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Bacterial indoleacetic acid-induced synthesis of colloidal Ag2O nanocrystals and their biological activities

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The biosynthesis and biological activity of colloidal Ag2O nanocrystals have not been well studied, although they have potential applications in many fields. For the first time, we developed a reducing agent free, cost-effective technique for Ag2O biosynthesis using Xanthomonas sp. P5. The optimal conditions for Ag2O synthesis were 50 °C, pH 8, and 2.5 mM AgNO3. Using these conditions the yield of Ag2O obtained at 10 h was about five times higher than that obtained at 12 h under unoptimized conditions. Ag2O was characterized by FESEM-EDS, TEM, dynamic light scattering, XRD, and UV–Visible spectroscopy. Indoleacetic acid produced by the strain P2 was involved in the synthesis of Ag2O. Ag2O exhibited a broad antimicrobial spectrum against several human pathogens. Furthermore, Ag2O exhibited 1,1-diphenyl-2-picrylhydrazyl (IC50 = 25.1 µg/ml) and 2,2′-azinobis-3-ethylbenzothiazoline-6-sulfonate (IC50 = 16.8 µg/ml) radical scavenging activities, and inhibited collagenase (IC50 = 27.9 mg/ml). Cytotoxicity of Ag2O was tested in fibroblast cells and found to be non-toxic, demonstrating biocompatibility.

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This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2015R1D1A1A01056919).

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Correspondence to Hong-Joo Son.

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Yoo, J., Jang, E., Jeong, S. et al. Bacterial indoleacetic acid-induced synthesis of colloidal Ag2O nanocrystals and their biological activities. Bioprocess Biosyst Eng 42, 401–414 (2019). https://doi.org/10.1007/s00449-018-2044-7

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  • Ag2O nanocolloids
  • Antimicrobial activity
  • Collagenase inhibition
  • Indoleacetic acid
  • Xanthomonas sp