Effect of heat stress on the quantitative expression patterns of different cytokine genes in Malabari goats
A study was conducted to determine the influence of exposure to hot environment on different cytokine gene expression patterns in Malabari goat. The study was conducted in 12 (10 months to 1 year old) goats of Malabari breed for 45-day duration. The control (n = 6; C) group goats were kept under comfortable condition in the shaded pens while heat stress (n = 6; HS) group goats were kept out in the open hot environment during summer season for the study duration between 10.00 h to 16.00 h. The representative mesenteric lymph node (MLN) samples were collected from the slaughtered goats at the end of study for assessing the different cytokine gene expression and histopathological changes. Compared with C group, the expression patterns of interleukin 18 (IL-18), tumor necrosis factor-α (TNF-α), interferon-β (IFN-β), and IFN-γ downregulated (P < 0.05) in the HS group. The histopathological changes of MLNs showed paucity of lymphocyte distribution in follicular areas as wells as decreased density of lymphocytes in the germinal centers of the HS group (P < 0.05). The findings from this study reflected the compromised immune functions during heat stress in Malabari goats. Further, the study established that IL-18, TNF-α, IFN-β, and IFN-γ genes could serve as reliable immunological markers for quantifying heat stress–mediated immune response alterations in Malabari goats.
KeywordsCytokines Heat stress Goat Immunity Interleukins
The authors sincerely thank both the Directors, ICAR-NIANP for providing the research facilities and the Indian Council of Agricultural Research (ICAR) for providing the grant for this study.
Compliance with ethical standards
This study was conducted with the approval of the Institutional Animal Care and Use Committee (IACUC) (NIANP/IAEC/2/2017).
- Al-Rahman SAHA, Baqey DAA (2016) Effect of the thermal changes on physiological, biochemical and histological traits in pregnant and embryo of New Zealand white rabbits. Int J Adv Biol Res 6(2):313–327Google Scholar
- Kumar D, Yadav B, Choudhury S, Kumari P, Madan AK, Singh SP, Rout PK, Ramchandran N, Yadav S (2017) Evaluation of adaptability to different seasons in goat breeds of semi-arid region in India through differential expression pattern of heat shock protein genes. Biol Rhythm Res 15:1–3Google Scholar
- McDowell RE (1972) Improvement of livestock production in warm climate. WH freeman and co, San Fransisco, USAGoogle Scholar
- Mengesha M, Tsega W (2012) Indigenous sheep production in Ethiopia: a review. Iran J App Anim Sci 2:311–318Google Scholar
- Shilja S, Sejian V, Bagath M, Mech A, David CG, Kurien EK, Varma G, Bhatta R (2016) Adaptive capability as indicated by behavioural and physiological responses, plasma HSP70 level, and PBMC HSP70 mRNA expression in Osmanabadi goats subjected to combined (heat and nutritional) stressors. Int J Biometeorol 60:1311–1323CrossRefGoogle Scholar
- Sophia I, Sejian V, Bagath M, Bhatta R (2016) Impact of heat stress on immune responses of livestock: a review. J Trop Agri Sci 39:459–482Google Scholar
- Vandana GD, Bagath M, Sejian V, Krishnan G, Beena V, Bhatta R (2018) Summer season induced heat stress impact on the expression patterns of different toll-like receptor genes in Malabari goats. Biol Rhythm Res:1–17. https://doi.org/10.1080/09291016.2018.1464638