Comparing impacts of physicochemical properties and hydrolytic inhibitors on enzymatic hydrolysis of sugarcane bagasse

  • Mingfu Li
  • Chenyan Guo
  • Bin Luo
  • Changzhou Chen
  • Shuangfei Wang
  • Douyong MinEmail author
Research Paper


An autohydrolysis pretreatment with different conditions was applied to sugarcane bagasse to compare the impacts of the physicochemical properties and hydrolytic inhibitors on its enzymatic hydrolysis. The results indicate that the autohydrolysis conditions significantly affected the physicochemical properties and inhibitors, which further affected the enzymatic hydrolysis. The inhibitor amount, pore size, and crystallinity degree increased with increasing autohydrolysis severity. Furthermore, the enzymatic hydrolysis was enhanced with increasing severity owing to the removal of hemicellulose and lignin. The physicochemical obstruction impeded the enzymatic hydrolysis more than the inhibitors. The multivariate correlated component regression analysis enabled an evaluation of the correlations between the physicochemical properties (and inhibitors) and enzymatic hydrolysis for the first time. According to the results, an autohydrolysis with a severity of 4.01 is an ideal pretreatment for sugarcane bagasse for sugar production.


Sugarcane bagasse Autohydrolysis Enzymatic hydrolysis Physicochemical properties Inhibitors 



This project was funded by the Innovation Project of Guangxi Graduate Education (YCBZ2019017), Guangxi Natural Science Foundation (2018JJA130224), and Guangxi Key Laboratory of Clean Pulp and Papermaking and Pollution Control Foundation (ZR201805-7).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.College of Light Industry and Food EngineeringGuangxi UniversityNanningPeople’s Republic of China
  2. 2.Guangxi Key Laboratory of Clean Pulp and Papermaking and Pollution ControlNanningPeople’s Republic of China

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