Production of cutinase by solid-state fermentation and its use as adjuvant in bioherbicide formulation
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In the present study, it was presented a strategy to maximize the cutinase production by solid-state fermentation from different microorganisms and substrates. The best results were observed using Fusarium verticillioides, rice bran being the main substrate. Maximum yield of cutinase obtained by the strain was 16.22 U/g. For concentration, ethanol precipitation was used, and the purification factor was 2.4. The optimum temperature and pH for enzyme activity were 35 °C and 6.5, respectively. The enzyme was stable at a wide range of temperature and at all pH values tested. The concentrated cutinase was used as an adjuvant in a formulation containing cutinase + bioherbicide. The use of enzyme increased the efficiency of bioherbicide, since cutinase was responsible to remove/degrade the cutin that recovery the weed leaves and difficult the bioherbicide absorption. Cutinase showed to be a promising product to be used in formulation of bioherbicides.
KeywordsAgroindustrial residues Ethanol precipitation Enzyme characterization Bioherbicide action
The authors thank CAPES for the scholarships as well as CNPq for the financial support of this work.
- 9.Cordeiro LG, El-Aouar ÂA, Gusmão RP (2012) Caracterização do bagaço de malte oriundo de cervejarias. Revista Verde de Agroecologia e Desenvolvimento Sustentável 7:20–22Google Scholar
- 12.De Almeida TC, Klaic R, Ariotti G, Sallet D, Spannemberg SS, Foletto EL, Kuhn RC, Hoffmann R, Mazutti MA (in press) Production and formulation of a bioherbicide from Phoma sp. as environment-friendly and safer alternative for weed control. Environ Qual ManagGoogle Scholar
- 14.Di Luccio M, Capra F, Ribeiro NP, Vargas GD, Freire DM, De Oliveira D (2003) Effect of temperature, moisture, and carbon supplementation on lipase production by solid-state fermentation of soy cake by Penicillium simplicissimum. In Proceedings of the Twenty-Fifth Symposium on Biotechnology for Fuels and Chemicals Held May 4–7, 2003, in Breckenridge, CO (pp. 173–180). Humana Press, Totowa, NJGoogle Scholar
- 24.Kolattukudy P, Poulose AJ (1996) Cutinase cleaning composition. U.S. Patent No. 5,512,203Google Scholar