Optimization of hyper-thermal acid hydrolysis and enzymatic saccharification of Ascophyllum nodosum for ethanol production with mannitol-adapted yeasts

  • InYung Sunwoo
  • Jeong Eun Kwon
  • Gwi-Tack Jeong
  • Sung-Koo KimEmail author
Research Paper


In this study, Ascophyllum nodosum was studied as a biomass for ethanol production. A. nodosum was degraded to monosaccharide by hyper-thermal (HT) acid hydrolysis and enzymatic saccharification and analyzed using response surface methodology (RSM) and the Michaelis–Menten equation. Maximum monosaccharide concentrations of 20.3 g/L glucose and 7.0 g/L mannitol were obtained from HT acid hydrolysis and enzymatic saccharification from 8%(w/v) of A. nodosum. Fermentation was conducted using Pichia stipitis and P. angophorae adapted to high mannitol concentrations. Neither non-adapted P. stipitis and P. angophorae nor adapted P. stipitis could ferment all mannitol in the A. nodosum hydrolysate. Adapted P. angophorae produced the highest ethanol concentration among various yeasts, with ethanol production reaching 13.6 g/L with an ethanol yield (YEtOH) of 0.50. Optimization of HT acid hydrolysis and enzymatic saccharification, in combination with the use of adapted yeast, could enhance overall A. nodosum ethanol fermentation yields.


Ascophyllum nodosum Hyper-thermal acid hydrolysis Enzymatic saccharification Adaptive evolution Ethanol production 



This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF), funded by the Ministry of Education (2016R1D1A1A09918683), Korea.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • InYung Sunwoo
    • 1
  • Jeong Eun Kwon
    • 1
  • Gwi-Tack Jeong
    • 1
  • Sung-Koo Kim
    • 1
    Email author
  1. 1.Department of BiotechnologyPukyong National UniversityBusanKorea

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