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Journal of Gastroenterology

, Volume 54, Issue 12, pp 1061–1069 | Cite as

A novel urinary microRNA biomarker panel for detecting gastric cancer

  • Hiroyasu Iwasaki
  • Takaya ShimuraEmail author
  • Tamaki Yamada
  • Yusuke Okuda
  • Makoto Natsume
  • Mika Kitagawa
  • Shin-ichi Horike
  • Hiromi Kataoka
Original Article—Alimentary Tract

Abstract

Background

Gastric cancer (GC) is one of the most common causes of cancer deaths worldwide; however, reliable and non-invasive screening methods for GC are not established. Therefore, we conducted this study to develop a biomarker for GC detection, consisting of urinary microRNAs (miRNAs).

Methods

We matched 306 participants by age and sex [153 pairs consisting of patients with GC and healthy controls (HCs)], then randomly divided them across three groups: (1) the discovery cohort (4 pairs); (2) the training cohort (95 pairs); and (3) the validation cohort (54 pairs).

Results

There were 22 urinary miRNAs with significantly aberrant expressions between the two groups in the discovery cohort. Upon multivariate analysis of the training cohort, urinary expression levels of miR-6807-5p and miR-6856-5p were significantly independent biomarkers for diagnosis of GC, in addition to Helicobacter pylori (H. pylori) status. A diagnostic panel that combined these 2 miRNAs and H. pylori status distinguished between HC and GC samples with an area under the curve (AUC) = 0.736. In the validation cohort, urinary miR-6807-5p and miR-6856-5p showed significantly higher expression levels in the GC group, and the combination biomarker panel of miR-6807-5p, miR-6856-5p, and H. pylori status also showed excellent performance (AUC = 0.885). In addition, this biomarker panel could distinguish between HC and stage I GC patients with an AUC = 0.748. Urinary expression levels of miR-6807-5p and miR-6856-5p significantly decreased to undetectable level after curative resection of GC.

Conclusions

This novel biomarker panel enables early and non-invasive detection of GC.

Keywords

Biomarker Gastric cancer Urinary miRNA miR-6807-5p miR-6856-5p 

Notes

Acknowledgements

We thank Yukimi Hashidume-Itoh for handling of urine sample and Takako Onodera for data management of enrolled patients in this study (Department of Gastroenterology and Metabolism, Nagoya City University Graduate School of Medical Sciences). We also thank Masahide Ebi at Aichi Medical University, Tomonori Yamada at Nagoya Daini Red Cross Hospital and other clinical colleagues who assisted for the sample collection. This study was supported, in part, by Japan Agency for Medical Research and Development under Grant Number JP19lm0203005j0003 (to T. S.) and the Takeda Science Foundation (to T. S.)

Supplementary material

535_2019_1601_MOESM1_ESM.doc (139 kb)
Supplementary file1 (DOC 139 kb)
535_2019_1601_MOESM2_ESM.pdf (823 kb)
Supplementary file2 (PDF 822 kb)
535_2019_1601_MOESM3_ESM.xlsx (122 kb)
Supplementary file3 (XLSX 121 kb)

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Copyright information

© Japanese Society of Gastroenterology 2019

Authors and Affiliations

  1. 1.Department of Gastroenterology and MetabolismNagoya City University Graduate School of Medical SciencesNagoyaJapan
  2. 2.Okazaki Public Health Center, OkazakiOkazakiJapan
  3. 3.Advanced Science Research CenterKanazawa UniversityKanazawaJapan

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