Comparative morphology of in vivo and in vitro laticiferous cells and potential use of in vitro laticifers in early selection of rubber tree clones
- 30 Downloads
The laticifer density in callus of young tissue is positively correlated with the rubber yields of adult trees and can serve as an early selection marker to accelerate rubber tree breeding.
The rubber tree (Hevea brasiliensis Muell. Arg.) is a perennial crop with a breeding cycle of approximately 30 years in the conventional breeding practices. The lack of suitable parameters linking the juvenile and adult trees has made the early selection attempts inefficient. We performed comparative morphology of laticifer cells in callus induced from young shoots and the primary and secondary laticifers in the trunk of adult trees, and investigated their relationships to rubber yields. Results indicate that the secondary laticifers have smooth lateral walls and perforated end walls and belong to articulated anastomosing laticifers. The primary laticifers are unbranched, non-articulated, and anastomosing, and have bumpy lateral walls when matured. The callus-derived laticifers are morphologically similar to the secondary laticifers with smooth lateral walls and the existence of end walls, and similar relative cell wall thickness. The laticifer density in callus and the laticifer ring number in bark are both genotype-dependent, and they are both highly correlated with the rubber yields with correlation coefficients R = 0.846 (P < 0.002) and R = 0.899 (P < 0.002) for the callus and bark, respectively. Since callus can be induced from tissues of young seedlings in cross-pollinated populations, the laticifer density in callus can serve as an early selection marker of seedlings for high rubber yields and may be used to shorten the rubber tree breeding cycle.
KeywordsCallus-aided breeding Early selection marker Anastomosing Articulated Non-articultated Laticifer
This research was supported by the Natural Science Foundation of China (31471561).
- Bobilioff W (1919) Investigations concerning the origin of latex vessels and latex in Hevea brasiliensis. Arch v d Rubberc 3:43–46Google Scholar
- British Standard (1972) British standard methods of testing natural rubber latices. BS 1672, pp 8–9Google Scholar
- Eschbach JM, Roussel D, van de Sype H, Jacob JL, D’Auzac J (1984) Relationship between yield and clonal physiological characteristics of latex from Hevea brasiliensis. Physiologie Vegetale 22:295–304Google Scholar
- Gomez JB (1982) Anatomy of Hevea and its influence on latex production. Malaysian Rubber Research and Development Board, Kuala LumpurGoogle Scholar
- Guo XY, Zhou ZY, Zhan SR (1995) The second validation report of early yield prediction in Hevea brasiliensis. Chin J Trop Agric 15:9–13Google Scholar
- Huat OS (1981) Correlations between yield, girth and bark thickness of RRIM clone trials. J Rubber Res Inst Malays 29:1–14Google Scholar
- Milanez FR (1946) Nota prévia sobre os laticíferos de Hevea brasiliensis. Arquivos do Serviço Florestal 2:39–65Google Scholar
- Nugawela A, Aluthhewage RK (1985) Gas exchange parameters for early selection of Hevea brasiliensis Muell. Arg. J Rubber Res Inst Sri Lanka 1:13–20Google Scholar
- Rudall PJ (1987) Laticifer in Euphorbiaceae—a conspectus. Bot J 94:143–163Google Scholar
- Sreelatha S, Jacob JL, Mercykutty VC, Simon SP, Krishnakumar R (2014) ATP concentration in latex as an indicator for early evaluation of yield in Hevea brasiliensis. Jpn Circ J 48:698–704Google Scholar
- Wu ZL, Tan HY, Zeng RZ, Hao BZ (2000) Primary laticifer differentiation of Hevea brasiliensis in relation to shoot growth. Chin J Trop Crops 21:1–6Google Scholar
- Wu JL, Hao BZ, Tan HY (2002) Wound-induced laticifer differentiation in Hevea brasiliensis shoots mediated by jasmonic acid. J Rubber Res 5:53–63Google Scholar
- Yu J, Zeng X, Yang S, Huang H, Tian W (2008a) Relationship between rate of laticifer differentiation, number of laticifer rows and rubber yield among 1981 irrdb germplasm collections of Hevea brasiliensis. J Rubber Res 11:43–51Google Scholar
- Yu JH, Zeng X, Yang SG, Huang HS, Tian WM (2008b) Relationship between rate of laticifer differentiation,number of laticifer rows and rubber yield among 1981 IRRDB germplasm collections of Hevea brasiliensis. J Rubber Res 11:43–51Google Scholar
- Zeng X, Hu YS, Huang HS, Fang JL (2006) The evaluation report of 1981’IRRDB rubber tree germplasm—1987-field-planted germplasm. Chin J Trop Crop 27:34–38Google Scholar
- Zhao XQ (1987) The significance of the structure of laticifer with relation to the exudation of latex in Hevea brasiliensis. J Nat Rubber Res 2:94–98Google Scholar
- Zhou ZY, Guo QY, Zhan SR, Liu JX (1994) Early yield predicting for hevea seedlings from hand-pollination. Chin J Trop Crops 15:1–9Google Scholar