Evaluation of the toxicopathological lesions of Calotropis procera using a chick embryonic model
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Toxicopathological effects of herbs have always been a major concern. There is scant information available about the toxicopathological effects of Calotropis procera (C. procera) in the fetus. Since the chick embryo is a suitable preclinical model to evaluate the toxicopathological effects of chemicals, the objective of this study is to evaluate the lesions of various dosages of C. procera using a chick embryonic model. Fertile chicken eggs were divided into three equal treatment groups; phosphate buffered saline-injected group and C. procera-injected groups whose individuals were treated with C. procera extract at dosages of 50 or 100 mg/kg egg-weight. Embryos were re-incubated post-treatment and allowed to develop until day 18, after which they were examined for macroscopic and microscopic lesions. Although the embryos which were treated with 50 mg/kg egg-weight of C. procera extract macroscopically were normal as well as the controls, those treated with 100 mg/kg egg-weight were stunted and under developed. Microscopic evaluations showed that in embryos treated with 100 mg/kg egg-weight of C. procera, the brain was congested, and severe dilation of central veins and sinusoids as well as hepatocellular degeneration was occurred in liver. Moreover, the kidney and lung were under-developed, but the structure of the heart was normal. Based on our findings, C. procera at dosage of 100 mg/kg is toxic to the chick embryo or/maybe to human fetus during growing period. Further studies are needed to clarify the toxic effects of this plant on the development of fetus.
KeywordsCalotropis procera Chick Embryo Fetus Pathology
The authors wish to thank Mr. S. Hasanzadeh for his kind cooperation in tissue slide preparation.
This study was financially supported by grant no. 94-01-45-10742 from Vice-Chancellery of Research of Shiraz University of Medical Sciences, Shiraz, Iran.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.
- Akinloye A, Abatan M, Alaka O, Oke B (2002) Histomorphometric and histopathological studies on the effect of Calotropis procera (giant milkweed) on the male reproductive organs of wistar rats. Afr J Biomed Res 5Google Scholar
- Al-Snafi A (2015) The constituents and pharmacological properties of Calotropis procera-an overview. Int J Pharm Rev Res 5:259–275Google Scholar
- Balouiri M, Bouhdid S, Harki E, Sadiki M, Ouedrhiri W, Ibnsouda SK (2015) Antifungal activity of Bacillus spp. isolated from Calotropis procera AIT. Rhizosphere against Candida albicans. Asian J Pharm Clin Res 8:213–217Google Scholar
- Derakhshanfar A, Tavakkoli H (2016) Evaluation of the embryonic pathological lesions and efficacy of amantadine against H9N2 influenza virus using chicken embryo model. J Kerman Univ Med Sci 23:554–571Google Scholar
- Hamburger V (1942) A manual of experimental embryology. University of Chicago Press, ChicagoGoogle Scholar
- Jain P, Bansal D, Bhasin P (2015) Antimicrobial activity and phytochemical screening of five wild plants against Escherichia coli, Bacillus subtilis and Staphylococcus aureus. J Pharm Res 1260–1262Google Scholar
- Kumar S, Gupta A, Pandey AK (2013) Calotropis procera root extract has the capability to combat free radical mediated damage. ISRN Pharmacol 2013Google Scholar
- Kumar VL, Chaudhary P, Ramos MV, Mohan M, Matos MP (2011) Protective effect of proteins derived from the latex of Calotropis procera against inflammatory hyperalgesia in monoarthritic rats. Phytother Res 25:1336–1341Google Scholar
- Mosallanejad SS, Tavakkoli H, Derakhshanfar A, Salandari S (2014) An experimental study of the systemic alteration of nitroimidazoles in the middle stage of embryonic development. Int J Adv Biol Biomed Res 2:1468–1474Google Scholar
- Nazem MN, Amanollahi R, Tavakoli H, Mansouri F (2015) Effect of in ovo injected methionine on feather follicle formation and its growth in the chicken. Embryo Anat Sci J 12:83–88Google Scholar
- Punia G (2013) A review on varieties of arka-calotropis procera (aiton) dryand and calotropis gigantea (L.) dryand. Glob J Res Med Plants Indigenous Med 2:392Google Scholar
- Rahmatullah M et al (2010) Effect of Cuscuta reflexa stem and Calotropis procera leaf extracts on glucose tolerance in glucose-induced hyperglycemic rats and mice. Afr J Tradit Complement Altern Med 7:109–112Google Scholar
- Samia M, Adam S, Shigidi M, Hapke H (1998) Studies on laticiferous plants: toxic effects in goats of Calotropis procera latex given by different routes of administration DTW. Dtsch Tierarztl Wochenschr 105:425–427Google Scholar
- Singh R, Mittal P, Dhiman R (2005) Laboratory study on larvicidal properties of leaf extract of Calotropis procera (family-Asclepiadaceae) against mosquito larvae. J Commun Dis 37:109Google Scholar
- Singroha R, Srivastava S, Chhikara P (2012) Effect of gentamicin on kidney in developing chicks. Eur J Anat 16:119–126Google Scholar
- Tavakkoli H, Derakhshanfar A, Noori Gooshki S (2013) A short preliminary experimental study on teratogenic effect of methenamine in embryonic model. Int J Adv Biol Biomed Res 1:1523–1528Google Scholar
- Tavakkoli H, Derakhshanfar A, Noori Gooshki S (2014a) Toxicopathological lesions of fosfomycin in embryonic model. Eur J Exp Biol 4:63–71Google Scholar
- Tavakkoli H, Derakhshanfar A, Salandari S (2014b) Effect of dietary Pistacia atlantica oil on performance and pathological conditions in chukar partridge. J Livest Sci Technol 2:19–25Google Scholar
- Traore A, Lompo M, Some N, Sana B, Guissou I (2011) Vasodilator effect of Zanthoxylum zanthoxyloïdes, Calotropis procera and FACA, a mixture of these two plants. Int J Biol Chem Sci 5:1351–1357Google Scholar