Margin diagnosis for endoscopic submucosal dissection of early gastric cancer using multiphoton microscopy

  • Xiaoling Zheng
  • Ning Zuo
  • Hongxin Lin
  • Liqin Zheng
  • Ming Ni
  • Guizhu WuEmail author
  • Jianxin Chen
  • Shuangmu ZhuoEmail author


Background and Aims

Endoscopic submucosal dissection (ESD) has become the primary option for the treatment of early gastric cancer (EGC). Thus, it is necessary to diagnose whether residual cancer cells exist in the ESD specimen margins, which can affect tumor recurrence and survival rates in the future. Multiphoton microscopy (MPM) can be suitably used for nondestructive imaging of biological tissue on a cellular level to enable real-time guidance during endoscopic therapy. Considering this, the objective of this study is to explore the practicality of MPM for the diagnosis of ESD specimen margins in the case of EGC.


First, a total of 20 surgical samples was imaged using the proposed MPM technique to obtain two-photo excited fluorescence signal from the intrinsic fluorescent substances within cells and second-harmonic generation signal from collagen; these signals were used to determine MPM pathological features for margin diagnosis. Then, a double-blind study of 50 samples was conducted to evaluate the diagnosis results based on the obtained MPM pathological features.


Multiphoton microscopy can accurately identify the cytological and morphological differences between tissue in the negative and positive margin. The sensitivity, specificity, accuracy, negative predictive, and positive predictive values of MPM in the diagnosis of ESD specimen margins were 97.62, 75.00, 94.00, 95.35, and 85.71%, respectively.


These results indicate that MPM can be used as an effective, real-time, and label-free novel method to determine intraoperative resection margins.


Multiphoton microscopy Early gastric cancer Resection margin ESD 



This project was supported by the National Natural Science Foundation of China (81771881), the National Key Basic Research Program of China (2015CB352006), the Program for Changjiang Scholars and Innovative Research Team in University (IRT_15R10), the Natural Science Foundation of Fujian Province (2018J07004, 2016J01433, 2018J01170, and 2018J01784), the Special Funds of the Central Government Guiding Local Science and Technology Development (2017L3009), the Science and Technology Planning Project of Guangdong Province (2016A020220014), and the Fujian Provincial Youth Top-notch Talent Support Program.


Xiaoling Zheng, Ning Zou, Hongxin Lin, Liqin Zheng, Ming Ni, Guizhu Wu, Jianxin Chen, and Shuangmu Zhuo, have no conflicts of interest or financial ties to disclose.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Endoscopy, Fujian Provincal Hospital, Fujian Medical University & College of Photonic and Electronic EngineeringFujian Normal UniversityFuzhouChina
  2. 2.Fujian Provincial Key Laboratory for Photonics Technology & Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of EducationFujian Normal UniversityFuzhouChina
  3. 3.School of Biological Sciences & EngineeringYachay Tech UniversitySan Miguel de UrcuquíEcuador
  4. 4.Shanghai First Maternity and Infant HospitalTongji University School of MedicineShanghaiChina

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