Microfluidic platform for dielectrophoretic separation of bio-particles using serpentine microelectrodes

  • Paridhi PuriEmail author
  • Vijay Kumar
  • Sachin U. Belgamwar
  • M. Ananthasubramanian
  • N. N Sharma
Technical Paper


Recently, there is a great concern in developing integrated microfluidic platforms for biological analysis systems that can perform general biochemistry and biological analysis. Dielectrophoresis, the movement of particles in a non-uniform electric field is widely used to separate viable and non viable cells with good efficiency. The present study aims to develop a universal bio-particle separator in a C-serpentine geometry which could separate particles by varying the operating conditions. We present the design, fabrication and testing for continuous separation structure based on dielectrophoretic forces. The C-Serpentine geometry is composed of consecutive C shaped curves to generate a gradient required for the dielectrophoretic effect. Known mixture of viable and non viable cells of Saccharomyces Cerevisiae was selectively trapped using negative dielectrophoretic force generated by microelectrodes. Through measurement of cell count, a trapping efficiency of 86% was obtained for mixed cell suspension and 93–98% trapping efficiency was obtained in case of trapping live and dead yeast cells individually. Also the results show that cell viability was not affected by the separation procedure as percentage of viable cells was calculated to be 96%.



The authors would like to acknowledge IISc, Bangalore, for the use of fabrication facility and Department of Biology, BITS Pilani for providing support and guidance in cell culturing is also acknowledged.


  1. Chen YT, Lee D (2007) A bonding technique using hydrophilic SU-8. J Micromech Microeng 17(10):1978CrossRefGoogle Scholar
  2. Church C, Zhu J, Wang G, Tzeng TRJ, Xuan X (2009) Electrokinetic focusing and filtration of cells in a serpentine microchannel. Biomicrofluidics 3(4):044109–044118CrossRefGoogle Scholar
  3. Church C, Zhu J, Niteo J, Keten G, Ibarra E, Xuan X (2010) Continuous particle separation in a serpentine microchannel via negative and positive dielectrophoretic focusing. J Micromech Microeng 20(6):065011–065016CrossRefGoogle Scholar
  4. Church C, Zhu J, Xuan X (2011) Negative dielectrophoresis-based particle separation by size in a serpentine microchannel. Electrophoresis 32(5):527–531CrossRefGoogle Scholar
  5. Devi UV, Puri P, Sharma NN, Ananthasubramanian M (2014) Electrokinetics of cells in dielectrophoretic separation: a biological perspective. BioNanoScience 4(3):276–287CrossRefGoogle Scholar
  6. Gonzalez CF, Remcho VT (2005) Harnessing dielectric forces for separations of cells, fine particles and macromolecules. J Chromatogr A 1079(1–2):59–68CrossRefGoogle Scholar
  7. Gray DS, Tan JL, Voldman J, Chen CS (2004) Dielectrophoretic registration of living cells to a microelectrode array. Biosens Bioelectron 19(12):1765–1774CrossRefGoogle Scholar
  8. Huang Y, Holzel R, Pethig R, Wang XB (1992) Differences in the AC electrodynamics of viable and non-viable yeast cells determined through combined dielectrophoresis and electrorotation studies. Phys Med Biol 37(7):1499–1517CrossRefGoogle Scholar
  9. Hunt TP, Westervelt RM (2006) Dielectrophoresis tweezers for single cell manipulation. Biomed Microdevice 8(3):227–230CrossRefGoogle Scholar
  10. Irimajiri A, Hanai T, Inouye A (1979) A dielectric theory of ‘multi-stratified shell’ model with its application to a lymphoma cell. J Theor Biol 78(2):251–269CrossRefGoogle Scholar
  11. Li M, Li S, Cao W, Li W, Wen W, Alici G (2012) Continuous particle focusing in a waved microchannel using negative Dc dielectrophoresis. J Micromech Microeng 22(9):095001–095008CrossRefGoogle Scholar
  12. Markx GH, Carney L, Littlefair M, Sebastian A, Buckle AM (2009) Recreating the hematon: microfabrication of artificial haematopoietic stem cell microniches in vitro using dielectrophoresis. Biomed Microdevice 11(1):143–150CrossRefGoogle Scholar
  13. McCloskey KE, Chalmers JJ, Zborowski M (2003) Magnetic cell separation: characterization of magnetophoretic mobility. Anal Chem 75(24):6868–6874CrossRefGoogle Scholar
  14. Patel S, Showers D, Vedantam P, Tzeng TR, Qian S (2012) Microfluidic separation of live and dead yeast cells using reservoir-based dielectrophoresis. Biomicrofluidics 6(3):34102CrossRefGoogle Scholar
  15. Puri P, Kumar V, Ananthasubramanian M, Sharma NN (2017) Design, simulation and fabrication of MEMS based dielectrophoretic separator for bio-particles. Microsyst Technol 23(8):3371–3379CrossRefGoogle Scholar
  16. Qian C, Huang H, Chen L, Li X, Ge Z, Chen T, Yang Z, Sun L (2014) Dielectrophoresis for bioparticle manipulation. Int J Mol Sci 15(10):18281–18309CrossRefGoogle Scholar
  17. Ramos A, Morgan H, Green NG, Castellanos A (1998) Ac electrokinetics: a review of forces in microelectrode structures. J Phys D Appl Phys 31(18):2338–2353CrossRefGoogle Scholar
  18. Ryll T, Dutina G, Reyes A, Gunson J, Krummen L, Etcheverry T (2000) Performance of small scale CHO perfusion cultures using an acoustic cell filtration device for cell retention: characterization of separation efficiency and impact of perfusion on product quality. Biotechnol Bioeng 69(4):440–449CrossRefGoogle Scholar
  19. Srivastava SK (2015) Recent Trends in Dielectrophoretic Applications towards Medical Diagnostics. J Biosensors Bioelectr 6(2):1000e136Google Scholar
  20. Tuchin VV (2007) A clear vision for laser diagnostics (review). IEEE J Sel Top Quantum Electron 6(13):1621–1628CrossRefGoogle Scholar
  21. Yu L, Tay FEH, Xu G, Chen B, Avram M, Iliescu C (2006) Adhesive bonding with SU-8 at wafer level for microfluidic devices. J Phys Conf Ser IOP Publ 34(1):776CrossRefGoogle Scholar
  22. Zhang J, Li W, Li M, Alici G, Nguyen NT (2014) Particle inertial focusing and its mechanism in a serpentine microchannel. Microfluid Nanofluid 17(2):305–316CrossRefGoogle Scholar
  23. Zhu J, Tzeng TRJ, Hu G, Xuan X (2009) DC dielectrophoretic focusing of particles in a serpentine microchannel. Microfluid Nanofluid 7(6):751–756CrossRefGoogle Scholar
  24. Zhu J, Canter RC, Keten G, Vedantam P, Tzeng TRJ, Xuan X (2011) Continuous-flow particle and cell separations in a serpentine microchannel via curvature-induced dielectrophoresis. Microfluid Nanofluid 11(6):743–752CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Mechanical EngineeringBirla Institute of Technology and SciencePilaniIndia
  2. 2.Semi Conductor LaboratoryMohaliIndia
  3. 3.Department of BiotechnologyPSG College of TechnologyCoimbatoreIndia
  4. 4.School of Automobiles, Mechanical and MechatronicsManipal UniversityJaipurIndia

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