Bioprocess and Biosystems Engineering

, Volume 43, Issue 2, pp 261–272 | Cite as

Advanced oxidation processes applied for color removal of textile effluent using a home-made peroxidase from rice bran

  • Natalia Klanovicz
  • Aline Frumi Camargo
  • Fábio Spitza Stefanski
  • Jessica Zanivan
  • Thamarys Scapini
  • Rafaela Pollon
  • Andressa Warken
  • Letícia Paliga
  • Karina Paula Preczeski
  • Anderson André Genro Alves Ribeiro
  • Jaqueline Garda-Buffon
  • Gislaine Fongaro
  • Helen TreichelEmail author
Research Paper


Enzymes are becoming tools in industrial processes because of several advantages, including activity in mild environmental conditions, and high specificity. Peroxidase, for one, stably oxidizes several substrates. The present study aimed to develop advanced oxidation processes (AOP), using non-commercial rice bran peroxidase to remove color and toxicity of synthetic textile wastewater. Using a microwave and shaker system, we obtained 38.9% and 100% of effluent color removal after peroxidase treatment, respectively. In addition, the shaker system decants residual dye particles through filtration, providing the textile industry with an economical and environmentally viable alternative to effluent treatment. In toxicity tests results, both treatment systems damaged the used genetic material. This damage occurs because of industrial discharge of wastewater into water bodies; effluent dilution reduced this damage. The data suggest that peroxidase as a textile effluent treatment has potential uses in industrial processes, because rice bran peroxidase has demonstrated affinity with dyes.


Enzymatic precipitation Shaker system Microwave radiation Toxicity test 



CAPES, CAPES-PNPD, CNPq, and FAPERGS for the financial support of this study.

Compliance with ethical standards

Conflict of interest

The authors report no interest conflict.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Natalia Klanovicz
    • 1
  • Aline Frumi Camargo
    • 1
  • Fábio Spitza Stefanski
    • 1
  • Jessica Zanivan
    • 1
  • Thamarys Scapini
    • 1
  • Rafaela Pollon
    • 1
  • Andressa Warken
    • 1
  • Letícia Paliga
    • 1
  • Karina Paula Preczeski
    • 1
  • Anderson André Genro Alves Ribeiro
    • 1
  • Jaqueline Garda-Buffon
    • 2
  • Gislaine Fongaro
    • 3
  • Helen Treichel
    • 1
    Email author
  1. 1.Laboratory of Microbiology and BioprocessFederal University of Fronteira SulErechimBrazil
  2. 2.Escola de Química E AlimentosUniversidade Federal de Rio GrandeRio GrandeBrazil
  3. 3.Laboratory of VirologyFederal University of Santa CatarinaFlorianópolisBrazil

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