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Meat and fish freshness evaluation by functionalized cantilever-based biosensors


Meat spoilage is a result of an increase in the number of microorganisms and increased levels of biogenic amines (e.g. cadaverine). Destruction of non-spoiled meat products results in substantial waste of resources each year. Currently, the expiration date of meat-products is determined by either subjective sensory and/or expensive and time-consuming microbiological analysis. Cadaverine levels have been demonstrated to be related to the product freshness; however current sensing methods require bulky and expensive chromatography techniques. In this work, the cadaverine binding to a functionalization layer of cyclam (1,4,8,11-tetraazacyclotetradecane) is demonstrated by a systematic study of cyclam/solvent solution influence on morphology and binding. The degradation of the functionalization layer due to storage conditions has also been investigated, and the optimum solvent for the functionalization solution is found to be ethoxyethanol. Functionalized surfaces and cantilevers have been exposed to different types of meat (beef, fish, chicken or pork) and the cadaverine binding has been demonstrated, either by morphology changes (surfaces) or by changes on cantilever resonance frequency due to mass increase (cantilever). The results show a higher cadaverine emission rate for fish, followed by chicken, beef and finally pork.

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  1. Bellarby J, Foereid B, Hastings A, Smith P (2008) Cool farming: climate impacts of agriculture and migration potential. Report produced by the University of Aberdeen for Greenpeace

  2. Food Safety Authority of Ireland (2014) Validation of product shelf-life. Food Safety Authority of Ireland, Dublin

  3. Halasz A, Barath A, Simon-Sarkadi L, Holzapfel W (1994) Biogenic amines and their production by microorganisms in food. J Food Sci Technol 5:42

  4. Hermansen J, Olesen J (2009) Landbrugets og fødevareproduktionens klimapåvirkning. Vand Og Jord 16:137–139

  5. Kowalski Z, Krupa-Zuczek K (2007) A model of the meat waste management. Pol J Chem Technol 9(4):91

  6. Lehotay SJ, Hajslova J (2002) Application of gas chromatography in food analysis. TrAC Trends Anal Chem 21:686–697

  7. Leuschner RG, Kurthara M, Hammes WP (1999) Formation of biogenic amines by proteolytic enterococci during cheese ripening. J Sci Food Agric 79:1141

  8. Nishikawa H et al (2012) Simple detection method of biogenic amines in decomposed fish by intramolecular excimer fluorescence. Food Nutr Sci 3:1020–1026

  9. Rauscher-Gabernig E et al (2012) Dietary exposure assessment of putrescine and cadaverine and derivation of tolerable levels in selected foods consumed in Austria. Eur Food Res Technol 235:209–220

  10. Vinci G, Antonelli ML (2002) Biogenic amines: quality index of freshness in red and white meat. Food Control 13:519

  11. Wang Y et al (2016) Functionalizing micro-cantilevers for meat degradation measurements. In: Symposium on design, test, integration and packaging of MEMS/MOEMS (DTIP)

  12. Wang Y et al (2017) Micro-cantilevers for optical sensing of biogenic amines. Microsyst Technol 24:363–369

  13. Zhao X et al (2009) Determination of five biogenic amines in source water by ion chromatography. Chin J Chromatogr 27(4):505

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Correspondence to Roana de Oliveira Hansen.

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Costa, C.A.B., Grazhdan, D., Fiutowski, J. et al. Meat and fish freshness evaluation by functionalized cantilever-based biosensors. Microsyst Technol 26, 867–871 (2020). https://doi.org/10.1007/s00542-019-04598-7

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