Advertisement

Comparing impacts of physicochemical properties and hydrolytic inhibitors on enzymatic hydrolysis of sugarcane bagasse

  • Mingfu Li
  • Chenyan Guo
  • Bin Luo
  • Changzhou Chen
  • Shuangfei Wang
  • Douyong MinEmail author
Research Paper
  • 31 Downloads

Abstract

An autohydrolysis pretreatment with different conditions was applied to sugarcane bagasse to compare the impacts of the physicochemical properties and hydrolytic inhibitors on its enzymatic hydrolysis. The results indicate that the autohydrolysis conditions significantly affected the physicochemical properties and inhibitors, which further affected the enzymatic hydrolysis. The inhibitor amount, pore size, and crystallinity degree increased with increasing autohydrolysis severity. Furthermore, the enzymatic hydrolysis was enhanced with increasing severity owing to the removal of hemicellulose and lignin. The physicochemical obstruction impeded the enzymatic hydrolysis more than the inhibitors. The multivariate correlated component regression analysis enabled an evaluation of the correlations between the physicochemical properties (and inhibitors) and enzymatic hydrolysis for the first time. According to the results, an autohydrolysis with a severity of 4.01 is an ideal pretreatment for sugarcane bagasse for sugar production.

Keywords

Sugarcane bagasse Autohydrolysis Enzymatic hydrolysis Physicochemical properties Inhibitors 

Notes

Acknowledgements

This project was funded by the Innovation Project of Guangxi Graduate Education (YCBZ2019017), Guangxi Natural Science Foundation (2018JJA130224), and Guangxi Key Laboratory of Clean Pulp and Papermaking and Pollution Control Foundation (ZR201805-7).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

References

  1. 1.
    Allen SA, Clark W, McCaffery JM et al (2010) Furfural induces reactive oxygen species accumulation and cellular damage in Saccharomyces cerevisiae. Biotechnol Biofuels 3:2–12CrossRefPubMedPubMedCentralGoogle Scholar
  2. 2.
    Isis Amores IBPM, Sáez GMMB (2013) Ethanol production from sugarcane bagasse pretreated by steam explosion. Electron J Energy Environ 1:25–36Google Scholar
  3. 3.
    Ko JK, Kim Y, Ximenes E (2015) Ladisch MR et al Effect of liquid hot water pretreatment severity on properties of hardwood lignin and enzymatic hydrolysis of cellulose. Biotechnol Bioeng 112:252–262CrossRefPubMedGoogle Scholar
  4. 4.
    Hendriks ATWM, Zeeman G (2009) Pretreatments to enhance the digestibility of lignocellulosic biomass. Bioresour Technol 100:10–18CrossRefPubMedGoogle Scholar
  5. 5.
    Canilha L, Chandel AK, Dos Santos Suzane, Milessi T et al (2012) Bioconversion of sugarcane biomass into ethanol: an overview about composition, pretreatment methods, detoxification of hydrolysates, enzymatic saccharification, and ethanol fermentation. J Biomed Biotechnol 2012:1–15CrossRefGoogle Scholar
  6. 6.
    Kaar WE, Gutierrez CV, Kinoshita CM (1998) Steam explosion of sugarcane bagasse as pretreatment for conversion to ethanol. Biomass Bioenerg 3:277–287CrossRefGoogle Scholar
  7. 7.
    Saha BC, Cotta MA (2011) Continuous ethanol production from wheat straw hydrolysate by recombinant ethanologenic Escherichia coli strain FBR5. Appl Microbiol Biotechnol 90:477–487CrossRefPubMedGoogle Scholar
  8. 8.
    Iroba KL, Tabil LG, Dumonceaux T et al (2013) Effect of alkaline pretreatment on chemical composition of lignocellulosic biomass using radio frequency heating. Biosyst Eng 116:385–398CrossRefGoogle Scholar
  9. 9.
    Sindhu R, Binod P, Pandey A (2016) Biological pretreatment of lignocellulosic biomass—an overview. Bioresour Technol 199:76–82CrossRefPubMedGoogle Scholar
  10. 10.
    Shevchenko SM, Chang K, Robinson J et al (2000) Optimization of monosaccharide recovery by post-hydrolysis of the water-soluble hemicellulose component after steam explosion of softwood chips. Bioresour Technol 72:207–211CrossRefGoogle Scholar
  11. 11.
    Bezerra TL, Ragauskas AJ (2016) A review of sugarcane bagasse for second-generation bioethanol and biopower production. Biofuels Bioprod Biorefining 5(10):634–647CrossRefGoogle Scholar
  12. 12.
    Martin C, Galbe M, Nilvebrant NO et al (2002) Comparison of the fermentability of enzymatic hydrolyzates of sugarcane bagasse pretreated by steam explosion using different impregnating agents. Appl Biochem Biotechnol 98–100:699–716CrossRefPubMedGoogle Scholar
  13. 13.
    Vallejos ME, Felissia FE, Kruyeniski J et al (2015) Kinetic study of the extraction of hemicellulosic carbohydrates from sugarcane bagasse by hot water treatment. Ind Crops Prod 67:1–6CrossRefGoogle Scholar
  14. 14.
    Kim JS, Lee YY, Kim TH (2016) A review on alkaline pretreatment technology for bioconversion of lignocellulosic biomass. Bioresour Technol 199:42–48CrossRefPubMedGoogle Scholar
  15. 15.
    Sindhu R, Kuttiraja M, Binod P, Sukumaran RK, Pandey A et al (2011) Dilute acid pretreatment and enzymatic saccharification of sugarcane tops for bioethanol production. Bioresour Technol 102:10915–10921CrossRefPubMedGoogle Scholar
  16. 16.
    Zhou Z, Cheng Y, Zhang W et al (2016) Characterization of lignins from sugarcane bagasse pretreated with green liquor combined with ethanol and hydrogen peroxide. BioResources 11:3191–3203Google Scholar
  17. 17.
    Hashmi M, Sun Q, Tao J et al (2017) Comparison of autohydrolysis and ionic liquid 1-butyl-3-methylimidazolium acetate pretreatment to enhance enzymatic hydrolysis of sugarcane bagasse. Bioresour Technol 224:714–720CrossRefPubMedGoogle Scholar
  18. 18.
    Lee JM, Shi J, Venditti RA et al (2009) Autohydrolysis pretreatment of coastal bermuda grass for increased enzyme hydrolysis. Bioresour Technol 100:6434–6441CrossRefPubMedGoogle Scholar
  19. 19.
    Ko JK, Um Y, Park Y et al (2015) Compounds inhibiting the bioconversion of hydrothermally pretreated lignocellulose. Appl Microbiol Biotechnol 99:4201–4212CrossRefPubMedGoogle Scholar
  20. 20.
    Hodge DB, Karim MN, Schell DJ et al (2008) Soluble and insoluble solids contributions to high-solids enzymatic hydrolysis of lignocellulose. Biores Technol 99:8940–8948CrossRefGoogle Scholar
  21. 21.
    Ximenes E, Kim Y, Mosier N et al (2010) Inhibition of cellulases by phenols. Enzyme Microb Technol 46:170–176CrossRefGoogle Scholar
  22. 22.
    Kim Y, Ximenes E, Mosier NS et al (2011) Soluble inhibitors/deactivators of cellulase enzymes from lignocellulosic biomass. Enzyme Microb Technol 48:408–415CrossRefPubMedGoogle Scholar
  23. 23.
    Qing Q, Yang B, Wyman CE (2010) Xylooligomers are strong inhibitors of cellulose hydrolysis by enzymes. Bioresour Technol 101:9624–9630CrossRefPubMedGoogle Scholar
  24. 24.
    Michelin M, Ximenes E, de Moraes MDLT et al (2016) Effect of phenolic compounds from pretreated sugarcane bagasse on cellulolytic and hemicellulolytic activities. Bioresour Technol 199:275–278CrossRefPubMedGoogle Scholar
  25. 25.
    Ghose TK (1987) Measurement of cellulase activities. Pure Appl Chem 59:257–268CrossRefGoogle Scholar
  26. 26.
    Min D, Xu R, Hou Z et al (2015) Minimizing inhibitors during pretreatment while maximizing sugar production in enzymatic hydrolysis through a two-stage hydrothermal pretreatment. Cellulose 22:1253–1261CrossRefGoogle Scholar
  27. 27.
    Sluiter A, Hames B, Ruiz R, Scarlata C, Sluiter J, Templeton D, Crocker D (2012) Determination of structural carbohydrates and lignin in biomass. Laboratory Analytical Procedure, National Renewable Laboratory, NREL/TP-510-42618, USAGoogle Scholar
  28. 28.
    Nitsos CK, Choli-Papadopoulou T, Matis KA et al (2016) Optimization of hydrothermal pretreatment of hardwood and softwood lignocellulosic residues for selective hemicellulose recovery and improved cellulose enzymatic hydrolysis. ACS Sustain Chem Eng 4:4529–4544CrossRefGoogle Scholar
  29. 29.
    Min D, Wei L, Zhao T et al (2018) Combination of hydrothermal pretreatment and sodium hydroxide post-treatment applied on wheat straw for enhancing its enzymatic hydrolysis. Cellulose 25:1197–1206CrossRefGoogle Scholar
  30. 30.
    Phillips RB, Jameel H, Chang HM (2013) Integration of pulp and paper technology with bioethanol production. Biotechnol Biofuels 6:13–13CrossRefPubMedPubMedCentralGoogle Scholar
  31. 31.
    Michelin M, Ximenes E, De Moraes Teixeira, Polizeli MDL et al (2016) Effect of phenolic compounds from pretreated sugarcane bagasse on cellulolytic and hemicellulolytic activities. Bioresour Technol 199:275–278CrossRefPubMedGoogle Scholar
  32. 32.
    Hu F, Jung S, Ragauskas A (2012) Pseudo-lignin formation and its impact on enzymatic hydrolysis. Bioresour Technol 117:7–12CrossRefPubMedGoogle Scholar
  33. 33.
    Hu F, Ragauskas A (2014) Suppression of pseudo-lignin formation under dilute acid pretreatment conditions. RSC Adv 4:4317–4323CrossRefGoogle Scholar
  34. 34.
    Zhao X, Zhang L, Liu D (2012) Biomass recalcitrance. Part I: the chemical compositions and physical structures affecting the enzymatic hydrolysis of lignocellulose. Biofuels Bioprod Biorefining 6:465–482CrossRefGoogle Scholar
  35. 35.
    Garrote G, Domínguez H, Parajó JC (1999) Hydrothermal processing of lignocellulosic materials. Eur J Wood Wood Prod 57:191–202CrossRefGoogle Scholar
  36. 36.
    Jonsson LJ, Alriksson B, Nilvebrant NO (2013) Bioconversion of lignocellulose: inhibitors and detoxification. Biotechnol Biofuels 6:16CrossRefPubMedPubMedCentralGoogle Scholar
  37. 37.
    Batalha LAR, Han Q, Jameel H et al (2015) Production of fermentable sugars from sugarcane bagasse by enzymatic hydrolysis after autohydrolysis and mechanical refining. Biores Technol 180:97–105CrossRefGoogle Scholar
  38. 38.
    Ju X, Grego C, Zhang X (2013) Specific effects of fiber size and fiber swelling on biomass substrate surface area and enzymatic digestibility. Biores Technol 144:232–239CrossRefGoogle Scholar
  39. 39.
    Zhang YP, Lynd LR (2004) Toward an aggregated understanding of enzymatic hydrolysis of cellulose: noncomplexed cellulase systems. Biotechnol Bioeng 88:797–824CrossRefPubMedGoogle Scholar
  40. 40.
    Kumar L, Arantes V, Chandra R et al (2012) The lignin present in steam pretreated softwood binds enzymes and limits cellulose accessibility. Bioresour Technol 103:201–208CrossRefPubMedGoogle Scholar
  41. 41.
    Ju X, Engelhard M, Zhang X (2013) An advanced understanding of the specific effects of xylan and surface lignin contents on enzymatic hydrolysis of lignocellulosic biomass. Bioresour Technol 132:137–145CrossRefPubMedGoogle Scholar
  42. 42.
    Zheng Y, Zhang S, Miao S et al (2013) Temperature sensitivity of cellulase adsorption on lignin and its impact on enzymatic hydrolysis of lignocellulosic biomass. J Biotechnol 166:135–143CrossRefPubMedGoogle Scholar
  43. 43.
    Ding D, Li P, Zhang X (2019) Synergy of hemicelluloses removal and bovine serum albumin blocking of lignin for enhanced enzymatic hydrolysis. Bioresour Technol 273:231–236CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.College of Light Industry and Food EngineeringGuangxi UniversityNanningPeople’s Republic of China
  2. 2.Guangxi Key Laboratory of Clean Pulp and Papermaking and Pollution ControlNanningPeople’s Republic of China

Personalised recommendations