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Belowground responses of woody plants to nitrogen addition in a phosphorus-rich region of northeast China

  • Jing Guo
  • Yingzhi GaoEmail author
  • David M. Eissenstat
  • Chunguang HeEmail author
  • Lianxi Sheng
Original Article
  • 13 Downloads

Abstract

Key Message

Nitrogen addition leads to large increases in shoot growth but limited increases in root growth and reductions in mycorrhizal colonization of Sorbus pohuashanensis and Acanthopanax sessiliflorus.

Abstract

Soil in the cultivated fields of Changbai Mountain region of China is rich in phosphorus (P) and deficient in nitrogen (N) for most woody plants. However, currently N deposition is increasing and reducing its limitation on plant growth. How N addition shifts carbon investment among shoots, roots and arbuscular mycorrhizal (AM) fungi is not well understood, especially in woody plants growing in the field. We examine the responses of the growth, biomass partitioning and AM colonization of Sorbus pohuashanensis Hedl. and Acanthopanax sessiliflorus Seem. to low and high N fertilization in northeastern China on high-P soil over 3 years. With N addition, both plants increased shoot biomass by 20–45%, and N and P content by 13–30%, while root biomass increased only by 2.1–5.4%. The slower increase in root growth relative to shoot growth resulted in lower root mass fraction. After plant size (ontogeny) was accounted for, root mass fraction still decreased significantly with high N fertilization in both species. Mycorrhizal colonization intensity and AM-colonized root length decreased with an increase in N addition. In this P-rich site, the limited increase in root biomass and large decrease in AM colonization with N addition presumably promoted plant growth and nutrient uptake. Our results imply that the growth of these two species may be improved by increased carbon allocation to shoots, as N addition permitted sufficient nutrient uptake by roots and AM fungi to meet shoot nutrient demand without additional belowground carbon expenditure.

Keywords

Arbuscular mycorrhiza Biomass partitioning Carbon investment Sorbus pohuashanensis Acanthopanax sessiliflorus 

Notes

Acknowledgements

This work was financially supported by the National Key Basic Research Program of China (2016YFC0500703, 2016YFC0500407), the National Natural Science Foundation of China (31670446, 31270444) and Human Resources and Social Security Department of Jilin Province (2016-28). We thank anonymous reviewers and editor for their comments and suggestions.

Author contribution statement

JG, YG and CH designed the experiment and performed the study. CH and LS helped to establish and maintain the experimental plots. JG analyzed the data and drafted the first version of the manuscript. JG, YG and DME helped with manuscript revisions. All authors read and approved the final version of this manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

468_2019_1906_MOESM1_ESM.docx (286 kb)
Table S1 Results of repeated measures ANOVA on plant growth, biomass partitioning, plant nutrient content, root length and mycorrhizal colonization traits, with species and fertilization treatment as independent variable, and years as the repeated measure. Table S2 Plant height, nutrient content, N:P ratio, root length and AM colonization (means±SE, n = 4) of two woody plants Sorbus pohuashanensis (Sopo) and Acanthopanax sessiliflorus (Acse) over three years. Differences among three fertilization treatments for each plant traits were analyzed by years. Different letters represent statistical significance (P < 0.05) among treatments. Treatments are: C, control (no additional N addition); L, low nitrogen fertilization (5 g m-2 yr-1); H, high nitrogen fertilization (15 g m-2 yr-1). Table S3 Linear regression between leaf, stem or root mass fraction (dependent variable, y axis, g g-1) and total biomass (independent variable, x axis, kg) of Sorbus pohuashanensis and Acanthopanax sessiliflorus. Treatments are: C, control (no additional N addition); L, low nitrogen fertilization (5 g m-2 yr-1); H, high nitrogen fertilization (15 g m-2 yr-1). Table S4 Analysis of covariance on biomass partitioning of Sorbus pohuashanensis and Acanthopanax sessiliflorus, with leaf, stem or root mass fraction as independent variable individually, control (no additional nitrogen addition) and low or high nitrogen fertilization treatment (NF) as fixed factor, and total biomass (TB) as covariate. Treatments are: C, control (no additional N addition); L, low nitrogen fertilization (5 g m-2 yr-1); H, high nitrogen fertilization (15 g m-2 yr-1). Table S5 Results of repeated measures ANOVA on the traits of absorptive root and mycorrhizal colonization for two woody seedlings, with species and fertilization treatment as independent variable, and years as the repeated measure. All these parameters were measured based on both unfertilized and fertilized plots in 2016 and 2017. Table S6 Traits of absorptive roots and of mycorrhizal colonization (means±SE, n = 8) for two woody seedlings. All parameters are based on both unfertilized and fertilized plots in 2016 and 2017. Table S7 Plant type, soil fertility, N addition amount, and shift in plant P demand, root amount, root fraction and AM colonization after N addition in previous studies. Fig. S1 Location of the study area in Jilin Longwan National Nature Reserve, Jinchuan town, northeastern China. Fig. S2 Pictures of Sorbus pohuashanensis and Acanthopanax sessiliflorus (DOCX 286 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Key Laboratory of Vegetation Ecology, Institute of Grassland ScienceNortheast Normal UniversityChangchunChina
  2. 2.State Environmental Protection Key Laboratory of Wetland Ecology and Vegetation RestorationNortheast Normal UniversityChangchunChina
  3. 3.Department of Ecosystem Science and ManagementThe Pennsylvania State UniversityUniversity ParkUSA

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