Bioprocess and Biosystems Engineering

, Volume 42, Issue 1, pp 131–141 | Cite as

Alginate immobilization of Morus alba L. cell suspension cultures improved the accumulation and secretion of stilbenoids

  • Chadathorn Inyai
  • Panitch Boonsnongcheep
  • Jukrapun Komaikul
  • Boonchoo Sritularak
  • Hiroyuki Tanaka
  • Waraporn PutalunEmail author
Research Paper


Morus alba L. (Moraceae) has been used in traditional medicine for the treatment of several illnesses. Recent research also revealed several pharmacological activities from many groups of secondary metabolites, including the stilbenoids mulberroside A, oxyresveratrol, and resveratrol, which are promising compounds for cosmetic and herbal supplement products. In our previous study, cell cultures of M. alba showed high productivity of these compounds. In this study, we attempted to develop immobilized cell cultures of M. alba and to test the effect of elicitors and precursors on the production of stilbenoids. The immobilization of the M. alba cells significantly promoted the secretion of mulberroside A into the extracellular matrix and culture media to 60%, while enhancing the level of oxyresveratrol and resveratrol by 12- and 27-fold, respectively. The elicitation of immobilized cells with a combination of 50 µM methyl jasmonate and 0.5 mg/mL yeast extract for 24 h promoted a twofold increase in the production of all three stilbenoids. Furthermore, the addition of 0.05 mM l-phenylalanine, 0.03 mM l-tyrosine, or a combination resulted in the enhancement of mulberroside A production for up to twofold. The addition of l-tyrosine significantly enhanced the production of oxyresveratrol and resveratrol. This is the first report of stilbenoid production using immobilized cell cultures of M. alba. The cultures have benefits over normal cell suspension cultures by promoting the secretion of mulberroside A and enhancing the levels of oxyresveratrol and resveratrol. Thus, it could be a candidate method for the production of these stilbenoids.


Plant tissue culture Immobilization Stilbenoids Elicitation Precursor feeding 



The authors wish to thank the National Research Council of Thailand and the Japan Society for the Promotion of Sciences (NRCT-JSPS) joint research program; the Graduate School, Khon Kaen University; the Faculty of Pharmaceutical Sciences, Khon Kaen University; and a scholarship under the Postdoctoral Training Program from the Research Affairs and Graduate School, Khon Kaen University, Thailand (Grant no. 58439-2).


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Chadathorn Inyai
    • 1
    • 2
  • Panitch Boonsnongcheep
    • 1
    • 2
  • Jukrapun Komaikul
    • 1
    • 2
  • Boonchoo Sritularak
    • 3
  • Hiroyuki Tanaka
    • 4
  • Waraporn Putalun
    • 1
    • 2
    Email author
  1. 1.Faculty of Pharmaceutical SciencesKhon Kaen UniversityKhon KaenThailand
  2. 2.Research Group for Pharmaceutical Activities of Natural Products using Pharmaceutical Biotechnology (PANPB), National Research UniversityKhon Kaen UniversityKhon KaenThailand
  3. 3.Faculty of Pharmaceutical SciencesChulalongkorn UniversityBangkokThailand
  4. 4.Graduate School of Pharmaceutical SciencesKyushu UniversityFukuokaJapan

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