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Comparison of the effects of gasless procedure, CO2-pneumoperitoneum, and laparotomy on splenic and hepatic natural killer activity in a rat model

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Abstract

Background: Natural killer (NK) activity plays an important role in the prevention of tumor metastasis. However, few studies compared changes in NK activities between gas(less) laparoscopy and open surgery. Materials and methods: Donryu rats were randomized to 4 groups (n = 6–9, for each lime point in each group): gasless group, pneumoperitoneum group, laparotomy group, and control group. The surgical treatment groups received additional cecal resection at the end of the procedures. Spleen cells and hepatic nonparenchymal cells were harvested at 6, 24, or 96 h, to determine the splenic and hepatic NK activities against YAC-1 cells. Results: Compared with the anesthesia control, laparotomy and pneumoperitoneum depressed splenic NK activity at 24 h (p < 0.01 for laparotomy and p < 0.05, for pneumoperitoneum) and 96 h (p < 0.05). Laparotomy also depressed hepatic NK activity at 24 h (p < 0.05). In contrast, the gasless procedure did not show any deterioration in the splenic and hepatic NK activities. Conclusion: These results suggest that gasless laparoscopic surgery would be a favorable approach in terms of the preservation of NK activities.

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Acknowledgments

We thank Dr. Kouki Jinnai, Vice President, Senboku Jinnai Hospital, for his kind advice.

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Correspondence to I. Takeuchi.

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Takeuchi, I., Ishida, H., Mori, T. et al. Comparison of the effects of gasless procedure, CO2-pneumoperitoneum, and laparotomy on splenic and hepatic natural killer activity in a rat model . Surg Endosc 18, 255–260 (2004). https://doi.org/10.1007/s00464-002-9180-4

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Keywords

  • Natural killer activity
  • Large granular lymphocyte
  • Pneumoperitoneum
  • Gasless laparoscopy
  • Laparotomy