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Initial microbial status modulates mycorrhizal inoculation effect on rhizosphere microbial communities

  • Frédérique ChangeyEmail author
  • Hacène Meglouli
  • Joël Fontaine
  • Maryline Magnin-Robert
  • Benoit Tisserant
  • Thomas Z. Lerch
  • Anissa Lounès-Hadj Sahraoui
Original Article


Arbuscular mycorrhizal fungi (AMF) play a central role in rhizosphere functioning as they interact with both plants and soil microbial communities. The conditions in which AMF modify plant physiology and microbial communities in the rhizosphere are still poorly understood. In the present study, four different plant species, (clover, alfalfa, ryegrass, tall fescue) were cultivated in either sterilized (γ ray) or non-sterilized soil and either inoculated with a commercial AMF (Glomus LPA Val 1.) or not. After 20 weeks of cultivation, the mycorrhizal rate and shoot and root biomasses were measured. The abundance and composition of bacteria, archaea, and fungi were analyzed, respectively, by quantitative PCR (qPCR) and fingerprinting techniques. Whilst sterilization did not change the AMF capacity to modify plant biomass, significant changes in microbial communities were observed, depending on the taxon and the associated plant. AMF inoculation decreases both bacterial and archaeal abundance and diversity, with a greatest extent in sterilized samples. These results also show that AMF exert different selections on soil microbial communities according to the plant species they are associated with. This study suggests that the initial abundance and diversity of rhizosphere microbial communities should be considered when introducing AMF to cultures.


Rhizosphere Sterilization Soil microbial communities Arbuscular mycorrhizal fungi Archaea 


Funding information

This work has been carried out in the Halluin3R project, which is financed by the European Union (FEDER), the French Region of Hauts-de-France, and the French Environment and Energy Management Agency (ADEME) and in the framework of the Alibiotech project which is financed by the European Union, the French State, and the French Region of Hauts-de-France.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

572_2019_914_MOESM1_ESM.doc (266 kb)
ESM 1 (DOC 265 kb)


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© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Unité de Chimie Environnementale et Interactions sur le Vivant) (UCEIV), EA 4492, SFR Condorcet FR CNRS 3417Université du Littoral Côte d’OpaleCalaisFrance
  2. 2.Paris Institute of Ecology and Environnemental Sciences (IEES-Paris), UMR 7518 (CNRS- SU-INRA-UPEC- Paris Diderot-IRD)Université Paris-Est CréteilCréteil CedexFrance

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