Abiotic contexts consistently influence mycorrhiza functioning independently of the composition of synthetic arbuscular mycorrhizal fungal communities
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The relationship between mycorrhiza functioning and composition of arbuscular mycorrhizal (AM) fungal communities is an important but experimentally still rather little explored topic. The main aim of this study was thus to link magnitude of plant benefits from AM symbiosis in different abiotic contexts with quantitative changes in AM fungal community composition. A synthetic AM fungal community inoculated to the model host plant Medicago truncatula was exposed to four different abiotic contexts, namely drought, elevated phosphorus availability, and shading, as compared to standard cultivation conditions, for two cultivation cycles. Growth and phosphorus uptake of the host plants was evaluated along with the quantitative composition of the synthetic AM fungal community. Abiotic context consistently influenced mycorrhiza functioning in terms of plant benefits, and the effects were clearly linked to the P requirement of non-inoculated control plants. In contrast, the abiotic context only had a small and transient effect on the quantitative AM fungal community composition. Our findings suggest no relationship between the degree of mutualism in AM symbiosis and the relative abundances of AM fungal species in communities in our simplified model system. The observed progressive dominance of one AM fungal species indicates an important role of different growth rates of AM fungal species for the establishment of AM fungal communities in simplified systems such as agroecosystems.
KeywordsPre-conditioning Mycorrhizal functioning Community qPCR Phosphorus Medicago truncatula
The study was supported by the Czech Science Foundation (project GA15-05466S) and by long-term research development programs RVO 67985939 and RVO 61388971.
MJ, JJ, DP, and MV designed the study. AV and DP performed the research. AV and PŠ analyzed data. PŠ contributed new models. AV wrote the paper with a substantial contribution from MJ, JJ, DP, and MV.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
- Gehring CA, Mueller RC, Haskins KE, Rubiw TK, Whitham TG (2014) Convergence in mycorrhizal fungal communities due to drought, plant competition, parasitism, and susceptibility to herbivory: consequences for fungi and host plants. Front Microbiol 5:1–9. https://doi.org/10.3389/fmicb.2014.00306 CrossRefGoogle Scholar
- Jackman, S. (2017) pscl: classes and methods for R developed in the Political Science Computational Laboratory. United States Studies Centre, University of Sydney. Sydney, New South Wales, Australia. R package version 15.2. https://github.com/atahk/pscl/. Accessed 21 April 2017
- Jansa J, Finlay R, Wallander H, Smith FA, Smith SE (2011) Role of mycorrhizal symbioses in phosphorus cycling. In: Bünemann E, Oberson A, Frossard E (eds) Phosphorus in action: biological processes in soil phosphorus cycling, Soil Biol, vol 26. Springer, Heidelberg, pp 137–168. https://doi.org/10.1007/978-3-642-15271-9_6 CrossRefGoogle Scholar
- Johnson D, Vandenkoornhuyse PJ, Leake JR, Gilbert L, Booth RE, Grime JP, Young JPW, Read DJ (2004) Plant communities affect arbuscular mycorrhizal fungal diversity and community composition in grassland microcosms. New Phytol 161:503–515. https://doi.org/10.1046/j.1469-8137.2003.00938.x CrossRefGoogle Scholar
- Konvalinková T, Püschel D, Janoušková M, Gryndler M, Jansa J (2015) Duration and intensity of shade differentially affects mycorrhizal growth- and phosphorus uptake responses of Medicago truncatula. Front Plant Sci 6(65). https://doi.org/10.3389/fpls.2015.00065
- Krak K, Janoušková M, Caklová P, Vosátka M, Štorchová H (2012) Intraradical dynamics of two coexisting isolates of the arbuscular mycorrhizal fungus Glomus intraradices sensu lato as estimated by real-time PCR of mitochondrial DNA. Appl Environ Microbiol 78:3630–3637. https://doi.org/10.1128/AEM.00035-12 CrossRefGoogle Scholar
- Ohno T, Zibilske LM (1991) Determination of low concentrations of phosphorus insoil extracts using malachite green. Soil Sci Soc Am J 55:892–895. https://doi.org/10.2136/sssaj1991.03615995005500030046x CrossRefGoogle Scholar
- Püschel D, Janoušková M, Voříšková A, Gryndlerová H, Vosátka M, Jansa J (2017) Arbuscular mycorrhiza stimulates biological nitrogen fixation in two Medicago spp. through improved phosphorus acquisition. Front Plant Sci 8(380). https://doi.org/10.3389/fpls.2017.00390
- Řezáčová V, Zemková L, Beskid O, Püschel D, Konvalinková T, Hujslová M, Slavíková R, Jansa J (2018) Little cross-feeding of the mycorrhizal networks shared between C3-Panicum bisulcatum and C4-Panicum maximum under different temperature regimes. Front Plant Sci 9:1–16. https://doi.org/10.3389/fpls.2018.00449 CrossRefGoogle Scholar
- Smith SE, Read DJ (2008) Mycorrhizal symbiosis, 3rd edn. Academic Press, LondonGoogle Scholar
- Symanczik S, Courty PE, Boller T, Wiemken A, Al-Yahya’ei MN (2015) Impact of water regimes on an experimental community of four desert arbuscular mycorrhizal fungal (AMF) species, as affected by the introduction of a non-native AMF species. Mycorrhiza 25:639–647. https://doi.org/10.1007/s00572-015-0638-3 CrossRefGoogle Scholar
- Voříšková A, Jansa J, Püschel D, Krüger M, Cajthaml T, Vosátka M, Janoušková M (2017) Real-time PCR quantification of arbuscular mycorrhizal fungi: does the use of nuclear or mitochondrial markers make a difference? Mycorrhiza 27:577–585. https://doi.org/10.1007/s00572-017-0777-9 CrossRefGoogle Scholar