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Production and characterization of glycolipid biosurfactant from Achromobacter sp. (PS1) isolate using one-factor-at-a-time (OFAT) approach with feasible utilization of ammonia-soaked lignocellulosic pretreated residues

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Abstract

With the ever growing increase in the demands of biosurfactants, the present study was focused in developing a set of parameters influencing biosurfactant production using one-factor-at-a-time (OFAT) approach in chemically defined medium from an indigenous isolate of Achromobacter sp. (PS1). Subsequently, the feasibility of biosurfactant production was examined using influential OFAT parameters in same medium, replacing only carbon source with lignocellulosic hydrolyzed sugars. These sugars were obtained from ammonia (15% v/v) soaking pretreatment of lignocellulosic residues (7.5% solid loading at 70 °C for 72 h) with subsequent saccharification using lignocellulolytic enzymes. OFAT influential parameters observed were dextrose (3–4% w/v); C/N ratio 8.3 using sodium nitrate and beef extract; 2 × 10−5 grams equivalents Fe2+; 1500 mM PO43− in minimal salt medium (MSM) at pH 7.0, 120 rpm, 30 °C resulting in 4.13 ± 0.12 g/L rhamnolipid in 192 h with 30.42 mN/m surface tension and 136 mg/L critical micelle concentration (CMC). Biosurfactant was characterized using tandem-MS and NMR as rhamnolipid with six-congeners, Rha-C10–C10 and Rha-Rha-C10-C10 being the most abundant. Rhamnolipid showed broad range stability at temperatures (30–121 °C), pH (6–12), and salinity (0.5–5% w/v) of NaCl. In Rice-straw (RS) hydrolysate, maximum glucan (73.10%) and xylan (91.13%) were obtained and the RS-hydrolysate medium with a total of 4.55% (w/v) sugars under optimum OFAT parameters (other than dextrose) showed at par production of 3.55 ± 0.06 g/L of rhamnolipid in 192 h with YBS/S (biosurfactant yield per gram of sugar consumed) of 0.08 g/g and YBS/CDW (biosurfactant yield per gram of cell biomass) of 0.68 g/g.

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Abbreviations

OFAT:

One-factor-at-a-time

RS:

Rice-straw

WS:

Wheat-straw

SB:

Sugarcane-bagasse

AAS:

Aqueous ammonia soaking

MSM:

Minimal salt medium

CMC:

Critical micelle concentration

CDW:

Cell dry weight

RL:

Rhamnolipid

ASTM:

American Society for Testing and Materials

References

  1. 1.

    Joy S, Rahman PKSM, Sharma S (2017) Biosurfactant production and concomitant hydrocarbon degradation potentials of bacteria isolated from extreme and hydrocarbon contaminated environments. Chem Eng J 317:232–241

  2. 2.

    Klosowska-Chomiczewska IE, Medrzycka K, Hallmann E, Karpenko E, Pokynbroda T, Macierzanka A, Jungnickel C (2017) Rhamnolipid CMC prediction. J Colloid Interface Sci 488:10–19

  3. 3.

    Gudina EJ, Rodrigues AI, De Freitas V, Azevedo Z, Teixeira JA, Rodrigues LR (2016) Valorization of agro-industrial wastes towards the production of rhamnolipids. Bioresour Technol 212:144–150

  4. 4.

    Zambry NS, Ayoib A, Noh NA, Yahya AR (2017) Production and partial characterization of biosurfactant produced by Streptomyces sp. R1. Bioprocess Biosyst Eng 40:1007–1016

  5. 5.

    Varjani SJ, Upasani VN (2017) Critical review on biosurfactant analysis, purification and characterization using rhamnolipid as a model biosurfactant. Bioresour Technol 232:389–397

  6. 6.

    Grand market insights Research, Biosurfactants Market worth over $2.7 bn by 2024. [Online]. Global Market Insights, Inc. (2018). (Accessed 8 July 2018). Available: https://www.gminsights.com/pressrelease/biosurfactants-market-size

  7. 7.

    Singh R, Srivastava M, Shukla A (2016) Environmental sustainability of bioethanol production from rice straw in India: A review Renew Sustain Energy Rev 54:202–216

  8. 8.

    Capolupo L, Faraco V (2016) Green methods of lignocellulose pretreatment for biorefinery development. Appl Microbiol Biotechnol 100:9451–9467

  9. 9.

    Thompson DN, Campbell T, Bals B, Runge T, Teymouri F, Ovard LP (2013) Chemical preconversion: application of low-severity pretreatment chemistries for commoditization of lignocellulosic feedstock. Biofuels 4:323–340

  10. 10.

    Salvi DA, Aita GM, Robert D, Bazan V (2010) Dilute ammonia pretreatment of sorghum and its effectiveness on enzyme hydrolysis and ethanol fermentation. Appl Biochem Biotechnol 161:67–74

  11. 11.

    Rahman PKSM, Pasirayi G, Auger V, Ali Z (2010) Production of rhamnolipid biosurfactants by Pseudomonas aeruginosa DS10-129 in a microfluidic bioreactor. Biotechnol Appl Biochem 55:45–52

  12. 12.

    George S, Jayachandran K (2012) Production and characterization of rhamnolipid biosurfactant from waste frying coconut oil using a novel Pseudomonas aeruginosa D. J Appl Microbiol 114:373–383

  13. 13.

    Rikalovic MG, Gojgic-Cvijovic G, Vrvic MM, Karadzic I (2012) Production and characterization of rhamnolipids from Pseudomonas aeruginosa san ai. J Serb Chem Soc 77:27–42

  14. 14.

    Gunther NW, Nunez A, Fett W, Solaiman DK (2005) Production of rhamnolipids by Pseudomonas chlororaphis, a nonpathogenic bacterium. App Enviro Microbiol 71:2288–2293

  15. 15.

    Sluiter A, Hames B, Ruiz R, Scarlata C, Sluiter J, Templeton D, Crocker D (2008) Laboratory analytical procedure (LAP): determination of structural carbohydrates and lignin in biomass, National Renewable Energy Laboratory- NREL/TP-510-42618, 1617:1-16.

  16. 16.

    Selig M, Weiss N, Ji Y (2008) Laboratory analytical procedure (LAP): enzymatic saccharification of lignocellulosic biomass. National Renewable Energy Laboratory- NREL/TP-510-42629

  17. 17.

    Banat IM, Satpute SK, Cameotra SS, Patil R, Nyayanit NV (2014) Cost effective technologies and renewable substrates for biosurfactants production. Front microbiol 5:697

  18. 18.

    Ma KY, Sun MY, Dong W, He CQ, Chen FL, Ma YL (2016) Effects of nutrition optimization strategy on rhamnolipid production in a Pseudomonas aeruginosa strain DN1 for bioremediation of crude oil. Biocatal Agric Biotechnol 6:144–151

  19. 19.

    Ndlovu T, Rautenbach M, Khan S, Khan W (2017) Variants of lipopeptides and glycolipids produced by Bacillus amyloliquefaciens and Pseudomonas aeruginosa cultured in different carbon substrates. AMB Exp 7:109

  20. 20.

    Mukherjee S, Das P, Sivapathasekaran C, Sen R (2008) Enhanced production of biosurfactant by a marine bacterium on statistical screening of nutritional parameters. Biochem Eng J 42:254–260

  21. 21.

    Radzuan MN, Banat IM, Winterburn J (2017) Production and characterization of rhamnolipid using palm oil agricultural refinery waste. Bioresour Technol 225:99–105

  22. 22.

    Zhao F, Zhou J, Han S, Ma F, Zhang Y (2016) Medium factors on anaerobic production of rhamnolipids by Pseudomonas aeruginosa SG and a simplifying medium for in situ microbial enhanced oil recovery applications. World J Microbiol Biotechnol 32:54

  23. 23.

    Santos DKF, Rufino RD, Luna JM, Santos VA, Sarubbo LA (2016) Biosurfactants: multifunctional biomolecules of the 21st Century. Int J Mol Sci 3:401

  24. 24.

    Pereira JF, Gudina EJ, Doria ML, Domingues MR, Rodrigues LR, Teoxeira JA, Coutinho JA (2012) Characterization by electrospray ionization and tandem mass spectrometry of rhamnolipids produced by two Pseudomonas aeruginosa strains isolated from Brazilian crude oil. Eur J Mass Spectrom 18:399–406

  25. 25.

    Moya-Ramirez I, Tsaousi K, Rudden M, Marchant R, Jurado-Alameda E, Roman Garcia M, Banat IM (2015) Rhamnolipid and surfactin production from olive oil mill waste as sole carbon source. Bioresour Technol 198:231–236

  26. 26.

    Monteiro SA, Sassaki GL, De Souza LM, Meira JA, De Araujo JM, Mitchell DA, Ramos LP, Krieger N (2007) Molecular and structural characterization of the biosurfactant produced by Pseudomonas aeruginosa DAUPE 614. Chem Phys Lipids 147:1–13

  27. 27.

    Lovaglio RB, Dos Santos FJ, Junior MJ, Contiero J (2011) Rhamnolipid emulsifying activity and emulsion stability: pH rules. Colloids Surf B Biointerfaces 85:301–305

  28. 28.

    Zolfaghari R, Fakhru’l-Razi A, Abdullah LC, Elnashaie SS, Pendashteh A, (2016) Demulsification techniques of water-in-oil and oil-in-water emulsions in petroleum indusry. Sep Purif Technol 170:377–407

  29. 29.

    Zhao F, Shi R, Ma F, Han S, Zhang Y (2018) Oxygen effects on rhamnolipids production by Pseudomonas aeruginosa. Microb Cell Fact 17:39

  30. 30.

    Prabu R, Kuila A, Ravishankar R, Rao PV, Choudary NV, Velankar HR (2015) Microbial rhamnolipid production in wheat straw hydrolysate supplemented with basic salts. RSC Adv 5:51642–51649

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Acknowledgements

This work was supported by a grant provided by the Department of Science and Technology (DST), Science and Engineering Board (SERB), Govt. of India under a project file no. SB/YS/LS-83/2013. We also acknowledge with thanks the support extended by Dr. Prem. K. Gupta, Ionics clinical laboratory, Gurugram, Haryana for mass spectrometric analysis and Department of Biotechnology(DBT), Government of India for the High Field NMR spectrometer facility at the ICGEB, New Delhi. Authors wish to thank Dr Mathew Sawyer for his critical comments during manuscript preparation.

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Correspondence to Shashi Sharma.

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Joy, S., Rahman, P.K.S.M., Khare, S.K. et al. Production and characterization of glycolipid biosurfactant from Achromobacter sp. (PS1) isolate using one-factor-at-a-time (OFAT) approach with feasible utilization of ammonia-soaked lignocellulosic pretreated residues. Bioprocess Biosyst Eng 42, 1301–1315 (2019). https://doi.org/10.1007/s00449-019-02128-3

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Keywords

  • One-factor-at-a-time (OFAT)
  • Aqueous ammonia soaking (AAS)
  • Lignocellulosic residue
  • Rhamnolipid
  • Tandem-MS