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Insight into Pseudomonas aeruginosa pyocyanin production under low-shear modeled microgravity

  • Sunirmal Sheet
  • Yesupatham Sathishkumar
  • Mi-Sook Choi
  • Yang Soo Lee
Research Paper

Abstract

Long-term space flight impairs the immune system of astronauts, rendering them vulnerable to opportunistic infections. Pseudomonas aeruginosa causes opportunistic infections, particularly in individuals with a compromised immune system; it can be a major health hazard for astronauts during space flight missions. Hence, the production of the most abundant redox active virulence factor, pyocyanin by P. aeruginosa, was assessed under low-shear modeled microgravity (LSMMG) conditions, simulated using a high aspect ratio vessel. Moreover, we evaluated changes in the expression of genes involved in pyocyanin biosynthesis and genes involved in the MexGHI-OpmD operon quorum sensing. Extracellular DNA and H2O2 production were measured, and their correlation with pyocyanin production was examined. Interestingly, the pyocyanin quantity was 2.58-fold lower in the LSMMG conditions compared to the normal gravity. LSMMG caused downregulation of the genes associated with pyocyanin biosynthesis. Interestingly, extracellular DNA and H2O2 release were significantly high in the normal gravity environment. Scanning electron microscopy revealed aggregation and elongated cells under LSMMG. Taken together, these findings suggest that LSMMG did not induce pyocyanin secretion in P. aeruginosa.

Keywords

Low-shear modeled microgravity Pyocyanin Pseudomonas aeruginosa 

Notes

Acknowledgements

The SEM samples were analyzed using the JEM-2010 (JEOL) installed at the Center for University-Wide Research Facilities (CURF) at Chonbuk National University. We thank Mrs. Eun-Jin Choi at the CURF at Chonbuk National University for SEM analysis.

Funding

This research was supported by Buan RIS Resource Project (Grand no. R0001102) and funds of Chonbuk National University.

Compliance with ethical standards

Conflict of interest

Authors do not have any conflicts of interest.

Supplementary material

449_2018_2031_MOESM1_ESM.doc (31 kb)
Supplementary material 1 (DOC 31 KB)
449_2018_2031_MOESM2_ESM.tif (117 kb)
Supplementary material 2 (TIF 116 KB)
449_2018_2031_MOESM3_ESM.tif (423 kb)
Supplementary material 3 (TIF 422 KB)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Sunirmal Sheet
    • 1
  • Yesupatham Sathishkumar
    • 1
    • 2
  • Mi-Sook Choi
    • 1
  • Yang Soo Lee
    • 1
  1. 1.Department of Forest Science and Technology, College of Agriculture and Life SciencesChonbuk National UniversityJeonju-siRepublic of Korea
  2. 2.Department of Biological SciencesKorea Advanced Institute of Science and TechnologyDaejeonRepublic of Korea

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