Advertisement

Comparative hypolipidaemic effects of crude seed powder, aqueous and methanolic seed extracts of Buchholzia coriacea in carbon tetrachloride-induced toxicity in albino rats (Rattus norvegicus)

  • Ugwu Godwin Chigozie
  • Eyo Joseph Effiong
  • Okanya Chinagorom Laureta
  • Egbuji Jude Ifeanyi
  • Okwor Jude Ifeanyi
  • Nnamonu Emmanuel Ikechukwu
Original Article

Abstract

Comparative hypolipidaemic effects of aqueous seed extracts, methanolic seed extracts and crude seed powder of Buchholzia coriacea in rats intoxicated with carbon tetrachloride were evaluated for 56 days. A total of male albino rats (150–200 g) comprising of 12 normal and 132 CCl4-induced toxic rats were divided into 4 major groups: control groups (normal, positive and negative controls) and 3 treatment groups (B. coriacea aqueous extract (BCAE) group, B. coriacea methanolic extract (BCME) group and B. coriacea crude powder (BCCP) group) of 36 albino rats. The major group was split into three sub-groups consisting of three replicates of four rats each. The normal, negative and positive control groups were given 1 ml/kg distilled water, 150 mg/kg of CCl4 and 200 mg/kg of silymarin respectively, while each treatment group was administered 200, 400 and 800 mg/kg respectively. Serum samples were collected from selected rats in each replicate of both treatment and control groups after 7 days of intoxication and at 2 weeks interval for various lipid profile parameters using standard methods. A time-independent significant decrease (p < 0.05) was observed in total cholesterol; LDL-cholesterol, triglycerides, VLDL-cholesterol and significant increase (P < 0.05) in HDL-cholesterol were observed in hepatotoxic rats treated with the BCCP, BCAE and BCME in comparison with the control groups. Conclusively, post treatment with aqueous seed extracts, methanolic seed extracts and crude seed powder of B. coriacea significantly attenuated hyperlipidaemic activities. Therefore, it can be suggested that B. coriacea extracts could have a protective effects against cardiovascular diseases.

Keywords

Buchholzia coriacea Methanolic extracts Aqueous extracts Crude seed powder Lipid profile Albino rats 

Notes

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

Handling of experimental animals used in this research was in accordance with that recommended by the Committee and the International Guidelines for Handling of Laboratory Animals (Derrell 1996).

Informed consent

Informed consent was obtained from each participants included in the study.

References

  1. Aashish P, Tarun S, Pallavi B (2013) Ameliorative effect of leaves of Carica papaya in ethanol and antitubercular drug induced hepatotoxicity. Bri J Pharm Res 3(4):648–661CrossRefGoogle Scholar
  2. Ahmad M, Saeed MM, Alam H, Ashgar Z (1992) Biological studies of indigenous medicinal plants ii: effort of aplotaxix lappa dcne on various parameters of liver metabolism in rabbits. J Islam Acad Sc 5:61–66Google Scholar
  3. Brown SB, Goldstein JL (1992) Drugs used in the treatment of hyperlipoproteinaemias. In: Galman AG, Rall TW, Nies AS, Taylor P (eds) Goodman and Gilman’s pharmacological basis of therapeutics, vol 1, 8th edn. McGraw-Hill Incorporation, New YorkGoogle Scholar
  4. Derrell C (1996) Guide for the care and use of laboratory animals. Institute of Laboratory Animal Resources. National Academy Press, Washington DCGoogle Scholar
  5. Duru M, Amadi B, Ugbogu A, Eze (2014) Effect of “udu”, an antimalarial herbal preparation on visceral organ weight and blood lipid profiles in wistar rats. J Physics: conference series 8:1–7Google Scholar
  6. Edem DO (2002) Palm oil: Biochemical, physiological, nutritional, haematological and toxicological aspects: a review Plant Food Human Nutr 57:319–341Google Scholar
  7. Enechi OC, Manyawo LN, Ugwu OPC (2013) Effect of ethanol seed extract of Buchholzia coriacea (wonderful kola) on the lipid profile of albino rats. Afr J Biotechnol 12(32):5075–5079CrossRefGoogle Scholar
  8. Ezeja MI, Ezeigbo II, Madubuike KG (2011) Analgesic activity of the methanolic seed extract of Buchholzia coriacea. Res J Pharm Biol Chem Sci 2:187–193Google Scholar
  9. Ezekwesili CN, Obidoa O, Nwodo OFC (2008) Effects of ethanol extract of Acalypha torta leaves on the ipid profile and serum electrolytes of rabbit. Nig J Biochem Mole Biol 23(1):15–19Google Scholar
  10. Fred-Jaiyesimi AA, Egbebunmi O, Anthony O (2011) Larvicidal effect of he pet ether, chloroform fractions and methanol extract of Buchholzia coriacea engle seed. Int J Pharm Sci Res 2(7):1736–1739Google Scholar
  11. Friedwald WT, Levy RI, Fredrickson DS (1972) Estimation of the concentration of low density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem 18(6):499–502Google Scholar
  12. Ghasi S, Nwobodo E, Ofili JO (2002) Hypocholesterolemic effects of crude extract of leaf of Moringa oleifera Linn. in high-fat diet wistar rats. J Ethnopharmacol 69:21–25CrossRefGoogle Scholar
  13. Grew RH (2006) Lipid metabolism II: pathways of metabolism of special lipids. In: Delvlin TM (ed) Test book of biochemistry with clinical correlations, 6th edn. Wiley Liss, New JerseyGoogle Scholar
  14. Hoff J (2000) Methods of blood collection in the mouse. Lab Anim 29(10):50–51Google Scholar
  15. Ibrahim TA, Fagbohun ED (2014) Phytochemical and nutritive qualities of dried seeds of Buchholzia coriacea: research and reviews. J Food Dairy Technol 2(2):1–7Google Scholar
  16. Ibrahim TA, Fagbonun ED (2013) Antibacterial and antifungal activity of ethanolic and methanolic extract of dried seeds of Buchholzia coriacea. Greener J Agric Sci 3(6):458–463Google Scholar
  17. Imafidon KE (2010) Tissue lipid profile of rats administered aqueous extract of Hibiscus rosasinensi. Linn. J Basic Appl Sci 6(1):1–3Google Scholar
  18. Lipid Research Clinics Programme (1984) The lipid research clinics coronary primary prevention trial results. 1: Reduction in the incidence of heart disease. JAMA 251:351–364CrossRefGoogle Scholar
  19. Manikandaselvi S, Ezhilarasi S, Vaithehi R (2012) Modulation of lysosomal enzymes activity by Carica papaya Linn. and Solanum torvum Linn. in carbon tetrachloride vapour induced liver damage in rats. J Chem Pharm Res 4(2):1235–1238Google Scholar
  20. Mbata TI, Duru CM, Onwumelu HA (2009) Antibacterial activity of crude seed extract of Buchholzia coriacea E. on some pathogenic bacterials. J Dev Biol Tissue Eng 1(1):1–5Google Scholar
  21. National Cholesterol Education Program (NCEP) Expert Panel (2001) Third report of the National Cholesterol Education Program (NCEP) expert panel on detection, evaluation, and treatment of high blood cholesterol in adults (ATP 111). NIH Publication. Bethesda: National Heart, Lung, and Blood InstituteGoogle Scholar
  22. Nwachukwu MI, Duru MKC, Amadi BA, Nwachukwu IO (2014) Comparative evaluation of phytoconstituents, antibacterial activities and proximate contents of fresh, oven dried uncooked and cooked samples of Buchholzia coriacea seed and their effects on hepatocellular integrity. Int J Pharm Sci Invention 3(6):41–49Google Scholar
  23. Okere OS, Iliemene UD, Tese T, Mubarak L, Olowoniyi OD (2014) Proximate analysis, phytochemical screening and antitrypanocidal potentials of Buchholzia coriacea in Trypanosoma brucei brucei-infected mice. J Pharm Biol Sci 9(4):69–77Google Scholar
  24. Okoli BJ, Okere OS, Adeyemo SO (2010) The antiplasmodic activity of Buchholzia coriacea. J Med Appl Biosci 2:21–29Google Scholar
  25. Olaiya CO, Omolekan TO (2013) Antihypercholesterolemic activity of ethanolic extract of Buchholzia coriacea in rats. Afr Health Sci 13(4):1084–1090PubMedPubMedCentralGoogle Scholar
  26. Olantunji LA, Adebayo JO, Oguntoye OB, Olatunde NO, Olantunji VA, Soladoye AO (2005) Effects of aqueous extracts of petals of red and green Hibiscus sabdariffa on plasma lipid and hematological variables in rats. Pharm Biol 43(5):471–474CrossRefGoogle Scholar
  27. Olusenyi EO, Onyeoziri NF (2009) Preliminary studies on the antimicrobial properties of Buchholzia coriacea (wonderful kola). Afr J Biotechnol 8(3):472–474Google Scholar
  28. Sharma V (2011) Flowers of woodfordia fruticosa exhibit in vitro cytotoxic effect on HEP-2 and SK-N-MC cancer cells. Biotechnol Bioinformatics Bioeng 1(2):229–233Google Scholar
  29. Siddiqui AA, Wani SM, Rajesh R, Alargarsamy A (2006) Phytochemical and Pharmacological Investigation of Hibiscus rosasinensis Linn. Indian J Pharm Sci 68(5):588–593CrossRefGoogle Scholar
  30. Sood R (2006) Medical laboratory technology. Jaypee Brothers medical Publishers Limited, New DelhiCrossRefGoogle Scholar
  31. Ugwu OPC, Nwodo OFC, Joshua PE, Odo CE, Ossai EC (2013) Effect of ethanol leaf extract of Moringa oleifera on lipid profile of mice. Res J Pharm Biol Chem Sci 4(1):1324–1332Google Scholar
  32. Ugwu GC, Eyo JE, Egbuji JI, Ezechukwu C, Ibemenuga KN (2017) Comparative phytochemical and antioxidant profile of crude seed poder, aqueous and methanolic seed extracts of Buchholzia coriacea. Int J Phytomed 9(4):558–565CrossRefGoogle Scholar
  33. Walker S, McMahon D (2008) Biochemistry demystified a self-teaching guide. McGraw Hill, USAGoogle Scholar

Copyright information

© Springer-Verlag London Ltd., part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Zoology and Environmental BiologyUniversity of NigeriaNsukkaNigeria

Personalised recommendations