidate to reduce mortality in the poultry industry faced to vvND.

Keywords

Immunoglobulin A Newcastle disease Velogenic NDV Thermostable I-2 vaccine Hitchner B1 vaccine Broiler chicken 

Abbreviations

dpv

Days post vaccination

dpc

Days post challenge

dpv1

Days post first vaccination

dpv2

Days post second vaccination

IgA

Immunoglobulin A

IHC

Immunohistochemistry

Notes

Funding Information

This research was financially supported by a grant of Shiraz University Research Council.

Compliance with ethical standards

All experiments were conducted after institutional approval of the animal use committee of Shiraz University that follows the recommendations stated in the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health.

Conflict of interests

The authors declare that they have no conflict of interest.

References

  1. Alders R, Spradbrow PB (2001) Controlling Newcastle disease in village chickens: a field manual. ACIAR Monograph 82. 112ppGoogle Scholar
  2. Alexander DJ, Aldous EW, Fuller CM (2012) The long view: a selective review of 40 years of Newcastle disease research. Avian Pathol 41:329–335.  https://doi.org/10.1080/03079457.2012.697991 CrossRefGoogle Scholar
  3. Al-Garib SO, Gielkens ALJ, Gruys E, Hartog L, Koch G (2003) Immunoglobulin class distribution of systemic and mucosal antibody responses to Newcastle disease in chickens. Avian Dis 47:32–40. https://doi.org/10.1637/0005-2086(2003)047[0032:ICDOSA]2.0.CO;2Google Scholar
  4. Bensink Z, Spradbrow P (1999) Newcastle disease virus strain I-2 a prospective thermostable vaccine for use in developing countries. Vet Microbiol 68:131–139.  https://doi.org/10.1016/S0378-1135(99)00069-3 CrossRefGoogle Scholar
  5. Habibi H, Nili H, Asasi K, Nazifi S, Mosleh N, Firouzi S (2013) Acute phase responses in village chicken challenged by a wild Newcastle disease virus isolate (JF820294.1.). Online J Vet Res 17:571–577Google Scholar
  6. Habibi H, Nili H, Asasi K, Mosleh N, Firouzi S, Mohammadi M (2015) Efficacy and transmissibility of Newcastle disease I-2 vaccine strain against a field isolate of virulent ND virus (JF820294.1) in village chicken. Trop Anim Health Prod 47(1):73–78.  https://doi.org/10.1007/s11250-014-0687-1
  7. Hassanzadeh M, BozorgmehriFard MH (2004) A serological study of Newcastle disease in pre- and post-vaccinated village chickens in north of Iran. Int J Poult Sci 5:70–75Google Scholar
  8. Hsiang-jung TSA, Dih-Fa LIN (1998) Evaluation of the protection efficacy of newcastle disease vaccination programs. Journal of the Republic of China Veterinary Society, 1999Google Scholar
  9. Kagnoff MF (1996) Mucosal immunology: new frontiers. Immunol Today 17:57–59.  https://doi.org/10.1016/0167-5699(96)80579-2 CrossRefGoogle Scholar
  10. Kapczynski DR, Afonso CL, Miller PJ (2013) Immune responses of poultry to Newcastle disease virus. Dev Comp Immunol 41:447–453.  https://doi.org/10.1016/j.dci.2013.04.012 CrossRefGoogle Scholar
  11. Mahy BWJ, Kangro H (1996) Hemagglutination-inhibition. In: Virology methods manual. Academic Press Ltd., London, pp 114–116Google Scholar
  12. Martinez JCS, Chou WK, Berghman LR, Carey JB (2018) Evaluation of the effect of live LaSota Newcastle disease virus vaccine as primary immunization on immune development in broilers. Poult Sci 97:455–462.  https://doi.org/10.3382/ps/pex339 CrossRefGoogle Scholar
  13. Musa U, Abdu PA, Mera UM, Emmenna PE, Ahmed MS (2010) Vaccination with Newcastle disease vaccines strain I2 and LaSota in commercial and local chickens in Plateau State Nigeria. Niger Vet J 31:46–55.  https://doi.org/10.4314/nvj.v31i1.68940 Google Scholar
  14. Nasser M, Lohr J, Mebratu G, Zessin K-H, Baumann M, Ademe Z (2000) Oral Newcastle disease vaccination trials in Ethiopia. Avian Pathol 29:27–34.  https://doi.org/10.1080/03079450094243 CrossRefGoogle Scholar
  15. Perozo F, Villegas P, Dolz R, Afonso CL, Purvis LB (2008) The VG/GA strain of Newcastle disease virus: mucosal immunity, protection against lethal challenge and molecular analysis. Avian Pathol 37:237–245.  https://doi.org/10.1080/03079450802043734 CrossRefGoogle Scholar
  16. Quan S, Yunlian S, Ruiping S, Taozhen J, Decheng W, Ye D, Jun Y (2013) Detection of intestinal intraepithelial lymphocytes, goblet cells and secretory IgA in the intestinal mucosa during Newcastle disease virus infection. Avian Pathol 42:541–545.  https://doi.org/10.1080/03079457.2013.845292 CrossRefGoogle Scholar
  17. Rauw F, Gardin Y, Palya V, Van Borm S, Gonze M, Lemaire S, van den Berg T, Lambrecht B (2009) Humoral, cell-mediated and mucosal immunity induced by oculo-nasal vaccination of one-day-old SPF and conventional layer chicks with two different live Newcastle disease vaccines. Vaccine 27:3631–3642.  https://doi.org/10.1016/j.vaccine.2009.03.068 CrossRefGoogle Scholar
  18. Susta L, Miller PJ, Afonso CL, Brown CC (2011) Clinicopathological characterization in poultry of three strains of Newcastle disease virus isolated from recent outbreaks. Vet Pathol 48:349–360.  https://doi.org/10.1177/0300985810375806 CrossRefGoogle Scholar
  19. Wambura PN, Kapaga AM, Hyera JMK (2000) Experimental trials with a thermostable Newcastle disease virus (strain I-2) in commercial and village chickens in Tanzania. Prev Vet Med 43:75–83.  https://doi.org/10.1016/S0167-5877(99)00089-6 CrossRefGoogle Scholar
  20. Wambura PN, Meers J, Spradbrow P (2006) Determination of organ tropism of Newcastle disease virus (strain I-2) by virus isolation and reverse transcription-polymerase chain reaction. Vet Res Commun 30:697–706.  https://doi.org/10.1007/s11259-006-3291-7 CrossRefGoogle Scholar
  21. Yan Z, Du Y, Zhao Q, Fan Q, Guo W, Ma R, Wang X, Zhu Z (2011) Mucosal immune responses against live Newcastle disease vaccine in immunosuppressed chickens. Pak Vet J 31:280–286Google Scholar
  22. Zhang X, Zhang X, Yang Q (2007) Effect of compound mucosal immune adjuvant on mucosal and systemic immune responses in chicken orally vaccinated with attenuated Newcastle-disease vaccine. Vaccine 25:3254–3262.  https://doi.org/10.1016/j.vaccine.2007.01.052 CrossRefGoogle Scholar

Copyright information

© Springer-Verlag London Ltd., part of Springer Nature 2018

Authors and Affiliations

  1. 1.Poultry Diseases Research Center, Avian Medicine Section, Department of Clinical Sciences, School of Veterinary MedicineShiraz UniversityShirazIran
  2. 2.Group of Biological Sciences, Faculty of SciencesUniversity of IsfahanIsfahanIran

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