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Plant Reproduction

, Volume 32, Issue 4, pp 371–380 | Cite as

Molecular mapping of a novel male-sterile gene msNJ in soybean [Glycine max (L.) Merr.]

  • Zhixing Nie
  • Tuanjie Zhao
  • Meifeng Liu
  • Jinying Dai
  • Tingting He
  • Duo Lyu
  • Jinming Zhao
  • Shouping Yang
  • Junyi GaiEmail author
Original Article
  • 109 Downloads

Abstract

Nuclear male sterility (NMS) is a potential characteristic in crop recurrent selection and hybrid breeding. Mapping of nuclear male-sterile genes is key to utilizing NMS. Previously, we discovered a spontaneous soybean (Glycine max [L.] Merr.) male-sterile female-fertile mutant NJS-13H, which was conferred by a single recessive gene, designated msNJ. In this study, the msNJ was mapped to Chromosome 10 (LG O), and narrowed down between two SSR (simple sequence repeats) markers, BARCSOYSSR_10_794 and BARCSOYSSR_10_819 using three heterozygote-derived segregating populations, i.e., (NJS-13H × NN1138-2)F2, (NJS-13H × N2899)F2 and (NJS-13H)SPAG (segregating populations in advanced generations). This region spans approximately 1.32 Mb, where 27 genes were annotated according to the soybean reference genome sequence (Wm82.a2.v1). Among them, four genes were recognized as candidate genes for msNJ. Comparing to the physical locations of all the known male-sterile loci, msNJ is demonstrated to be a new male-sterile locus. This result may help the utilization and cloning of the gene.

Keywords

Soybean [Glycine max (L.) Merr.] Nuclear male sterility (NMS) Simple sequence repeats (SSR) Gene mapping 

Notes

Acknowledgements

This work was financially supported by grants from the China National Key R&D program (2016YFD0100304, 2017YFD0102002, 2017YFD0101500), the MOE 111 Project (B08025), the MOE Program for Changjiang Scholars and Innovative Research Team in University (PCSIRT_17R55), the MARA CARS-04 program, the Jiangsu Higher Education PAPD Program, the Fundamental Research Funds for the Central Universities (KYT201801) and the Jiangsu JCIC-MCP.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Soybean Research Institute, National Center for Soybean Improvement, MARA Key Laboratory for Biology and Genetic Improvement of Soybean (General), National Key Laboratory for Crop Genetics and Germplasm EnhancementNanjing Agricultural UniversityNanjingPeople’s Republic of China

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