Bioprocess and Biosystems Engineering

, Volume 42, Issue 2, pp 331–344 | Cite as

Outdoor microalgae cultivation in airlift photobioreactor at high irradiance and temperature conditions: effect of batch and fed-batch strategies, photoinhibition, and temperature stress

  • Suvidha Gupta
  • Sanjay B. PawarEmail author
  • R. A. Pandey
  • Gajanan S. Kanade
  • Satish K. Lokhande
Research Paper


The microalgae Scenedesmus abundans cultivated in five identical airlift photobioreactors (PBRs) in batch and fed-batch modes at the outdoor tropical condition. The microalgae strain S. abundans was found to tolerate high temperature (35–45 °C) and high light intensity (770–1690 µmol m− 2 s− 1). The highest biomass productivities were 152.5–162.5 mg L− 1 day− 1 for fed-batch strategy. The biomass productivity was drastically reduced due to photoinhibition effect at a culture temperature of > 45 °C. The lipid compositions showed fatty acids mainly in the form of saturated and monounsaturated fatty acids (> 80%) in all PBRs with Cetane number more than 51. The fed-batch strategies efficiently produced higher biomass and lipid productivities at harsh outdoor conditions. Furthermore, the microalgae also accumulated omega-3 fatty acid (C18:3) up to 14% (w/w) of total fatty acid at given outdoor condition.


Dark respiration Biodiesel Mixotrophic cultivation Airlift photobioreactor Fed-batch strategy 



The corresponding author is thankful to the Department of Science and Technology, New Delhi, India for their financial support for this research work under the scheme of DST INSPIRE Faculty Award (IFA13–ENG63). Ms. Suvidha Gupta is thankful to Council of Scientific and Industrial Research (CSIR), New Delhi, India for providing Senior Research Fellowship (SRF) to her for Ph.D. research work.

Supplementary material

449_2018_2037_MOESM1_ESM.doc (586 kb)
Supplementary material 1 (DOC 585 KB)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Suvidha Gupta
    • 1
    • 2
  • Sanjay B. Pawar
    • 2
    Email author
  • R. A. Pandey
    • 3
  • Gajanan S. Kanade
    • 4
  • Satish K. Lokhande
    • 4
  1. 1.Academy of Scientific and Innovative Research (AcSIR)GhaziabadIndia
  2. 2.Environmental Biotechnology and Genomics DivisionCSIR-National Environmental Engineering Research Institute (NEERI)NagpurIndia
  3. 3.CSIR-National Environmental Engineering Research Institute (NEERI)NagpurIndia
  4. 4.Analytical Instruments DivisionCSIR-National Environmental Engineering Research Institute (NEERI)NagpurIndia

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