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A versatile Pseudomonas putida KT2440 with new ability: selective oxidation of 5-hydroxymethylfurfural to 5-hydroxymethyl-2-furancarboxylic acid

  • Qianqian Xu
  • Zhaojuan Zheng
  • Lihua Zou
  • Chen Zhang
  • Fen Yang
  • Kaijie Zhou
  • Jia OuyangEmail author
Research Paper

Abstract

Currently, biotransformation of 5-hydroxymethylfurfural (HMF) into a series of high-value bio-based platform chemicals is massively studied. In this study, selective biooxidation of HMF to 5-hydroxymethyl-2-furancarboxylic acid (HMFCA) by Pseudomonas putida KT2440 with superior titer, yield, and productivity was reported. The biocatalytic performances of P. putida KT2440 were optimized separately. Under optimal conditions, 100% yield of HMFCA was obtained when HMF concentration was less than 150 mM, while the maximum concentration of 155 mM was achieved from 160 mM HMF in 12 h. P. putida KT2440 was highly tolerate to HMF, up to 190 mM. Besides, it was capable of selective oxidation of other furan aldehydes to the corresponding carboxylic acids with good yield of 100%. This study further demonstrates the potential of P. putida KT2440 as a biocatalyst for biomass conversion, as this strain has been proved the capacity to convert and utilize many kinds of biomass-derived sugars and ligin-derived aromatic compounds.

Keywords

Whole-cell biocatalysis 5-Hydroxymethylfurfural 5-Hydroxymethyl-2-furancarboxylic acid Pseudomonas putida KT2440 Selective oxidation 

Notes

Acknowledgements

This study was supported by the National Natural Science Foundation of China (51776099).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Qianqian Xu
    • 1
    • 2
  • Zhaojuan Zheng
    • 1
    • 3
    • 4
  • Lihua Zou
    • 1
    • 3
  • Chen Zhang
    • 1
    • 3
  • Fen Yang
    • 3
  • Kaijie Zhou
    • 3
  • Jia Ouyang
    • 1
    • 3
    • 4
    Email author
  1. 1.Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest ResourcesNanjing Forestry UniversityNanjingPeople’s Republic of China
  2. 2.College of ForestryNanjing Forestry UniversityNanjingPeople’s Republic of China
  3. 3.College of Chemical EngineeringNanjing Forestry UniversityNanjingPeople’s Republic of China
  4. 4.Key Laboratory of Forestry Genetics & Biotechnology (Nanjing Forestry University), Ministry of EducationNanjingPeople’s Republic of China

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