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Bioprocess and Biosystems Engineering

, Volume 42, Issue 10, pp 1647–1659 | Cite as

Optimization of free fatty acid production by enzymatic hydrolysis of vegetable oils using a non-commercial lipase from Geotrichum candidum

  • Matheus M. Ferreira
  • Gustavo F. de Oliveira
  • Rodrigo C. Basso
  • Adriano A. Mendes
  • Daniela B. HirataEmail author
Research Paper
  • 192 Downloads

Abstract

This study aimed to optimize free fatty acid production by enzymatic hydrolysis of cottonseed, olive and palm kernel oils in stirred-tank reactors using a lipase from Geotrichum candidum (GCL-I). The effect of pH, temperature and substrate concentration on the hydrolytic activity of GCL-I using these vegetable oils was investigated. Thermal stability tests and thermodynamic studies were also performed. A complete hydrolysis of cottonseed oil was obtained after 120 min of reaction, while for olive and palm kernel maximum hydrolysis percentage was 96.4% and 60.1%, respectively. GCL-I exhibited the highest activity in the hydrolysis of vegetable oils that are rich in unsaturated-fatty acids (cottonseed and olive oils). Under optimal conditions (46.8% m/m of oil, 6.6 U/g of the reaction mixture at 40 °C), complete cottonseed oil hydrolysis was observed at 60 min of reaction performed in an emulsifier-free system with no buffer.

Keywords

Lipase specificity Free fatty acid production Kinetic Thermodynamic Optimization 

Notes

Acknowledgements

This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior-Brasil (CAPES)-Finance Code 001. The authors are grateful to Fundação de Amparo à Pesquisa do Estado de Minas Gerais-FAPEMIG (TEC-APQ-02755-16) for its financial support. MSc. Matheus M. Ferreira thanks CAPES for his student fellowship. Gustavo F. Oliveira thanks Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) for his IC fellowship.

Compliance with ethical standards

Conflict of interest

The authors report no conflict of interest.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Matheus M. Ferreira
    • 1
  • Gustavo F. de Oliveira
    • 2
  • Rodrigo C. Basso
    • 1
  • Adriano A. Mendes
    • 2
  • Daniela B. Hirata
    • 1
    • 2
    Email author
  1. 1.Graduate Program in Chemical EngineeringFederal University of AlfenasPoços de CaldasBrazil
  2. 2.Institute of ChemistryFederal University of AlfenasAlfenasBrazil

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