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A novel endoscopic fluorescent band ligation method for tumor localization

Abstract

Background

Accurate tumor localization is essential for minimally invasive surgery. This study describes the development of a novel endoscopic fluorescent band ligation method for the rapid and accurate identification of tumor sites during surgery.

Methods and materials

The method utilized a fluorescent rubber band, made of indocyanine green (ICG) and a liquid rubber solution mixture, as well as a near-infrared fluorescence laparoscopic system with a dual light source using a high-powered light-emitting diode (LED) and a 785-nm laser diode. The fluorescent rubber bands were endoscopically placed on the mucosae of porcine stomachs and colons. During subsequent conventional laparoscopic stomach and colon surgery, the fluorescent bands were assayed using the near-infrared fluorescence laparoscopy system.

Results

The locations of the fluorescent clips were clearly identified on the fluorescence images in real time. The system was able to distinguish the two or three bands marked on the mucosal surfaces of the stomach and colon. Resection margins around the fluorescent bands were sufficient in the resected specimens obtained during stomach and colon surgery.

Conclusion

These novel endoscopic fluorescent bands could be rapidly and accurately localized during stomach and colon surgery. Use of these bands may make possible the excision of exact target sites during minimally invasive gastrointestinal surgery.

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Acknowledgments

This research was supported by the National Research Foundation of Korea (NRF), funded by the Ministry of Science, ICT & Future Planning (2014-030120), and by a Grant from the National Cancer Center (NCC-1510150).

Author information

Correspondence to Dae Kyung Sohn.

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Disclosure

All authors (Hyun JH, Kim S-K, Kim KG, Kim HR, Lee HM, Park S, Kim SC, Choi Y, and Sohn DK) have no conflicts of interest or financial ties to disclose.

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Supplementary material 1 (WMV 14064 kb)

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Hyun, J.H., Kim, S., Kim, K.G. et al. A novel endoscopic fluorescent band ligation method for tumor localization. Surg Endosc 30, 4659–4663 (2016) doi:10.1007/s00464-016-4785-1

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Keywords

  • Tumor localization
  • Minimal invasive surgery
  • Fluorescence
  • Endoscopy