Comparative Clinical Pathology

, Volume 28, Issue 1, pp 33–40 | Cite as

Prognostic relevance of CD69 expression in B cell chronic lymphocytic leukemia

  • Salah ArefEmail author
  • Nadia El Menshawy
  • Mohamed Sabry El-Ghonemy
  • Ghada El-Said
  • Mohamed Ali El-Baiomy
Original Article


This study aimed to determine the prognostic impact of CD69 expression in B cell chronic lymphocytic leukemia (CLL). The study included 153 B cell chronic lymphocytic leukemia (B-CLL) recruited to Mansoura Oncology Center. Cellular CD69 expression was determined by multicolor flow cytometry in parallel with PCR amplification and sequencing analysis to mutational status of immunoglobulin variable heavy chain (IgVH). The number of CLL patients positively expressed CD69 was 47 out of 153 (30.7%). There is significant association between CD69-positive expression and unmutated IgVH and between CD69 negative expressions with mutated IgVH gene (P < 0.007). The patients positively expressed CD69 were younger in age and had higher leukocyte count, advanced RIA stages III and IV, and high β2 microglobulin as compared to negative ones. CLL patients group positively expressed CD69 had short overall survival and progression disease-free survival (PDFS) as compared to negative ones. Cox proportional hazard regression model showed that positive CD69 expression is an independent prognostic biomarker for progression-free survival (HR for CD69 vs IgVH was 1.7 vs 1.6, p = 0.04 vs 0.02) and OS (HR 1.8 vs 1.9, p = 0.03 vs 0.01). In conclusion, positive CD69 expression could predict adverse outcome; it could be included in scoring system of CLL as simple, easy applicable to stratify early progressive patients and enabling timely therapeutic decisions to improve outcome. Also, it could be a target for therapy for B cell CLL.


CLL CD69 Prognosis 


Compliance with ethical standards

This study did not include animals.

Informed consent was taken from all subjects participate in this study.

Conflict of interest

The authors declare that there is no conflict of interest.


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Copyright information

© Springer-Verlag London Ltd., part of Springer Nature 2018

Authors and Affiliations

  1. 1.Hematology Unit, Clinical pathology DepartmentMansoura University Oncology Center, Mansoura Faculty of MedicineMansouraEgypt
  2. 2.Internal Medicine DepartmentMansoura University Oncology Center, Mansoura Faculty of MedicineMansouraEgypt
  3. 3.Medical Oncology unitMansoura University Oncology Center, Mansoura Faculty of MedicineMansouraEgypt

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