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Mycorrhiza

, Volume 28, Issue 7, pp 635–650 | Cite as

Arbuscular mycorrhizal inoculum sources influence bacterial, archaeal, and fungal communities’ structures of historically dioxin/furan-contaminated soil but not the pollutant dissipation rate

  • H. Meglouli
  • A. Lounès-Hadj Sahraoui
  • M. Magnin-Robert
  • B. Tisserant
  • M. Hijri
  • J. Fontaine
Original Article
  • 191 Downloads

Abstract

Little is known about the influence of arbuscular mycorrhizal fungi (AMF) inoculum sources on phytoremediation efficiency. Therefore, the aim of this study was to compare the effects of two mycorrhizal inocula (indigenous and commercial inocula) in association with alfalfa and tall fescue on the plant growth, the bacterial, fungal, and archaeal communities, and on the removal of dioxin/furan (PCDD/F) from a historically polluted soil after 24 weeks of culture in microcosms. Our results showed that both mycorrhizal indigenous and commercial inocula were able to colonize plant roots, and the growth response depends on the AMF inoculum. Nevertheless, the improvement of root dry weight in inoculated alfalfa with indigenous inoculum and in inoculated tall fescue with commercial inoculum was clearly correlated with the highest mycorrhizal colonization of the roots in both plant species. The highest shoot dry weight was obtained in inoculated alfalfa and tall fescue with the commercial inoculum. AMF inoculation differently affected the number of bacterial and archaeal OTUs and bacterial diversity, with elevated bacterial and archaeal OTUs and bacterial diversity observed with indigenous inoculum. Mycorrhizal inoculation increases the abundance of bacterial OTUs (in particular with indigenous inoculum) and microbial richness but it does not improve PCDD/F dissipation. Vegetation had no effect on the abundance of microbial OTUs nor on richness but stimulated specific communities (Planctomycetia and Gammaproteobacteria) likely to be involved in the dissipation of PCDD/F. The reduction of toxic equivalency PCDD/F concentration also could be explained by the stimulation of soil microbial activities estimated with dehydrogenase and fluorescein diacetate hydrolase.

Keywords

Phytoremediation Mycorrhizae Inoculum sources Microbial community Dioxins/furans 

Notes

Acknowledgments

This work has been carried out in the Halluin3R project which is financed by the European Union (FEDER), the French Region of Hauts-de-France and the French Environment and Energy Management Agency (ADEME) and in the framework of the Alibiotech project which is financed by European Union, French State and the French Region of Hauts-de-France. The authors are grateful to the soil analysis laboratory of INRA (Arras, France) for help in PCDD/F analysis. Data presented in this paper were analyzed using the CALCULCO computing platform, supported by SCOSI/ULCO (Service commun du Système d’Information de l’Université du Littoral Côte d’Opale).

Supplementary material

572_2018_852_MOESM1_ESM.docx (368 kb)
ESM 1 (DOCX 367 kb)
572_2018_852_MOESM2_ESM.xlsx (3.5 mb)
ESM 2 (XLSX 3626 kb)

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Authors and Affiliations

  1. 1.Unité de Chimie Environnementale et Interactions sur le Vivant (UCEIV)Université du Littoral Côte d’OpaleCalaisFrance
  2. 2.Institut de Recherche en Biologie Végétale, Département de Sciences BiologiquesUniversité de MontréalMontréalCanada

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