Immobilized enzyme on pulp fiber through layer-by-layer technique using cationic polyacrylamide for whitewater treatment from papermaking
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Anionic pectic substances in whitewater from papermaking are detrimental to machine operation and product quality. Pectinase was immobilized on pulp fiber using cationic polyacrylamide with layer-by-layer method to obtain bound enzyme with tunable activity and good performance for wastewater treatment. It was revealed that high charge density and low molecular weight for cationic polyacrylamide were advantageous for enzymatic activity. During the layer-by-layer adsorption process, the enzymatic activity of the immobilized enzyme increased nearly linearly with the layer number from 983 to 3074 U/g until the fourth layer. The stability of the four-layer immobilized enzyme was improved. The multilayer immobilized enzyme exhibited good reusability and storage stability compared with monolayer enzyme. At dosage of 10 U/mL, the cationic demand of the whitewater samples was reduced by 15% using four-layer immobilized enzyme. The results indicated a potential route to prepare immobilized enzyme with good performance for wastewater treatment in papermaking industry.
KeywordsEnzyme immobilization Pulp fiber Layer-by-layer Papermaking Cationic polyacrylamide
This work was supported by State Key Laboratory of Pulp and Paper Engineering (201502), Innovation fund for young teachers of Tianjin University of Science and Technology (2016LG19), and Key project of Tianjin Natural Science Foundation (16JCZDJC39700).
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Conflict of interest
The authors declare that they have no conflict of interest.
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