Computer-aided design of microfluidic resistive network using circuit partition and CFD-based optimization and application in microalgae assessment for marine ecological toxicity
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We present an automatic design process for microfluidic dilution network towards marine ecological toxicity assessment on microalgae. Based on the hydraulic–electric circuit analogy, we defined an abstract specification using computer-aided designing system. Several approaches, especially circuit partition, were applied to minimize design effort. Computational fluid dynamics (CFD) simulation was exploited to convert the electrics specification to fabrication model. We automatically designed the combinational-mixing-serial dilution microfluidics to generate parallel stepwise gradients for mixing chemicals (binary/ternary/quaternary mixture) using the present algorithm. We critically discussed design rules and evaluated the microfluidic performance by colorimetric analysis. To examine whether these microfluidic chips can be used for toxicity test on microalgae, single and joint toxic effects of heavy metals (copper, mercury, zinc, and cadmium) were examined on line. In all cases, dose-related toxic responses were successfully detected. These results provided a solution for designing resistive network using circuit partition and CFD-based optimization and a route to develop a promising user-friendly alternative for microalgae bioassays as well as cell-based screening experiments in risk assessment.
KeywordsMicrofluidic network Computer-aided designing Circuit partition Microalgae bioassay
This work was supported by the National Natural Science Foundation of China (Nos. 41476085, 81471807), Scientific Research Project of Liaoning Education Department (LJQ2015005) and Dalian Innovation Project for Talents (2015R087).
Compliance with ethical standards
Conflict of interest
There is no conflict to declare.
- 15.Narayan KL, Rao KM, Sarcar MMM (2008) Computer aided design and manufacturing. Prentice-Hall, New DelhiGoogle Scholar
- 21.Klaine SJ, Lewis MA (1995) Algal and plant toxicity testing. Handbook of Ecotoxicology. Lewis Publishers, Boca RatonGoogle Scholar
- 27.Haskins CC (2008) Electricity made simple and treated non-technically. C Macdonald Press, LondonGoogle Scholar
- 28.Esposito A (1969) A simplified method for analyzing hydraulic circuits by analogy. Mach Des 41(24):173–177Google Scholar
- 29.Cimbala J, Cnegel Y (2006) Fluid mechanics: fundamentals and applications. McGraw-HillHigher Education, New YorkGoogle Scholar
- 32.Marking LL, Dawson VK (1975) Method of assessment of toxicity or efficacy of mixtures of chemicals. Center Integr Data Anal Wis Sci Center 67:1–8Google Scholar
- 35.Mohamed Gad-el-Hak (2002) The MEMS handbook. CRC Press, LondonGoogle Scholar
- 36.Berthier J, Silberzan P (2009) Microfluidics for biotechnology. Artech House, LondonGoogle Scholar
- 37.Associazione C, Delle I (2006) Microfluidics: history, theory and applications, vol 2. Springer, New York, pp 67–69Google Scholar
- 40.Dorf RC (1997) The electrical engineering handbook. CRC Press, New YorkGoogle Scholar
- 41.Gray PR (2001) Analysis and design of analog integrated circuits, vol 24(7). Wiley, New York, p 533Google Scholar
- 43.Tabeling P, Cheng S (2006) Introduction to microfluidics. Oxford University Press, OxfordGoogle Scholar
- 47.Duan J, Zhang Y, Zhao N, Yin G, Xiao X, Fang L, Liu W (2015) Joint toxicity of multi-heavy-metal to Chlorella pyrenoidosa based on 2 ~ 4 factorial design. J Atmos Environ Opt 10:376–385Google Scholar
- 48.Tian C (2010) Study on the discipline of algal fluorescence response to heavy metal toxicity. Dissertation for the master degree, Hebei University of Seience and TechnologyGoogle Scholar
- 49.Visviki I, Rachlin JW (1994) Acute and chronic exposure of Dunaliella salina and Chlamydomonas bullosa to copper and cadmium: effects on growth. Arch Environ Contam Toxicol 26:149–153Google Scholar
- 51.Maksimov VN, Gupta A (1992) The combined action of some heavy metals on marine phytoplankton. Vestn MGU (Biol) 2:51–57Google Scholar